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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 698 results for: Bacillus

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Showing: 241 - 260 of 698 results

  2. pcpBpaRS

    Plasmid
    #196484
    Purpose
    To express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.
    Depositor
    Kensaku Sakamoto
    Article
    Hino et al Methods Mol Biol. 2012;794:215-28. doi:
    Insert
    benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
    Expression
    Mammalian
    Promoter
    CMV (synthetase), Pol III tRNA promoter (tRNA)
    Available Since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    196484-plasmid-map-sequence-id-388864

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  4. pDR111_Bgal NLS

    Plasmid
    #188396
    Purpose
    Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.
    Depositor
    Christopher Contag
    Article
    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:
    Insert
    lacZ
    Tags
    PhoD secretion peptide and SV40 NLS
    Expression
    Bacterial
    Promoter
    Phyper spank
    Available Since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    188396-plasmid-map-sequence-id-373478

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  10. pET11a-DAAT-V33G/T242G

    Plasmid
    #159278
    Purpose
    Aminotransferase variant with near-native catalytic efficiency toward D-tryptophan
    Depositor
    Roberto Chica
    Article
    Parmeggiani et al ACS Catalysis 9, 3482–3486.
    Insert
    Engineered d-alanine aminotransferase
    Tags
    6x His tag
    Expression
    Bacterial
    Mutation
    Valine 33 and threonine 242 have been mutated to …
    Promoter
    T7
    Available Since
    Sept. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    159278-plasmid-map-sequence-id-311390

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Showing: 241 - 260 of 698 results