1. p426 46Q GPD

   Plasmid

   #1182

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 46Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

These results may also be relevant.

1. BS.YxiN.Twin1.a1-368.wt

   Plasmid

   #13528

   Depositor

   David McKay

   Article

   Karginov et al J Biol Chem. 2005 Oct 21. 280(42):35499-

   Insert

   B. subtilis DEAD-box helicase YxiN first two domains
   

   Use

   Tags

   intein-chitin binding domain

   Expression

   Bacterial

   Mutation

   deleted amino acid residues 369-479

   Promoter

   Available since

   Feb. 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. BS.YxiN.Twin1.a404-479.wt

   Plasmid

   #13529

   Depositor

   David McKay

   Article

   Karginov et al J Biol Chem. 2005 Oct 21. 280(42):35499-

   Insert

   B. subtilis DEAD-box helicase YxiN specific RNA binding domain
   

   Use

   Tags

   intein-chitin binding domain

   Expression

   Bacterial

   Mutation

   delete amino acid residues 1-403

   Promoter

   Available since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Traptavidin-E6

   Plasmid

   #59549

   Purpose

   pET21-Tre for expression of traptavidin (high stability mutant of streptavidin) bearing a glutamic acid tag for ion-exchange chromatography

   Depositor

   Mark Howarth

   Article

   Fairhead et al J Am Chem Soc. 2014 Sep 3;136(35):12355-

   Insert

   Core Traptavidin-E6
   

   Use

   Tags

   E6 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pc DNA FRT TO- APP695

   Plasmid

   #114193

   Purpose

   Creation of a stable cell line expressing APP mutation (AD in vitro study)

   Depositor

   Aleksandra Radenovic

   Insert

   Amyloid-Precursor-Protein (APP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   wild type splice variant 695: 695AA (2085bp, lack…

   Promoter

   CMV

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTM1_VP35_ZEBOV

   Plasmid

   #68121

   Purpose

   Encodes for Ebola Virus Zaire VP35 under control of a T7 RNA polymerase promoter.

   Depositor

   Elke Mühlberger

   Article

   Trunschke et al Virology. 2013 Jul 5;441(2):135-45. doi:

   Insert

   VP35 ZEBOV (VP35 )
   

   Use

   T7

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pWZL-Blast-Flag-HA-hTERT

   Plasmid

   #22396

   Depositor

   William Hahn

   Article

   Maida et al Nature. 2009 Sep 10. 461(7261):230-5.

   Insert

   TERT (TERT Human)
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTM1_VP30_ZEBOV

   Plasmid

   #69119

   Purpose

   Encodes for Ebola Virus Zaire VP30 under control of a T7 RNA polymerase promoter

   Depositor

   Elke Mühlberger

   Article

   Trunschke et al Virology. 2013 Jul 5;441(2):135-45. doi:

   Insert

   VP30 ZEBOV (VP30 )
   

   Use

   T7

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAcGFP1-N1-MAHS

   Plasmid

   #90034

   Purpose

   To express GFP-tagged MAHS in mammalian cells transiently

   Depositor

   Takekazu Kunieda

   Article

   Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

   Insert

   MAHS
   

   Use

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEF-TAZ-N-Flag

   Plasmid

   #19025

   Depositor

   Michael Yaffe

   Article

   Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

   Insert

   TAZ (Wwtr1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Vector has no SV40 origin

   Promoter

   Available since

   Aug. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pAcGFP1-N1-RvLEAM

    Plasmid

    #90035

    Purpose

    To express GFP-tagged RvLEAM in mammalian cells transiently

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

    Insert

    RvLEAM
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAG426-GAL-APP-CTF

    Plasmid

    #181732

    Purpose

    Galactose inducible expression of APP-CTF

    Depositor

    Alejandro Chavez

    Insert

    APP-CTF (APP Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    C-terminal fragment (aa 671-770)

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p667-UBC-STKLD1-V5-miniTurbo_IDG-K

    Plasmid

    #189916

    Purpose

    Gateway destination clone of STKLD1 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    STKLD1 (C9orf96 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPRIChp-hOtub1

    Plasmid

    #174743

    Purpose

    Gene overexpression

    Depositor

    Shao-Cong Sun

    Article

    Zhou et al Nat Immunol. 2019 Jul;20(7):879-889. doi

    Insert

    OTUB1 (OTUB1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDONR221_HUWE1_isoform1

    Plasmid

    #190464

    Purpose

    Entry clone

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Use

    Entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAGGS_L_EBOV

    Plasmid

    #103052

    Purpose

    Encodes Ebola virus L gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

    Depositor

    Elke Mühlberger

    Article

    Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

    Insert

    L gene of Ebola virus (L Zaire ebolavirus, Mayinga)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAGGS_VP35_EBOV

    Plasmid

    #103050

    Purpose

    Encodes Ebola virus VP35 gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

    Depositor

    Elke Mühlberger

    Article

    Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

    Insert

    VP35 gene of Ebola virus (VP35 (Zaire ebolavirus, Mayinga))
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAGGS_VP30_EBOV

    Plasmid

    #103051

    Purpose

    Encodes Ebola virus VP30 gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

    Depositor

    Elke Mühlberger

    Article

    Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

    Insert

    VP30 gene of Ebola virus (VP30 Zaire ebolavirus, Mayinga)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEThT-RvLEAM

    Plasmid

    #90033

    Purpose

    To express His-tagged RvLEAM in bacteria

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

    Insert

    RvLEAM
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p3xFLAG-ATF6

    Plasmid

    #11975

    Purpose

    For mammalian expression of human ATF6 with a 3x FLAG tag

    Depositor

    Ron Prywes

    Article

    Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-

    Insert

    ATF6 (ATF6 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP-ATF6

    Plasmid

    #32955

    Purpose

    For visualization of GFP-ATF6 in mammalian cells.

    Depositor

    Ron Prywes

    Article

    Chen et al J Biol Chem. 2002 Apr 12. 277(15):13045-

    Insert

    ATF6 (ATF6 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    M67L and M313I

    Promoter

    CMV

    Available since

    Nov. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge