We narrowed to 10,900 results for: CHL

1. pLV U6-sgRNA UbC-GFP

   Plasmid

   #106948

   Purpose

   sgRNA cloning cassette using BsmBI/Esp3I enzymes with GFP marker

   Depositor

   Albert Edge

   Article

   Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

   Insert

   sgRNA BsmBI/Esp3I cloning site
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-CAG-LMO1 (GLuc-ChR2-EYFP)

   Plasmid

   #72888

   Purpose

   fusion protein of Gaussia luciferase, Channelrhodopsin-2, and enhanced yellow fluorescent protein (luminopsin) for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Berglund et al PLoS One. 2013;8(3):e59759. doi: 10.1371

   Insert

   Gaussia luciferase, Channelrhodopsin-2, EYFP
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUB-nSypHer (NLS:sypHer:NLS)

   Plasmid

   #84731

   Purpose

   Expresses sypHer in plant cells-biosensor for pH targeted to Nuclei

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

   Insert

   nSypHer
   

   Use

   Tags

   NLS (nuclear localisation signal and NLS (nuclear…

   Expression

   Plant

   Mutation

   Promoter

   ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

   Available since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBZcsx27

   Plasmid

   #89900

   Purpose

   Expresses csx27 from B. zoohelcum ATCC 43767 in bacteria. Expression driven from the Lac promoter

   Depositor

   Feng Zhang

   Article

   Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

   Insert

   csx27
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Lac

   Available since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV sgRNA-Notch1 #1 GFP

   Plasmid

   #106950

   Purpose

   Lentivirus encoding sgRNA targeting murine Notch1. Includes GFP marker.

   Depositor

   Albert Edge

   Article

   Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

   Insert

   gRNA targeting Notch1 (Notch1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWPXL-Hes1

   Plasmid

   #36983

   Depositor

   Bob Weinberg

   Article

   Guo et al Cell. 2012 Mar 2;148(5):1015-28.

   Insert

   Hes1 (Hes1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pH7WG2B-OsMIR390-B/c

   Plasmid

   #61465

   Purpose

   Plant expression vector (OsUBI promoter) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.

   Depositor

   James Carrington

   Article

   Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.1

   Insert

   OsMIR390-B/c
   

   Use

   Tags

   Expression

   Plant

   Mutation

   O. sativa MIR390 precursor sequence including a c…

   Promoter

   OsUBI

   Available since

   March 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. rAAV-CAG::FLEX-rev:ChR2HA:2a:PSAML141F,Y115F:GlyR

   Plasmid

   #32483

   Purpose

   Cre-dependent expression of ChR2 and PSAM-GlyR (L141F,Y115F) neuronal inhibitor, with IRES-EGFP marker

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

   Insert

   ChR2HA-2a-PSAML141F,Y115F:GlyR
   

   Use

   AAV and Cre/Lox; Adeno-associated virus, flex swi…

   Tags

   ChR2 2A

   Expression

   Mutation

   Promoter

   CAG

   Available since

   April 11, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBSFI-myr-akt-S473A

   Plasmid

   #49190

   Purpose

   cDNA of Akt1 with myristyolation signal and point mutation from S to A at position 473 in cloning vector

   Depositor

   Peter Vogt

   Article

   Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Adaptor plasmid

   Tags

   HA tag and myristolation signal

   Expression

   Mutation

   S473A

   Promoter

   T7

   Available since

   Dec. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pROF402

    Plasmid

    #155334

    Purpose

    Binary vector for expression of FLuc controlled by the blue light-repressible system 

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    LB_P35Senhancer(-953 to -51)-(C120)5-PhCMVmin-FLuc-T35S_RB
    

    Use

    Luciferase and Synthetic Biology; Eukaryotic expr…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pROF441

    Plasmid

    #155336

    Purpose

    Binary vector for expression of a gRNA targeting AtAP1 promoter 

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    LB_PAtU6-26-gRNA(PAtAP1)-sgRNA_RB
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pY005 (pcDNA3.1-hLb3Cpf1)

    Plasmid

    #69977

    Purpose

    Expresses humanized Lb3Cpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hLb3Cpf1
    

    Use

    CRISPR

    Tags

    NLS & 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pET28a-MSP1D1deltaH4/2H5

    Plasmid

    #71715

    Purpose

    Membrane scaffold protein (MSP), helix 4/2 + helix5 deletion

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Use

    Tags

    6xHis and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV hSyn1 ChR2 ET-TC 2A tDimer

    Plasmid

    #101361

    Purpose

    High efficiency channelrhodopsin, neuron specific promoter, red fluorescent protein

    Depositor

    Thomas Oertner

    Article

    Berndt et al Proc Natl Acad Sci U S A. 2011 May 3;108

    Inserts

    Channelrhodopsin-2
    synaptophysin-red fluorescent protein
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    E123T , T159C

    Promoter

    human Synapsin

    Available since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pXPR_BRD003 sgKEAP1-2

    Plasmid

    #74986

    Purpose

    sgKEAP1-2, puromycin selection

    Depositor

    William Hahn

    Article

    Krall et al Elife. 2017 Feb 1;6:e18970. doi: 10.7554

    Insert

    KEAP1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. JN202: pMVP/SB/Blast-DEST

    Plasmid

    #121864

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Blast selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-MSP1D1deltaH4/2

    Plasmid

    #71712

    Purpose

    Membrane scaffold protein (MSP), half of helix 4 truncated

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Use

    Tags

    6xHis and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBSFI-myr-akt-T308A

    Plasmid

    #49189

    Purpose

    cDNA of Akt1 with myristyolation signal and point mutation from T to A at position 308 in cloning vector

    Depositor

    Peter Vogt

    Article

    Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Adaptor plasmid

    Tags

    HA tag and myristolation signal

    Expression

    Mutation

    T308A

    Promoter

    T7

    Available since

    Dec. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pSKA397

    Plasmid

    #80380

    Purpose

    mini-Tn7 delivery plasmid with metE under the cpcG2Δ59 promoter.

    Depositor

    Mustafa Khammash

    Article

    Milias-Argeitis et al Nat Commun. 2016 Aug 26;7:12546. doi: 10

    Inserts

    cat
    metE
    

    Use

    Synthetic Biology; Mini-tn7 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    cat promoter and cpcG2Δ59

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSicoR-Ef1a-mCh-Puro-GFPi

    Plasmid

    #31848

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Insert

    GFP RNAi
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA; Ef1alpha for mCherry-puromycin

    Available since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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