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We narrowed to 31 results for: mcs

Showing: 1 - 20 of 31 results

  1. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    .... Scientists engineered a multiple cloning site (MCS) into the α-peptide (represented as an orange wedge...goes to plan and your DNA (red) is cloned into this MCS, the α-peptide gets interrupted as shown in cell ...plasmid (i.e. contains the α-complementation cloning MCS): pGEM-T, pUC18 and pUC19, and pBluescript are a ...the cell. Cloning fragments are inserted into an MCS in the center of this gene, disrupting the lethality...

  2. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...plasmid-containing bacteria. Multiple Cloning Site (MCS) Short segment of DNA which contains several restriction...easy insertion of DNA. In expression plasmids, the MCS is often downstream from a promoter. Insert Gene..., promoter or other DNA fragment cloned into the MCS for further study. Promoter Region Drives transcription...

  3. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ... each flanked by the same multiple cloning site (MCS). Modules are assembled one at a time by standard...ligation. Upon ligation, the original pattern of the MCS is regenerated at the flanks of each ligation product...the method. Note that the restriction sites of the MCS are only regenerated at the 5’ and 3’ termini of ... the 5’ or 3’ terminus using the newly generated MCS. MXS-chaining also has the advantage of being directional...

  4. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    Published
    Aug. 15, 2019, 12:30 p.m.
    ... al., 2014). Replaced the pDS132 MCS with a versatile, synthetic MCS (designed with the help of the DNA...from IGEM) upstream of the MCS to reduce transcriptional readthrough into the MCS to facilitate the cloning...

  5. pSiM24: Simplifying Plant Genetic Engineering

    Type
    Blog Post
    Published
    Sept. 29, 2015, 2:30 p.m.
    ... modular structure. Two multiple cloning sites (MCS's) flank the M24 promoter, allowing it to be removed...removed and replaced if needed. In total, three MCS regions permit multiple regulatory elements to be inserted...

  6. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...recipient plasmid (usually in the Multiple Cloning Site (MCS)), but do not cut elsewhere on the plasmid Bonus...orientation in the recipient plasmid by viewing the MCS and fusing the upstream restriction site to the forward...

  13. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ... Harteveld Z, Aschenbrenner S, Bietz A, Waldhauer MC, Börner K, Fakhiri J, Schmelas C, Dietz L, Grimm ...anie.201606123  Kim JH, Rege M, Valeri J, Dunagin MC, Metzger A, Titus KR, Gilgenast TG, Gong W, Beagan...

  17. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...fragment is inserted in a multiple cloning site (MCS) of an attL-containing entry vector.   Figure...

  18. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...Retroviral pMSCV-pBabeMCS-IRES-RFP IRES Retroviral pMSCV-IRES-YFP II IRES Retroviral pCMMP-MCS-IRES-Puro ...

  19. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    .../doi.org/10.1093/nar/gky164 Cancellieri S, Canver MC, Bombieri N, Giugno R, Pinello L. CRISPRitz: rapid...

  20. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...Whitehead EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann M, Weissman JS (2014) Genome-Scale...
Showing: 1 - 20 of 31 results