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Showing: 241 - 260 of 297 results

  3. pNSSCPG

    Plasmid
    #183187
    Purpose
    PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; sfGFP-LAA expressed under T7 p
    Depositor
    Hsiao-Chun Huang
    Article
    Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1
    Inserts
    superfolder GFP
    SpmX450 and T7 181+ fusion protein
    T7 RNAP 1-179 variant and SpmX450 fusion protein
    mRFP1 and popZ fusion protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    183187-plasmid-map-sequence-id-366657

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  5. pLenti NLuc398-G8NCRD IRES G8NCRD-CLuc394

    Plasmid
    #128386
    Purpose
    Expresses a split firefly luciferase reporter based on the N terminal carbohydrate recognition domain of human galectin 8, which concentrates inside endosomes following endosomal disruption
    Depositor
    Craig Duvall
    Article
    Kilchrist et al ACS Sens. 2020 Jun 23. doi: 10.1021/acss
    Inserts
    NLuc-3xGGGGS-Gal8-NCRD (LGALS8 Synthetic, Human)
    Gal8-NCRD-3xGGGGS-CLuc398 (LGALS8 Human)
    Use
    Lentiviral, Luciferase, and Synthetic Biology
    Tags
    C-terminal luc2 fragment and N-terminal luc2 frag…
    Expression
    Mammalian
    Mutation
    N terminal carbohydrate recognition domain fused …
    Promoter
    EF1A and IRES
    Available since
    Jan. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    128386-plasmid-map-sequence-id-263327

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  7. pCZGY3196

    Plasmid
    #172696
    Purpose
    Prgef-1-gfp1-10 Expresses split GFP parts 1-10 in all C. elegans neurons (only shows active fluorescence when binds to GFP11)
    Depositor
    Yishi Jin
    Article
    Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1
    Insert
    Prgef-1
    Use
    Tags
    GFP 1-10
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    172696-plasmid-map-sequence-id-346674

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  9. pCZGY3195

    Plasmid
    #172695
    Purpose
    Punc-25-gfp1-10 Expresses split GFP parts 1-10 specifically in C. elegans GABAergic motor neurons (only shows active fluorescence when binds to GFP11)
    Depositor
    Yishi Jin
    Article
    Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1
    Insert
    Punc-25
    Use
    Tags
    GFP 1-10
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Nov. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    172695-plasmid-map-sequence-id-346669

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  14. pNSSCP

    Plasmid
    #183188
    Purpose
    PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; DivIVA-sfGFP expressed under T
    Depositor
    Hsiao-Chun Huang
    Article
    Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1
    Inserts
    DivIVA and superfolder GFP fusion protein
    SpmX450 and T7 181+ fusion protein
    T7 RNAP 1-179 variant and SpmX450 fusion protein
    mRFP1 and popZ fusion protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    183188-plasmid-map-sequence-id-366663

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Showing: 241 - 260 of 297 results