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Plasmid#185686PurposeLow-level transient expression of a HaloTag protein targeted to the ER membrane, fused with a GST for constitutive dimerization.DepositorInsertERmembrane-GST-HaloTag-KKMP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-EMB-COMP5AP-AviTag-9xHis
Plasmid#157244PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertEMB (EMB Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-EMB-Fc(DAPA)-AviTag-6xHis
Plasmid#156680PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertEMB (EMB Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 Assembly Register 3/3 Part - 5'UTR:Luc:Tnos (GB1500)
Plasmid#160581PurposePart 3/3 for assembly of site-specific recombination based Registers. Composed of the 5'UTR sequence of 35S promoter, coding sequence of Luc and NOS terminatorDepositorInsertLuc
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK5 FLAG membrane-bound lipin-1 (delta200)
Plasmid#120278PurposeExpression of ER/Golgi-tethered lipin-1 in mammalian cellsDepositorInsertLipin-1 (Lpin1 Mouse)
UseTagsFLAG and in-frame fusion with the delta200 Golgi-…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMOS009: QuasAr2-mOrange2 voltage sensor (plasma membrane)
Plasmid#163043PurposeQuasAr2-mOrange2 voltage sensor (plasma membrane)DepositorInsertQuasAr2
UseLentiviralTagsmOrange2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOS010E: Entry vector for: CheRiff-eGFP channelrhodopsin (plasma membrane)
Plasmid#163063PurposeEntry vector for: CheRiff-eGFP channelrhodopsin (plasma membrane)DepositorInsertCheRiff
UseGateway entry vectorTagseGFPExpressionMutationPromoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
p416-TEF-membrane localization signal-tagBFP-wrmScarlet11-TEF terminator
Plasmid#158585PurposeExpresses membrane localized tagBFP with wrmScarlet11 C-terminal fusion in S. cerevisiaeDepositorInsertmembrane localization signal-tagBFP-wrmScarlet11
UseTagstagBFPExpressionYeastMutationGGGS linkerPromoterTEFAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMOS009E: Entry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)
Plasmid#163062PurposeEntry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)DepositorInsertQuasAr2
UseGateway entry vectorTagsmOrange2ExpressionMutationPromoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
PUF Assembly Kit
Plasmid Kit#1000000051PurposeUsed to assemble custom-made RNA-binding proteins for recognition of 8-base long targets within RNA. The kit is based on the Pumilio and FBF (PUF) homology domain of the full-length human Pumilio 1.DepositorAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only