We narrowed to 10,152 results for: yeast

1. Ypq2-2GFP-URA

   Plasmid

   #207010

   Purpose

   Integration of 2xGFP tag at YPQ2 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   YPQ2
   

   Tags

   2xGFP

   Expression

   Yeast

   Available Since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Vph1-2GFP-URA

   Plasmid

   #207009

   Purpose

   Integration of 2xGFP tag at VPH1 C terminus, use auxotrophic marker URA3(Kluyveromyces lactis) for selection. Marker for yeast vacuolar membrane.

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   VPH1
   

   Tags

   2xGFP

   Expression

   Yeast

   Available Since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. p426-Cup1p-FusionRed

   Plasmid

   #188392

   Purpose

   Cu2+-dependent expression of red fluorescent protein in yeast cells

   Depositor

   Michihiko Kataoka

   Article

   Utsumi et al PLoS One. 2023 Apr 13;18(4):e0283002. do

   Insert

   FusionRed
   

   Expression

   Yeast

   Promoter

   CUP1 promoter

   Available Since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. p21StBD

   Plasmid

   #190269

   Purpose

   Constitutively expresses a protein of interest fused to a GAL4 DNA-binding domain. Targeted integration to genomic locus YPRC-tau 3, Chromosome XVI.

   Depositor

   Assaf Mosquna

   Article

   Zimran et al ACS Synth Biol. 2022 Aug 19;11(8):2880-2

   Type

   Empty backbone

   Use

   Synthetic Biology; Yeast genome-integrating vector

   Tags

   GAL4-BD

   Promoter

   TDH3

   Available Since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. deltaVP1 + VP2C-MIOX

   Plasmid

   #166680

   Purpose

   Yeast integrative plasmid for expressing deltaVP1 (GAL1 promoter) and mouse myo-inositol oxygenase tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

   Inserts

   VP2C-MIOX
   Murine polyomavirus deltaVP1 (NLS deletion mutant)
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   GAL1 and GAL10

   Available Since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCu-His6-Atg13[571–700] F641E, I645E-Ss(424)

   Plasmid

   #166854

   Purpose

   Expresses 6xHis tagged ATG13 [571–700] fragment mutated in F641E, I645E with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

   Insert

   Atg13[571–700] (ATG13 Budding Yeast)
   

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   F641E, I645E

   Promoter

   pCu

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCu-His6-Atg13[571–700] F641G, I645G-Ss(424)

   Plasmid

   #166853

   Purpose

   Expresses 6xHis tagged ATG13 [571–700] fragment mutated in F641G, I645G with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

   Insert

   Atg13[571–700] (ATG13 Budding Yeast)
   

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   F641G, I645G

   Promoter

   pCu

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pK_lacO-cP-AOX1-AtX+NpgA

   Plasmid

   #126727

   Purpose

   yeast plasmid overexpressing AtX and NpgA

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   atX,npgA
   

   Tags

   6xHis

   Expression

   Yeast

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKdH_P-GAP-ScADH2

   Plasmid

   #126734

   Purpose

   yeast plasmid overexpressing ADH2 from Saccharomyces cerevisiae

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   adh2
   

   Tags

   6xHis

   Expression

   Yeast

   Promoter

   GAP

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pK_-P-GAP-NpgA+AtX

    Plasmid

    #126725

    Purpose

    yeast plasmid overexpressing AtX and NpgA

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    atX,npgA
    

    Tags

    6xHis

    Expression

    Yeast

    Promoter

    GAP

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZ_lacO-cP-AOX1-LovC

    Plasmid

    #126729

    Purpose

    yeast plasmid overexpressing LovC

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    lovC
    

    Tags

    6xHis

    Expression

    Yeast

    Available Since

    June 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZ_lacO-cP-AOX1-LovB

    Plasmid

    #126728

    Purpose

    yeast plasmid overexpressing LovB

    Depositor

    Menghao Cai

    Article

    Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

    Insert

    lovB
    

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    T7227A,T8994A

    Available Since

    June 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKP134 [pRS406-YBR139Wp-YBR139W(S219,D415,H474A)-GFP-ADH1t]

    Plasmid

    #106469

    Purpose

    Expresses Atg42/Ybr139w(S219,D415,H474A) with a C-terminal GFP tag in yeast cells

    Depositor

    Daniel Klionsky

    Article

    Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

    Insert

    YBR139W(S219,D415,H474A)
    

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    Changed Serine 219, Aspartate 415 and Histidine 4…

    Promoter

    YBR139W

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRS424-TUB2 - human TUBB3 Cterminal tail delta K

    Plasmid

    #60404

    Purpose

    Expression of chimeric yeast beta tubulin (TUB2) - human beta3 tubulin Cterminal tail (TUBB3) without last 4 amino acids--QGPK

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB2
    

    Expression

    Yeast

    Mutation

    TUB2 amino acids 429 - end, replaced with human T…

    Promoter

    GAL

    Available Since

    May 6, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pAV10.K3.loxP

    Plasmid

    #63202

    Purpose

    pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAV10.K5.loxP

    Plasmid

    #63204

    Purpose

    pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into YKL162C-A (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAV10.HO3.loxP

    Plasmid

    #63205

    Purpose

    pCACS backbone (Amp), SpHIS5, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. LoxP flanked TU. Integration into HO locus (NotI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAV10.HO6

    Plasmid

    #63211

    Purpose

    pCACS backbone (Amp), KlURA3, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into HO locus (NotI or BciVI).

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIIIA/MS2-1

    Plasmid

    #220631

    Purpose

    For expressing an RNA containing your sequence of interest adjacent to two MS2 sites from an RNA polymerase III promoter.

    Depositor

    Marvin Wickens

    Article

    Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRS416-PTEF1-MCP-no_intron

    Plasmid

    #127597

    Purpose

    low-copy URA-selectable plasmid, 1xFLAG-MCP under TEF1 promoter

    Depositor

    David Bartel

    Article

    Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

    Insert

    1xFLAG-MCP expression cassette
    

    Expression

    Yeast

    Available Since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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