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pENTR2b-Src(WT)

Plasmid

#215459

Purpose

Gateway entry vector with Src wild-type (WT)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Src (SRC Chicken)

Use

Gateway entry vector

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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ADEPT-pTarget-Msp4

Plasmid

#238037

Purpose

Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Promoter

lac

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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ADEPT-pTarget-Msp1

Plasmid

#238034

Purpose

Encodes sfGFPunder lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Promoter

lac

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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ADEPT-pTarget-Msp2

Plasmid

#238035

Purpose

Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Promoter

lac

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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ADEPT-pTarget-Msp3

Plasmid

#238036

Purpose

Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Promoter

lac

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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ADEPT-pTarget-Msp6

Plasmid

#238039

Purpose

Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Promoter

lac

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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ADEPT-pTarget-Msp5

Plasmid

#238038

Purpose

Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Promoter

lac

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO_24xGCN4_PCC_24xPP7

Plasmid

#236094

Purpose

Constitutively encodes 24xGCN4-PCC-24xPP7 stem loops, for visualization of the Pcc mRNA.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

24xGCN4-PCC-24xPP7

Expression

Mammalian

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO_24xGCN4_MUT_24xPP7

Plasmid

#236093

Purpose

Constitutively encodes 24xGCN4-MUT-24xPP7 stem loops, for visualization of the Mut mRNA.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

24xGCN4-MUT-24xPP7

Expression

Mammalian

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_GFP

Plasmid

#235611

Purpose

Encodes GFP. For use in transfections as a negative control without epitope tags.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

GFP

Expression

Mammalian

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_NES_V5_GFP

Plasmid

#235632

Purpose

Encodes V5-GFP with an N-terminal nuclear export sequence. Used as a no-ALIBi negative control.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

GFP

Tags

NES-V5

Expression

Mammalian

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pUB_SM-RLuc0---MS2

Plasmid

#210572

Purpose

Expresses SM(FLAG)-nonoptimal Renilla with 3'UTR MS2 hairpins

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-nonoptimal Renilla luciferase

Use

Lentiviral and Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Promoter

UBC human

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUB_SM-RLuc100---MS2

Plasmid

#210573

Purpose

Expresses SM(FLAG)-optimal Renilla with 3'UTR MS2 hairpins

Depositor

Olivia Rissland

Article

Barrington et al Cell Rep. 2023 Dec 26;42(12):113413. doi

Insert

Spaghetti monster (FLAG)-optimal Renilla luciferase

Use

Lentiviral and Luciferase

Tags

Spaghetti monster (FLAG)

Expression

Mammalian

Promoter

UBC human

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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lentiGuideSLC35C1-neo

Plasmid

#220868

Purpose

encodes CRISPR sgRNA targeting human SLC3C1

Depositor

Jennifer Kohler

Article

Ghorashi et al bioRxiv [Preprint]. 2023 Aug 3:2023.08.0

Insert

SLC35C1 (SLC35C1 Human)

Use

Lentiviral

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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TCT-Universal plasmid

Plasmid

#221852

Purpose

A universal plasmid for expressing any protein with TetraCysteine motif

Depositor

Artur Krężel

Article

Antonenko et al ACS Omega. 2024 Oct 30;9(45):45127-45137

Insert

Tetra Cysteine tag

Expression

Bacterial

Mutation

No

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pTarget-Dual

Plasmid

#207884

Purpose

Carries the cognate target sites for the homing endonucleases I-OnuI and I-GpeMI.

Depositor

David Edgell

Article

Foo et al Nucleic Acids Res. 2024 Feb 28;52(4):206

Inserts

I-OnuI target site
I-GpeMI target site

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-MLG-EGFP-LST2

Plasmid

#220785

Purpose

Expresses N-terminal EGFP-LST2 in mammalian cells

Depositor

Timm Maier

Article

Battaglioni et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

LST2 (ZFYVE28 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-MLG-HMF-LST2

Plasmid

#220786

Purpose

Expresses N-terminal His10-Myc-FLAG-LST2 in mammalian cells

Depositor

Timm Maier

Article

Battaglioni et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

LST2 (ZFYVE28 Human)

Tags

10xHis-Myc-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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STREP-SUMO-FLAG-SeKaiC

Plasmid

#218179

Purpose

WT SeKaiC for phosphorylation/dephosphorylation study

Depositor

Andy LiWang

Article

Kim et al Proc Natl Acad Sci U S A. 2008 Sep 2;105

Insert

STREP-SUMO-FLAG-SeKaiC

Tags

STREP-Tag

Expression

Bacterial

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX SYT9 C2A

Plasmid

#195707

Purpose

Bacterial expression plasmid encoding GST-tagged SYT9 C2A domain

Depositor

Edwin Chapman

Article

Seibert et al J Neurosci. 2023 Mar 1;43(9):1475-1491.

Insert

Syt9 (Syt5 Mouse)

Tags

GST

Expression

Bacterial

Mutation

encodes amino acids 124-211 of mouse SYT9

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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