We narrowed to 59,421 results for: cloning

1. pME-memGFP-P2A

   Plasmid

   #80809

   Purpose

   Fyn myristoylation domain fused to N-terminus of GFP (memGFP) without stop codon, followed by P2A

   Depositor

   Philip Washbourne

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   pME-memGFP-P2A
   

   Expression

   Mammalian

   Available Since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. Hs.KRAS4b G12R

   Plasmid

   #83143

   Purpose

   Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12R mutation

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Mutation

   G12R

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIIS18-BsaI

   Plasmid

   #128649

   Purpose

   Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

   Depositor

   Tohru Suzuki

   Article

   Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   Oct. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMB4

   Plasmid

   #122246

   Purpose

   Lentivirus delivery for stable expression of As crRNA, has AsDR; Cloning vector for expression of AsCpf1 crRNA. It contains BsmBI site for cloning.

   Depositor

   Darjus Tschaharganeh

   Article

   Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Hs.KRAS4b S17N

   Plasmid

   #83156

   Purpose

   Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), S17N mutation

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Mutation

   S17N

   Available Since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pME-nlsGFP-P2A

   Plasmid

   #80808

   Purpose

   nuclear localization signal fused to N-terminus of GFP (nlsGFP) without stop codon, followed by P2A

   Depositor

   Philip Washbourne

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   minimal cytomegalovirus immediate early enhancer/promoter
   

   Available Since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCoofy48

   Plasmid

   #55186

   Purpose

   Bacterial expression vector for parallel SLIC cloning containing N-terminal 6x His and GST tags. C-terminal tags can be added during SLIC.

   Depositor

   Sabine Suppmann

   Article

   Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

   Type

   Empty backbone

   Tags

   6x His, GST, His10-Stop-OneStrep-Stop-S-tag-Stop-…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. Hs.KRAS4b Q61L

   Plasmid

   #83134

   Purpose

   Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61L mutation

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Mutation

   Q61L

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR221-CAG.Pro

   Plasmid

   #79490

   Purpose

   MultiSite Gateway entry clones, first fragment, (attL1, attR5) CAG promoter

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   CAG Promoter
   

   Use

   Multisite gateway entry clone

   Promoter

   CAG Promoter

   Available Since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGolden-Hyg

    Plasmid

    #51423

    Purpose

    Contain one loxP-PGK-Hyg-pA-loxP cassette, used for golden-gate reaction

    Depositor

    Yonglun Luo

    Article

    Luo et al Biotechniques. 2014 May 1;56(5):263-8. d

    Insert

    PGK-Hyg-pA (PGK1 Human)
    

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. BSP188

    Plasmid

    #110917

    Purpose

    For cloning by HR in yeast, Universal MosSCI compatible, has unc54 terminator cloned

    Depositor

    Alexander Mendenhall

    Article

    Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

    Insert

    unc54 Terminator
    

    Use

    Synthetic Biology; Yeast homologous recombination…

    Expression

    Worm

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Hs.KRAS4b G13C

    Plasmid

    #83145

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G13C mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    G13C

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. Hs.KRAS4b Q61R

    Plasmid

    #83135

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61R mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    Q61R

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. Hs.KRAS4a G12V

    Plasmid

    #83169

    Purpose

    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), G12V mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    G12V

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. [GG][Pin]

    Plasmid

    #97191

    Purpose

    Pin nuclease domain with Pum cloning site

    Depositor

    Edward Boyden

    Article

    Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Insert

    Pin domain
    

    Use

    Synthetic Biology

    Tags

    Pum cloning site

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCoofy50

    Plasmid

    #55189

    Purpose

    Mammalian expression vector for parallel SLIC cloning containing N-terminal HA, 8x His and FLAG tags. C-terminal tags can be added during SLIC.

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Tags

    8x His, FLAG, HA, PreScission site (3C protease s…

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Aug. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. p3E-mVenus

    Plasmid

    #67719

    Purpose

    Multisite gateway entry clone for adding C-terminal fusions of mVenus (no ATG)

    Depositor

    Rob Parton

    Article

    Morsch et al Front Cell Neurosci. 2015 Aug 31;9:321.

    Insert

    mVenus
    

    Use

    Gateway multisite 3' entry clone

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. Hs.KRAS4a G12C

    Plasmid

    #83167

    Purpose

    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), G12C mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    G12C

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR-D-TOPO-PAmCherry1_160-236-MCS

    Plasmid

    #60546

    Purpose

    Cloning PAmCherry1 C-terminal fragment (RC) for BiFC-PALM

    Depositor

    Xiaolin Nan

    Article

    Nickerson et al PLoS One. 2014 Jun 25;9(6):e100589. doi:

    Insert

    PAmCherry1 C-terminal fragment
    

    Use

    Cloning

    Mutation

    amino acids 160-236

    Available Since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR-D-TOPO-PAmCherry1_1-159-MCS

    Plasmid

    #60545

    Purpose

    Cloning PAmCherry1 N-terminal fragment (RN) for BiFC-PALM

    Depositor

    Xiaolin Nan

    Article

    Nickerson et al PLoS One. 2014 Jun 25;9(6):e100589. doi:

    Insert

    PAmCherry1 N-terminal fragment
    

    Use

    Cloning

    Mutation

    amino acids 1-159

    Available Since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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