We narrowed to 3,532 results for: mpo

1. NST1_pOpOn2.1

   Plasmid

   #228065

   Purpose

   Expresses NST1 in different cell types in Arabidopsis thaliana upon induction with dexamethasone.

   Depositor

   Charles Anderson

   Article

   Tamadaddi et al Ann Bot. 2024 Aug 30:mcae153. doi: 10.10

   Insert

   NAC SECONDARY WALL THICKENING PROMOTING FACTOR1, NST1 (NST1 Mustard Weed)
   

   Use

   Dexamethasone inducible vector

   Expression

   Plant

   Promoter

   CaMV35S for LhGR and pOp6-minimal CaMV35S for NST1

   Available Since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV-FLAG-TAOK2

   Plasmid

   #197862

   Purpose

   Expression of FLAG-tagged TAOK2 / PSK1 alpha in mammalian cells

   Depositor

   Jonathan Morris

   Article

   Koo et al Mol Cancer Ther. 2017 Nov;16(11):2410-24

   Insert

   TAOK2 (TAOK2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Wild Type

   Promoter

   SV40

   Available Since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNK2657

   Plasmid

   #219741

   Purpose

   MoClo-compatible Level 0 promoterless vector encoding hispidin-synthase from Mycena citricolor codon-optimised for expression in Nicotiana benthamiana, Pichia pastoris, Homo sapiens

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   hispidin- synthase from Mycena citricolor
   

   Use

   Synthetic Biology

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRXIP-GFP-CERT(PHD)(W33A)

   Plasmid

   #221761

   Purpose

   Stable expression of GFP-CERT(PHD)(W33A) in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   CERT (CERT1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   amino acids 1–116, mutated 33W to 33A

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCOLADuet-1_nsp10 (SARS-CoV-2)

   Plasmid

   #169158

   Purpose

   To express SARS-CoV-2 nsp10 in E. coli

   Depositor

   John Diffley

   Article

   Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

   Insert

   nsp10 (ORF1ab SARS-CoV-2)
   

   Tags

   Methionine

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T7

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. BcLOV4 fusion tools screening plasmid #2

   Plasmid

   #174512

   Purpose

   Cloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP in a pcDNA3.1 backbone.

   Depositor

   Brian Chow

   Article

   Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

   Insert

   plasmid B [BamHI]-mCherry-[XhoI]-GGGSx2-BcLOV4-[EcoRI]-GGGS-3xFLAG-STOP
   

   Tags

   3xFLAG and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNK115

   Plasmid

   #219745

   Purpose

   MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 codon-optimised for expression in Nicotiana benthamiana

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   mutant of fungal luciferase
   

   Use

   Luciferase and Synthetic Biology

   Mutation

   I3S, N4T, F11L, I63T, T99P, T192S, A199P

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNK116

   Plasmid

   #219742

   Purpose

   MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Nicotiana benthamiana

   Depositor

   Alexander Mishin

   Article

   Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

   Insert

   mutant of fungal hispidin-3-hydroxylase
   

   Use

   Synthetic Biology

   Mutation

   D37E, V181I, S323M, M385K

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLNCX2-OptoTGFBRs

   Plasmid

   #118965

   Purpose

   Retroviral expression OptoTGFBRs for transient or stable constitutive expression

   Depositor

   Won Do Heo

   Article

   Li et al ACS Synth Biol. 2018 Feb 16;7(2):443-451

   Insert

   OptoTGFBRs (Tgfb1, Tgfbr2 Mustard Weed, Mouse)
   

   Use

   Retroviral

   Tags

   tdTomato

   Promoter

   CMV

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. mGLDC

    Plasmid

    #72881

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Kim et al Nature. 2015 Apr 16;520(7547):363-7. doi

    Insert

    Gldc (Gldc Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pCAG-mDrc7-FLAG

    Plasmid

    #176470

    Purpose

    Expression vector of mouse dynein regulatory complex subunit 7 (Drc7) tagged with FLAG at C-terminus.

    Depositor

    Masahito Ikawa

    Article

    Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

    Insert

    dynein regulatory complex subunit 7 (Drc7 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1_Tau Authentic Alu 10-12V337M

    Plasmid

    #194169

    Purpose

    Insert: intronic Tau authentic Alu and repeat elements. Introns are flanked by the tau cDNA exons 10-12 with FTLD-Tau V337M mutant. Expresses tau circular RNA 12-->10 VM with 3X flag tag in exon 10.

    Depositor

    Stefan Stamm

    Article

    Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

    Insert

    Microtubule-associated protein tau 10-12 V337M
    

    Tags

    3X flag

    Expression

    Mammalian

    Mutation

    Changed Valine 337 to Methinonine

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. HT115_pAAV_hSyn-DiO-SomQuasAr6b_EGFP-P2A-somCheRiff_HA

    Plasmid

    #178827

    Purpose

    Cre-on bicistronic expression of QuasAr6b-based Optopatch under an neuronal promoter

    Depositor

    Adam Cohen

    Article

    Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

    Insert

    SomQuasAr6a_EGFP-P2A-somCheRiff_HA
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Tags

    EGFP, HA

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMXS-IRES-Blast POLD1

    Plasmid

    #160805

    Purpose

    Express POLD1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    POLD1 (POLD1 Human)
    

    Use

    Retroviral

    Mutation

    Codon optimized C-terminal 230 amino acids

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBMN-puro-P2A-FRB-Fis1

    Plasmid

    #135297

    Purpose

    stably express FBR-Fis1 in mammalian cells with puromycin selection

    Depositor

    Richard Youle

    Article

    Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

    Insert

    FRB-Fis1 (FIS1 Human)
    

    Use

    Retroviral

    Tags

    FRB

    Expression

    Mammalian

    Mutation

    Fis1 tail (aa 93-152)

    Available Since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. phBG-P53DD

    Plasmid

    #149707

    Purpose

    In vitro transcription of dominant negative form of P53 mRNA for RNA transfection into mammalian cells

    Depositor

    Erika Sasaki

    Article

    Watanabe et al Genes Cells. 2019 Jul;24(7):473-484. doi

    Insert

    Trp53 (Trp53 Mouse)
    

    Use

    Rna transcription

    Mutation

    deleted amino acid 14-301

    Promoter

    T7

    Available Since

    June 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-OptoFGFR1-Y766F

    Plasmid

    #59777

    Purpose

    Expresses optoFGFR1 with mutation in PLC-gamma binding phosphotyrosine site of FGFR1

    Depositor

    Won Do Heo

    Article

    Kim et al Chem Biol. 2014 Jul 17;21(7):903-12. doi

    Insert

    OptoFGFR1-Y766F (FGFR1 Mustard Weed, Human)
    

    Tags

    mCitrine

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-Hyg-Snrnp40-CRISPR-resistant

    Plasmid

    #134251

    Purpose

    Lentivector encoding CRISPR-resistant Snrnp40

    Depositor

    Bruce Beutler

    Article

    Zhang et al Nat Immunol. 2019 Oct;20(10):1322-1334.

    Insert

    Snrp40 (Snrnp40 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    mutated coding sequence “gataactatgcgacgttgaa” to…

    Promoter

    EF1a

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBIG1a_3xFlag-6His-nsp15 (SARS-CoV-2)

    Plasmid

    #169166

    Purpose

    Baculoviral transfer vector for the expression of SARS-CoV-2 nsp15 in insect cells

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2465-2479

    Insert

    3xFlag-6His-nsp15 (ORF1ab SARS-CoV-2, Synthetic)
    

    Tags

    3xFlag-6His

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. J7AAV-HDR Fah.2

    Plasmid

    #73451

    Purpose

    J7AAV-HDR U6sgFah.2 template2 to repair Fah mutation

    Depositor

    Wen Xue

    Article

    Yin et al Nat Biotechnol. 2016 Feb 1. doi: 10.1038

    Insert

    Fah (Fah Mouse)
    

    Use

    AAV

    Available Since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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