We narrowed to 5,627 results for: mcs
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Plasmid#236447Purposeinsect expression of E2F2 for protein purificationDepositorInsertE2F2 (E2F2 Human)
ExpressionInsectAvailable SinceMay 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
p70.unipA.NGFR.mCMV.PGK.mBFP2.p27K.WPRE
Plasmid#228766Purposeinsert a blue fluorescent protein mBFP2, a cyclin-dependent kinase inhibitor p27KDepositorAvailable SinceMay 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pk335-CARGO-6mer-35kb-DSF-1-6
Plasmid#227487Purpose6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locusDepositorInsertCARGO to 35kb Downstream Fgf5
UseCRISPRAvailable SinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pk335-CARGO-6mer-35kb-DSF-6-11
Plasmid#227488Purpose6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locusDepositorInsertCARGO to 35kb Downstream Fgf5
UseCRISPRAvailable SinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pk335-CARGO-11mer-35kb-DSF-1-11
Plasmid#227489Purpose11-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locusDepositorInsertCARGO to 35kb Downstream Fgf5
UseCRISPRAvailable SinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pk335-CARGO-6mer-14kb-USF-1-6
Plasmid#227470Purpose6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locusDepositorInsertCARGO to 14kb Upstream Fgf5
UseCRISPRAvailable SinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pk335-CARGO-6mer-14kb-USF-7-12
Plasmid#227471Purpose6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locusDepositorInsertCARGO to 14kb Upstream Fgf5
UseCRISPRAvailable SinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pk335-CARGO-12mer-14kb-USF-1-12
Plasmid#227472Purpose12-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locusDepositorInsertCARGO to 14kb Upstream Fgf5
UseCRISPRAvailable SinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW371 (PB) (ZNF35/ZNF35tar-6bp spacers)x4-H2B-BFP insul CMV-TO-ZNF35(5-8)-ZNF35(5-8)-deimlink-NZF
Plasmid#236173PurposePiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/ZNF35-driven BFP, and ZNF35/ZNF35-NZF transcription factorDepositorInsertsTagsmTagBFP2ExpressionMammalianPromoterCMV and four ZNF35/ZNF35 sitesAvailable SinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVEA3-KNC
Plasmid#234801PurposeMammalian expression of bovine arrestin-3 (K11A, K12A, L49A, D51A, R52A, L69A, Y239A, D241A, C252A, P253A, D260A, Q262A)DepositorInsertArrestin-3 (ARR3 Bovine)
TagsVenusExpressionMammalianMutationK11A, K12A, L49A, D51A, R52A, L69A, Y239A, D241A,…Available SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW482 CMV-TO-ADAR2dd(E488Q, T501A)-PUF-9R-MARIAcomp(V241D, H243M) [D241C, M243Q] (FLP-IN)
Plasmid#236128PurposePlasmid encoding ADAR2dd attached to PUF-9R with deimmunizing and function-restoring mutations under control of CMV promoter with two TetR binding sitesDepositorInsertADAR2dd-PUF-9R (ADARB1 Human, Synthetic)
ExpressionMammalianMutationE488Q, T501A in ADAR2dd; V241C, H243Q in PUF-9RPromoterCMV promoter with two TetR binding sitesAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW370 (PB) (ZNF35/ZNF250tarv3-6bp spacers)x4-H2B-BFP insul CMV-TO-ZNF35(5-8)-ZNF250(1-4)-deimlink-NZF
Plasmid#236172PurposePiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/ZNF250-driven BFP, and ZNF35/ZNF250-NZF transcription factorDepositorInsertsTagsmTagBFP2ExpressionMammalianPromoterCMV and four ZNF35/ZNF250 sitesAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW489 CMV-TO-ZNF35(5-8)-ZNF250(1-4)-(GGS)x8[G2V G4W S6L G7L G10W G11D G13L G17M G19I G22M G23Q S24Q]-FKBP (FLP-IN)
Plasmid#236147PurposePlasmid encoding the ZNF35/ZNF250 zinc finger array attached by a deimmunized linker to FKBP1A, under control of CMV promoter with two TetR binding sitesDepositorInsertZNF35(5-8)-ZNF250(1-4)-deImmunLink-FKBP
ExpressionMammalianPromoterCMV promoter with two TetR binding sitesAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW267 CMV-TO-ADAR2dd(E488Q, T501A)-PUF-9R-MARIAcomp(V241D, H243M) (FLP-IN)
Plasmid#236127PurposePlasmid encoding ADAR2dd attached to PUF-9R with deimmunizing mutations under control of CMV promoter with two TetR binding sitesDepositorInsertADAR2dd-PUF-9R (ADARB1 Human, Synthetic)
ExpressionMammalianMutationE488Q, T501A in ADAR2dd; V241D, H243M in PUF-9RPromoterCMV promoter with two TetR binding sitesAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW372 (PB) (ZNF35/IKZF1tar-6bp spacers)x4-H2B-BFP insul CMV-TO-ZNF35(5-8)-IKZF1(2-4)-deimlink-NZF
Plasmid#236174PurposePiggyBac-integrated plasmid containing hygromycin resistance gene, ZNF35/IKZF1-driven BFP, and ZNF35/IKZF1-NZF transcription factor (with the IKZF1 portion containing ZFs 2-4 from that protein)DepositorInsertsTagsmTagBFP2ExpressionMammalianPromoterCMV and four ZNF35/IKZF1 sitesAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLAS2w.Phyg-CHST8 (3A)
Plasmid#232651Purposeexpresses CHST8 REP-to-AAA mutant in mammalian cellsDepositorInsertCHST8 (3A) (CHST8 Human)
UseLentiviralTagsFlagExpressionMammalianMutationR258A, E259A, P260APromoterCMVAvailable SinceMarch 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXJ-CD274-Nluc
Plasmid#232658PurposeNluc-tagged PD-L1 expresion in mammalian cells; lectin-captured luciferase reporter assayDepositorAvailable SinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXJ-Nluc-_(PLRSKK)-CA1-19
Plasmid#232657PurposeNluc-tagged modified CG_ in mammalian cells; lectin-captured luciferase reporter assayDepositorAvailable SinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCM100_ PB5'IR-hU6-gRNA-CS1- CAG-tdTomato- PB3'IR
Plasmid#229995PurposePiggyBac sgRNA cloning plasmid with tdTomato reporter with a capture sequence (cs1)DepositorTypeEmpty backboneUseCRISPRTagsNotI site for NEBuilder HiFi DNA Assembly with ss…ExpressionMammalianPromoterCAGAvailable SinceFeb. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MKI-MUT-P2A-GFP
Plasmid#229406PurposeLentiviral or overpexression of cDNADepositorInsertMKI-MUT-P2A-GFP
UseLentiviralTagsFLAGExpressionMammalianMutationinteraction mutant L109G; L143G (Control mutant)PromoterEFSAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only