Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 910 results for: Nes

Showing: 1 - 20 of 910 results

  1. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Fluorescent Proteins Empty Backbones Fluorescent Proteins: Empty Backbones Blue/UV Cyan Green Yellow Orange... present. Looking for more empty backbones? Visit our Empty Backbones page or search our complete collection...Explore empty plasmid backbones with different fluorescent tags to create fusion proteins with your gene...list many popular fluorescent proteins and empty backbones for expression and cloning of fusion proteins,...the help of Erik Snapp . Note for all tables: Brightness is the product of exctinction coefficient and...yield divided by 1,000. Note that the effective brightness of a fluorescent protein may vary depending on...Blue/UV Protein Excitation (nm) Emission (nm) Brightness pKa Maturation Structure Plasmids Sirius 355 ...

  2. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... Collections Empty Backbones Choosing Your Perfect Plasmid Backbone Empty backbones are plasmids containing...A selection of Addgene's empty vector backbones curated by species, epitope tags/fusion proteins, function...guide to a selection of Addgene's empty vector backbones. For the most part, we will assume that you want... Host Relevant Promoters Representative Empty Backbones Mammalian CMV, SV40, EF1a, CAG, Ubc Transient ...- Tet-inducible Find more Tet-inducible empty backbones on our Tetracycline (Tet) Inducible Expression...Hygromiycin resistance marker Also see more empty backbones on our Plant Plasmids and Resources collection...Protein Common uses Representative Empty Backbones Flag Epitope tag c-Flag pcDNA3 or FLAG-HA-pcDNA3.1...

  3. Brain Initiative Collection

    Type
    Collection
    ...the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons Yulong Li 123308-AAVrg...the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m in neurons Yulong Li 123309-AAV9...the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h in neurons Yulong Li 123309-AAVrg...the genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h in neurons Yulong Li 123310-AAV9...the genetically-encoded fluorescent norepinephrine(NE) control sensor GRAB_NEmut in neurons Yulong Li 123310...the genetically-encoded fluorescent norepinephrine(NE) control sensor GRAB_NEmut in neurons Yulong Li 125560...combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors...

  4. Retrograde AAV viral preps

    Type
    Collection
    ...Syn genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1m Norepinephrine sensor Yulong Li...Syn genetically-encoded fluorescent norepinephrine(NE) sensor GRAB_NE1h Norepinephrine sensor Yulong Li...control norepinephrine sensor (does not respond to NE) Norepinephrine sensor Yulong Li 20297 pAAV-EF1a-...serotype to deliver Cre-dependent transgenes in Cre transgenic lines. As part of our standard viral production...dTomato Calcium sensor Jonathan Ting 100853 pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 Syn Cre-dependent jRGECO1a...Calcium Sensor Douglas Kim , GENIE Project 100854 pAAV.Syn.NES-jRGECO1a.WPRE.SV40 Syn jRGECO1a Calcium Sensor...

  5. Zhang Lab CRISPR Page

    Type
    Collection
    ...knockout screening in human cells. Shalem O, Sanjana NE, Hartenian E, Shi X, Scott DA, Mikkelsen TS, Heckl...genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nat Methods . 2014 Aug;11(8):...contain a combination of CRISPR-associated (Cas) genes as well as non-coding RNA elements capable of programming...CRISPR-mediated nucleic acid cleavage. We have harnessed the type II CRISPR nuclease system to facilitate...implemented in mammalian cells by co-expressing the S. pyogenes Cas9 (SpCas9) nuclease along with the guide RNA...for the transcriptional activation of endogenous genes. It consists of three components: A nucleolytically..., Nature 2015). The dual vector system uses S. pyogenes Cas9 (SpCas9), using one vector to express SpCas9...

  6. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...pcDNA3.1-hSNCA-NE SNCA NE CMV Parkinson's Shu Leong Ho 102363 pcDNA3.1-hNurr1-NE NR4A2 NE CMV Parkinson's...pSIN-PAmCherry-mSNCA-NE SNCA NE EF-1 alpha Parkinson's Shu Leong Ho 102366 pSIN-hSNCA-NE SNCA NE EF-1 alpha Parkinson's...215486 FUW-hSNCA-NE SNCA NE Ubc Parkinson's Ophir Shalem 215487 FUW-hSNCA(D2R)-NE SNCA NE Ubc Parkinson'...Parkinson's Ophir Shalem 215488 FUW-hSNCA(D2E)-NE SNCA NE Ubc Parkinson's Ophir Shalem 215489 FUW-hSNCA-GFP SNCA...APP CMV Alzheimer's Helene Marie 107544 pAAV-AICD-NES-IRES-hrGFP APP V5 CMV Alzheimer's Helene Marie 107548...Alzheimer's, ALS Eran Hornstein 229082 PiggyBac-APEX-V5-NES TARDBP V5, APEX, BFP TRE3G ALS Eran Hornstein 229738...SFFV Parkinson's Denes Hnisz 145270 pHR-TBP(53Q)-IDR-mCherry-Cry2-NLS TBP Parkinson's Denes Hnisz 145283 ...

  7. Biosensor AAV Preps

    Type
    Collection
    ...SF-iGluSnFr Glucose Sensors iGlucoSnFr Norepinephrine (NE) Sensors GRAB_NE nLight Oxytocin (OT) Sensors GRAB_OT...iGlucoSnFr mRuby2 Constitutive 9 Looger Norepinephrine (NE) Sensors 123308 pAAV-hSyn-GRAB_NE1m Syn GRAB_NE1m... Griesbeck Calcium Sensor: jRCaMP1 100846 pAAV.CAG.Flex.NES-jRCaMP1a.WPRE.SV40 CAG jRCaMP1a none Cre dependent... dependent 1 Kim , GENIE 100848 pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 Syn jRCaMP1a none Constitutive 1, 9 Kim...Kim , GENIE 100849 pAAV.CAG.Flex.NES-jRCaMP1b.WPRE.SV40 CAG jRCaMP1b none Cre dependent 1 Kim , GENIE ...GENIE 100850 pAAV.Syn.Flex.NES-jRCaMP1b.WPRE.SV40 Syn jRCaMP1b none Cre dependent 1 Kim , GENIE 100851 pAAV.Syn.NES-jRCaMP1b.WPRE.SV40...CaMPARI none Constitutive 1, 5, 9 Looger 101060 pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40 Syn CaMPARI2 his Constitutive...

  8. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...AID) enzyme. AID, however, only changes cytidines or guanines to the other three bases, which limits the...directed mutagenesis to generate specific mutations in your plasmids Use REPLACER Mutagenesis to mutate...Mutagenesis is a tool that both evolution and molecular biologists use to tinker with DNA. Making changes...evolution of new phenotypes, and forward genetics harnesses this at a large scale by screening diverse libraries...region. EvolvR, a CRISPR-Cas9 based targeted mutagenesis method developed by the Dueber Lab at Berkeley...potential applications. Challenges of existing mutagenesis methods Several continuous diversification methods...error prone polymerase I (PolI3M) is capable of mutagenesis in E. Coli, but this approach requires special...

  9. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...practical due to the low intensity of luminescent proteins. To make luminescent imaging a reality, Addgene depositor...with Venus. This luminescent technology is based on the principle of bioluminescence resonance energy ...reporter genes for endogenous protein fusions created using CRISPR/Cas9. eNLs offer high luminescent signal... a few drawbacks associated with luminescent imaging: A luminescent substrate is required for imaging...Researchers have long been interested in using luminescence to get around these issues, but this solution...fusion proteins. Solving the problems of low luminescence By itself, Renilla luciferase has a low quantum... hasn’t stopped some scientists from using chemiluminescent proteins for imaging, but it has precluded...

  10. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ... the mutagenesis reaction. Alternative approaches to site directed mutagenesis Point-mutagenesis is fairly...Laursen from Cornell University. Site directed mutagenesis is a highly versatile technique that can be used...analysis of proteins (to perform alanine scanning mutagenesis or targeted substitution of key residues), and...relevant in this age of CRISPR; site-directed mutagenesis generally applies to plasmids, but may also facilitate...plasmid contains the template, site-directed mutagenesis can be used to mutate the PAM sequence (an NGG...Cas9 induced cleavage. Summary of Site Directed Mutagenesis In brief, point-mutations can be introduced to...the desired modification. Finally, the positive clones are sequenced to confirm the desired modification...

  11. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nature Methods. 2014 Aug;11(8...Protocols Generating Stable Cell Lines with Lentivirus Generating Stable Cell Lines with Lentivirus You may also...Protocol to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector...This protocol can be used to generate stable cell lines expressing a gene of interest from an integrated...transient transfection approaches, generating cell lines using lentiviral vectors enables long-term protein...293T cells but can be adapted to alternative cell lines. Workflow Timeline Day 0: Seed and transduce Cells...needed Day 14–18: Expand and harvest stable cell lines Equipment Class II, Type A2 Biological Safety Cabinet...

  12. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...expression of surrounding genes. However, there are ways to create scarless mutations, ones that don’t leave any...toxins for counter-selection In 2015, the Chen lab harnessed a group of bacterial toxins to facilitate efficient...Red that contained one of eight bacterial toxin genes isolated from E. coli or Psuedomonas aeruginosa....toxins, and phage holins. They hypothesized that genes that had evolved specifically for toxicity would... pathogenic Proteobacteria. We placed the toxin genes under control of a rhamnose-inducible promoter, ... they are just spontaneous antibiotic resistant clones. But if they are blue or magenta, they are real...

  13. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    ...target-specific ones to application-specific ones to anything goes. We've shared the ones we're familiar...search engines and antibody data repositories to find an antibody for your experiment. Search engines let...which share validation data, and antibody search engines, which allow you to search many different antibody...Table 1: Antibody data repositories and search engines Website Targets Application Purpose ...

  14. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...allowed scientists to tag and light up endogenous genes of interest to better understand normal protein ...vectors. These vectors can be used to tag endogenous genes and simultaneously introduce a selection marker ...vectors can be easily modified to tag hundreds of genes, generating powerful genetic screening libraries...easily create C-terminal tag fusions of endogenous genes in human cells. To use CRISPaint, users need 3 vectors... Learn more about these constructs and the cell lines they’ve been used to create in the Allen Institute...

  15. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...would spend hours to days trying find their correct clones. Additionally, the presence of a plasmid is disadvantageous...Historically, antibiotics have also been used to disrupt genes at the chromosomal level. Scientists introduce an...

  17. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...Healthcare consulting firms that specialize in business strategy tend to be mostly engaged by pharma and...companies. Medical Affairs groups work on building awareness among physicians and scientists about clinical...collaborative, and financial functions associated with the business. Commercial teams routinely work with consulting... a very short amount of time. Such a decision determines whether the client invests substantial monetary...consultants are usually trained in the life sciences, in business, in healthcare economics, or in some combination... you, you’ll have the opportunity to learn the business side of consulting cases, while feeling like you...healthcare space that can benefit from the blend of business and scientific understanding of successful therapeutics...

  18. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    ...assembly methods. A new method, REPLACR-mutagenesis, harnesses the power of bacterial recombineering to...REPLACR-mutagenesis could save busy scientists a lot of time. Let us know how REPLACR-mutagenesis works ...Comparison of REPLACR-mutagenesis with other common methods for site directed mutagenesis   References 1. ...al. (2016). “REPLACR-mutagenesis: a one-step method for site-directed mutagenesis by recombineering.” ...Site-directed mutagenesis (SDM) is one of the key tools researchers use to prove causation in molecular... Plasmids After PCR). Current site directed mutagenesis methods Current SDM methods rely on in vitro ...structure or too much sequence similarity. REPLACR-mutagenesis: Letting bacteria do the work If you’ve conducted...

  20. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...yeast gene of interest. These fluorescently tagged genes are expressed under native conditions and allow ...looking at their performance in categories such as brightness, stability, and disruption of the tagged protein...be interested in attaching epitope tags to their genes of interest, allowing for easy capture and detection...
Showing: 1 - 20 of 910 results