We narrowed to 25,030 results for: Nes

1. pCAGGS/XBB.1.16-RBD

   Plasmid

   #213070

   Purpose

   Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.16 variant

   Depositor

   Priyamvada Acharya

   Article

   Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

   Insert

   pCAGGS/XBB.1.16-RBD (S )
   

   Use

   Tags

   6 His Tag

   Expression

   Mammalian

   Mutation

   G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

   Promoter

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-nSpCas9-APOBEC3A

   Plasmid

   #209040

   Purpose

   Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC3A is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   APOBEC3A-nSpCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   Tight TRE promoter

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAc-mCh-Tub

   Plasmid

   #24288

   Depositor

   Ron Vale

   Article

   Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

   Insert

   Alpha-Tubulin (alphaTub84B Fly)
   

   Use

   Tags

   mCherry

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   April 23, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pαH-S-GSAS-2P-Omicron-BA.5

   Plasmid

   #213828

   Purpose

   Mammalian cell expression of SARS-CoV-2 Spike protein of OMICRON.2P.BA5 variant

   Depositor

   Priyamvada Acharya

   Article

   Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

   Insert

   pαH-S-GSAS/Omicron-2P-BA5 (S )
   

   Use

   Tags

   HRV 3C cleavage site (before tags) 8X His tag 2X…

   Expression

   Mammalian

   Mutation

   G339D,R346T,S371F,S373P,S375F,T376A,D405N,R408S,K…

   Promoter

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a(+)-SpdCas9-SBiT_Strep-Tag-II

   Plasmid

   #198750

   Purpose

   Expresses S. Pyogenes dCas9 fused to SmallBiT fragment of split NanoLuc luciferase

   Depositor

   Maarten Merkx

   Article

   van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

   Insert

   SpdCas9 - SmallBiT
   

   Use

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   D10A, H840A (nuclease inactivating mutations in &…

   Promoter

   T7

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a(+)-SpdCas9-LBiT_Strep-Tag-II

   Plasmid

   #198751

   Purpose

   Expresses S. Pyogenes dCas9 fused to LargeBiT fragment of split NanoLuc luciferase

   Depositor

   Maarten Merkx

   Article

   van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

   Insert

   SpdCas9 - LargeBiT
   

   Use

   Tags

   Strep-tag II

   Expression

   Bacterial

   Mutation

   D10A, H840A (nuclease inactivating mutations in &…

   Promoter

   T7

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLV2-U6gRNA5(hYAP1-Y2B)-PGKpuro2AmCherry-W

   Plasmid

   #163179

   Purpose

   Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of mCherry tag

   Depositor

   Sok Ching Cheong

   Article

   Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

   Insert

   YAP1 (YAP1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKLV2-U6gRNA5(hWWTR1-W1K)-PGKpuro2AmCherry-W

   Plasmid

   #163176

   Purpose

   Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of mCherry tag

   Depositor

   Sok Ching Cheong

   Article

   Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

   Insert

   WWTR1 (WWTR1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS/XBB.1.5-RBD

   Plasmid

   #213038

   Purpose

   Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.5 variant

   Depositor

   Priyamvada Acharya

   Article

   Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

   Insert

   pCAGGS/XBB.1.5-RBD (S )
   

   Use

   Tags

   6 His Tag

   Expression

   Mammalian

   Mutation

   G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

   Promoter

   Available Since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pT2-shATRX

    Plasmid

    #124258

    Purpose

    Expresses shRNA targeting ATRX. Construct has inverted repeats to be used in Sleeping beauty system.

    Depositor

    Maria Castro

    Article

    Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

    Insert

    shATRX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBABE_hygro_mRFP1_NRF2(C506S, L4A)

    Plasmid

    #136580

    Purpose

    mammalian expression of mRFP1-tagged human NRF2(C506S, Leuzip mut)

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    NRF2 (C506S, Leuzip mut) (NFE2L2 Human)
    

    Use

    Retroviral

    Tags

    N-mRFP1-FLAG

    Expression

    Mammalian

    Mutation

    The C506S mutation (C513S in the actual sequence …

    Promoter

    Available Since

    July 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223-TSSK3

    Plasmid

    #23553

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    TSSK3 (TSSK3 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pKLV2-U6gRNA5(hWWTR1-W2B)-PGKpuro2AmCherry-W

    Plasmid

    #163177

    Purpose

    Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of mCherry tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

    Insert

    WWTR1 (WWTR1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAGGS-Omicron.BA.5-RBD

    Plasmid

    #213478

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.BA.5 variant

    Depositor

    Priyamvada Acharya

    Article

    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

    Insert

    pCAGGS/Omicron.BA.5 RBD (S )
    

    Use

    Tags

    6 His Tag

    Expression

    Mammalian

    Mutation

    G339D,R346T,S371F,S373P,S375F,T376A,D405N,R408S,K…

    Promoter

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAGGS/EG5-RBD

    Plasmid

    #213410

    Purpose

    Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.EG5 variant

    Depositor

    Priyamvada Acharya

    Article

    Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

    Insert

    pCAGGS/EG5-RBD (S )
    

    Use

    Tags

    6 His Tag

    Expression

    Mammalian

    Mutation

    G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

    Promoter

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKLV2-U6gRNA5(hYAP1-Y2B)-PGKpuro2ABFP-W

    Plasmid

    #163178

    Purpose

    Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of BFP tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

    Insert

    YAP1 (YAP1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    BFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLL3.7-EF-EYFP-YAP1_Y3F-PolyA

    Plasmid

    #112288

    Purpose

    Fluorescently labels human YAP1-2gamma isoform (504 aa) Y3F mutant _ tyrosines 341, 357 and 394 corresponding to tyrosines 391, 407 and 444 in this isoform

    Depositor

    Erik Sahai

    Article

    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

    Insert

    EYFP-YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    3 Tyrosines (341, 357 and 394 corresponding to ty…

    Promoter

    EF-1 alpha

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223-TSSK1

    Plasmid

    #23740

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    TSSK1 (TSSK1B Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pDONR223-TSSK2

    Plasmid

    #23397

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    TSSK2 (TSSK2 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    1MM

    Promoter

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pLenti-CAG-NOTCH2NL-delta EGF repeats (N2NL-delta EGF)-ires-EGFP

    Plasmid

    #122955

    Purpose

    Lentiviral expression of NOTCH2NL delta EGF repeats under CAG promoter with EGFP expression

    Depositor

    Pierre Vanderhaeghen

    Article

    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

    Insert

    NOTCH2NL-delta EGF repeats (NOTCH2NLB Human)
    

    Use

    Lentiviral

    Tags

    6xhis tag

    Expression

    Mutation

    deletion of EGF repeats from full length NOTCH2NLB

    Promoter

    CAG promoter

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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