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Plasmid#197728PurposePhytobrick (MoClo) Level 0 PartDepositorInsertIntegrase13 (tomato optimized)
UseTagsExpressionPlantMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRD411
Plasmid#178196PurposesRok overexpressionDepositorInsertsrok
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRD231
Plasmid#178195PurposeRok overexpressionDepositorInsertrok
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDR111_mannose_Bgal NLS
Plasmid#188398PurposeExpresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.DepositorInsertlacZ
UseTagsPhoD secretion peptide and SV40 NLSExpressionBacterialMutationPromoterD-mannoseAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-bhaA
Plasmid#167810PurposeExpresses His6-tagged BhaA in E. coliDepositorInsertBhaA
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-bhaB1
Plasmid#167811PurposeExpresses His6-tagged BhaB1 in E. coliDepositorInsertBhaB1
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-bhaB7
Plasmid#167813PurposeExpresses His6-tagged BhaB7 in E. coliDepositorInsertBhaB7
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15b-bhaC1
Plasmid#167814PurposeExpresses BhaC1 in E. coliDepositorInsertBhaC1
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJT121_GalL_nCDA1-VQRBE3
Plasmid#145086PurposeExpressing base editor nCDA1-VQRBE3 in yeast cellsDepositorInsertnCDA1-VQRBE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A, D1135V, R1335Q, T1337R)PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFE-baDAB
Plasmid#133003PurposepFE carrying the badabA and badabB genesDepositorInsertsbadabB
vcdabA
UseTagsExpressionBacterialMutationPromotertetRAvailable sinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
BS.YxiN.Twin1.a1-368.wt
Plasmid#13528DepositorInsertB. subtilis DEAD-box helicase YxiN first two domains
UseTagsintein-chitin binding domainExpressionBacterialMutationdeleted amino acid residues 369-479PromoterAvailable sinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
BS.YxiN.Twin1.a404-479.wt
Plasmid#13529DepositorInsertB. subtilis DEAD-box helicase YxiN specific RNA binding domain
UseTagsintein-chitin binding domainExpressionBacterialMutationdelete amino acid residues 1-403PromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI Probe
Plasmid#211507PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol (PI) from bacteriaDepositorInsertBcPI-PLC-ANH
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJC175e
Plasmid#79219PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPPSP gIII, luxAB
UseTagsExpressionBacterialMutationPromoterPPSPAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJT130_GalL_nCDA1-NGBE3
Plasmid#145094PurposeExpressing base editor nCDA1-NGBE3 in yeast cellsDepositorInsertnCDA1-NGBE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcpBpaRS_v2
Plasmid#196485PurposeTo express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.DepositorInsertbenzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
UseTagsExpressionMammalianMutationPromoterSV40(synthetase), Pol III tRNA promoter (tRNA), C…Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAB061i
Plasmid#79226PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPpro1 rpoZ-HA4; PlacIq lacI
UseTagsExpressionBacterialMutationPromoterPpro1; PlacIqAvailable sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAB061i3
Plasmid#79230PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPpro1 rpoZ; PlacIq lacI
UseTagsExpressionBacterialMutationPromoterPpro1; PlacIqAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcpAzpaRS_v2
Plasmid#196483PurposeTo express azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into azidophenylalanine.DepositorInsertazidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS), amber suppressor tRNA
UseTagsExpressionMammalianMutationPromoterSV40(synthetase), Pol III tRNA promoter (tRNA), C…Available sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAB078d3
Plasmid#79203PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) luxAB; Ppro1 434cI-SH2ABL1
UseTagsExpressionBacterialMutationPromoterPlacZ-opt (OR1); Ppro1Available sinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only