We narrowed to 31 results for: mcs

.... Scientists engineered a multiple cloning site (MCS) into the α-peptide (represented as an orange wedge...goes to plan and your DNA (red) is cloned into this MCS, the α-peptide gets interrupted as shown in cell ...plasmid (i.e. contains the α-complementation cloning MCS): pGEM-T, pUC18 and pUC19, and pBluescript are a ...the cell. Cloning fragments are inserted into an MCS in the center of this gene, disrupting the lethality...

...plasmid-containing bacteria. Multiple Cloning Site (MCS) Short segment of DNA which contains several restriction...easy insertion of DNA. In expression plasmids, the MCS is often downstream from a promoter. Insert Gene..., promoter or other DNA fragment cloned into the MCS for further study. Promoter Region Drives transcription...

... each flanked by the same multiple cloning site (MCS). Modules are assembled one at a time by standard...ligation. Upon ligation, the original pattern of the MCS is regenerated at the flanks of each ligation product...the method. Note that the restriction sites of the MCS are only regenerated at the 5’ and 3’ termini of ... the 5’ or 3’ terminus using the newly generated MCS. MXS-chaining also has the advantage of being directional...

... al., 2014). Replaced the pDS132 MCS with a versatile, synthetic MCS (designed with the help of the DNA...from IGEM) upstream of the MCS to reduce transcriptional readthrough into the MCS to facilitate the cloning...

... modular structure. Two multiple cloning sites (MCS's) flank the M24 promoter, allowing it to be removed...removed and replaced if needed. In total, three MCS regions permit multiple regulatory elements to be inserted...

...recipient plasmid (usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid Bonus...orientation in the recipient plasmid by viewing the MCS and fusing the upstream restriction site to the forward...

... 2013 Oct 11;342(6155):253-257. PubMed. 3. Canver MC, Bauer DE, Dass A, Yien YY, Chung J, Masuda T, et...

...BM, Feldman J, Pavlovic MN, Gregory DJ, Poznansky MC, Sigal A, Schmidt AG, Iafrate AJ, Naranbhai V, Balazs...

...that will sequence over the Multiple Cloning Site (MCS) and into your insert. Check out our Vector Database...

....02.007 Moroz-Omori, E., Satyapertiwi, D., Ramel, MC., et al. Photoswitchable gRNAs for Spatiotemporally...

...BM, Swarts S, Mueller BM, van der Geer P, Manning MC, Fitchmun MI. Protein instability following transport...

...backbone. For example: pLXSN has the wild type LTR, an MCS for cloning X gene, an SV40 promoter, and Neomycin...

...Whitehead EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann M, Weissman JS. Cell. 2014 Oct 23...

... Harteveld Z, Aschenbrenner S, Bietz A, Waldhauer MC, Börner K, Fakhiri J, Schmelas C, Dietz L, Grimm ...anie.201606123  Kim JH, Rege M, Valeri J, Dunagin MC, Metzger A, Titus KR, Gilgenast TG, Gong W, Beagan...

...pKIL19, contained the ccdB gene in frame with an MCS. E. coli that were transformed with the empty vectors...

...Laboratory Publications, New York. Helma J, Cardoso MC, Muyldermans S, Leonhardt H (2015) Nanobodies and...

...fragment is inserted in a multiple cloning site (MCS) of an attL-containing entry vector.   Figure...

...Retroviral pMSCV-pBabeMCS-IRES-RFP IRES Retroviral pMSCV-IRES-YFP II IRES Retroviral pCMMP-MCS-IRES-Puro ...

.../doi.org/10.1093/nar/gky164 Cancellieri S, Canver MC, Bombieri N, Giugno R, Pinello L. CRISPRitz: rapid...

...Whitehead EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann M, Weissman JS (2014) Genome-Scale...