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We narrowed to 48 results for: mcs

Showing: 1 - 20 of 48 results

  1. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    .... Scientists engineered a multiple cloning site (MCS) into the α-peptide (represented as an orange wedge...goes to plan and your DNA (red) is cloned into this MCS, the α-peptide gets interrupted as shown in cell ...plasmid (i.e. contains the α-complementation cloning MCS): pGEM-T, pUC18 and pUC19, and pBluescript are a ...the cell. Cloning fragments are inserted into an MCS in the center of this gene, disrupting the lethality...

  2. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...plasmid-containing bacteria. Multiple Cloning Site (MCS) Short segment of DNA which contains several restriction...easy insertion of DNA. In expression plasmids, the MCS is often downstream from a promoter. Insert Gene..., promoter or other DNA fragment cloned into the MCS for further study. Promoter Region Drives transcription...

  3. MXS Chaining

    Type
    Blog Post
    ... each flanked by the same multiple cloning site (MCS). Modules are assembled one at a time by standard...ligation. Upon ligation, the original pattern of the MCS is regenerated at the flanks of each ligation product...the method. Note that the restriction sites of the MCS are only regenerated at the 5’ and 3’ termini of ... the 5’ or 3’ terminus using the newly generated MCS. MXS-chaining also has the advantage of being directional...

  4. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ... al., 2014). Replaced the pDS132 MCS with a versatile, synthetic MCS (designed with the help of the DNA...from IGEM) upstream of the MCS to reduce transcriptional readthrough into the MCS to facilitate the cloning...

  5. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    ... modular structure. Two multiple cloning sites (MCS's) flank the M24 promoter, allowing it to be removed...removed and replaced if needed. In total, three MCS regions permit multiple regulatory elements to be inserted...

  6. Plasmid Cloning by PCR

    Type
    Blog Post
    ...recipient plasmid (usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid Bonus...orientation in the recipient plasmid by viewing the MCS and fusing the upstream restriction site to the forward...

  7. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Proximity-dependent biotin identification (BioID) MCS-BioID2-HA or myc-BioID2-MCS - To fuse your protein of interest ...tags or fusion proteins, a multiple cloning site (MCS), an inducible promoter, and/or a selectable marker...mCherry - Expresses mCherry tag in mammalian cells pBI-MCS-EGFP - Tet-inducible Find more Tet-inducible empty...their effects on protein production AAV-GfaABC1D-MCS--4x6T-WPRE - For astrocyte-selective expression in...Expresses mCherry tagged fusion proteins in worms ins:MCS; cryaa:RFP - A zebrafish insulin promoter vector...

  9. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

    Type
    Protocol
    ...Modify your plasmid MCS or add short sequences using oligo overlap cloning....plasmid, such as: Changing a Multiple Cloning Site (MCS) Adding short tags - ex: HA or Flag Cloning shRNAs... discuss how to add new restriction sites to the MCS of an empty vector. However, the following technique...that your favorite vector has a relatively limited MCS (BamHI - EcoRI - SalI) and you want to expand that...

  18. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...recipient plasmid (usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid Will result...restriction sites compatible with the recipient plasmid's MCS. However, you still need to avoid restriction enzymes...sites to your recipient plasmid : You can modify the MCS of your recipient plasmid using Annealed-oligo Cloning...

  20. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...fragment is inserted in a multiple cloning site (MCS) of an attL-containing entry vector.   Figure...
Showing: 1 - 20 of 48 results