Addgene

We narrowed to 293 results for: Ecd

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p031 pCS2 End-1 ORF

Plasmid

#11030

Depositor

Ramesh Shivdasani

Article

Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

Insert

End-1 (end-1 Nematode)

Use

Xenopus, mammalian, avian, and zebrafish

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pTJV1Sc-rpoB

Plasmid

#166978

Purpose

Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance

Depositor

Christopher Reisch

Article

Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

Inserts

Ec86 Retron (ECD_00831 )
lambda bet

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23101 and VanCC

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pDBEcR/Bla

Plasmid

#201556

Purpose

Plasmid with SB ITRs for expressing DBEcR (3xmyc-tagged) under EF1α promoter; blasticidin resistance.

Depositor

Randy Poon

Article

Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

Insert

DBEcR

Use

Unspecified; Sleeping beauty transposon

Tags

Myc

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 13, 2023

Availability

Academic Institutions and Nonprofits only

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p026 pCS2-EnR

Plasmid

#11028

Depositor

Ramesh Shivdasani

Article

Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

Insert

engrailed (en Fly)

Use

Xenopus, mammalian, avian, and zebrafish

Tags

Expression

Mutation

Engrailed repressor domain (bases encoding amino …

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pCI-neo-RL-ZEB1

Plasmid

#35535

Depositor

Greg Goodall

Article

Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

Insert

3'UTR of ZEB1 (ZEB1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2012

Availability

Academic Institutions and Nonprofits only

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pCI-neo-RL-ZEB1 200bmutx5

Plasmid

#35537

Depositor

Greg Goodall

Article

Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. E

Insert

3'UTR of ZEB1 (ZEB1 Budding Yeast, Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

5 mutations in miR200b seed regions of 3'UTR…

Promoter

Available since

May 10, 2012

Availability

Academic Institutions and Nonprofits only

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KHBD00332

Plasmid

#34455

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG1765 (EcR Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
p029 End-1::EnR

Plasmid

#11029

Depositor

Ramesh Shivdasani

Article

Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

Insert

End1::EnR (end-1, en END-1 is from C. Elegans, EnR is from Drophila)

Use

Xenopus, mammalian, avian, and zebrafish

Tags

Expression

Mutation

Dominant inhibitory GATA-binding protein End1::En…

Promoter

Available since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only

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BPV00914 pET21GG2 H6-TF-BECN (1-450) [VA-MA-LA]

Plasmid

#136688

Purpose

Expresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor). Three mutations are added to the CC and ECD/BARA interface.

Depositor

Colin Garvie

Article

Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

Insert

Beclin-1 (BECN1 Human)

Use

Tags

Trigger Factor

Expression

Bacterial

Mutation

DNA optimized for E. coli expression w/ three mut…

Promoter

T7

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pIND-GFP

Plasmid

#11612

Depositor

Steven Johnson

Insert

Green Fluorescent Protein

Use

Ecdysone inducible

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2007

Availability

Academic Institutions and Nonprofits only