We narrowed to 4 results for: mcs

1. Plasmid Modification by Annealed Oligo Cloning (with Protocols)

   Type

   Protocol

   ...Modify your plasmid MCS or add short sequences using oligo overlap cloning....plasmid, such as: Changing a Multiple Cloning Site (MCS) Adding short tags - ex: HA or Flag Cloning shRNAs... discuss how to add new restriction sites to the MCS of an empty vector. However, the following technique...that your favorite vector has a relatively limited MCS (BamHI - EcoRI - SalI) and you want to expand that...

2. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

   Type

   Protocol

   ...recipient plasmid (usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid Will result...restriction sites compatible with the recipient plasmid's MCS. However, you still need to avoid restriction enzymes...sites to your recipient plasmid : You can modify the MCS of your recipient plasmid using Annealed-oligo Cloning...

3. Plasmid Cloning by PCR (with Protocols)

   Type

   Protocol

   ...recipient plasmid (usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid. Bonus...orientation in the recipient plasmid by viewing the MCS and fusing the upstream restriction site to the forward...

4. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

   Type

   Protocol

   ...enzyme that cuts your vector's multiple cloning site (MCS), but does not cut your insert, you could try using...