We narrowed to 10,268 results for: codon optimized

1. pCAG-hAsCas12a-RVR(S542R/K548V/N552R)-NLS(nucleoplasmin)-3xHA (AAS1065)

   Plasmid

   #114092

   Purpose

   CAG promoter expression plasmid for human codon optimized AsCas12a-RVR(S542R/K548V/N552R) nuclease with C-terminal NLS and HA tag

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized AsCas12a (S542R/K548V/N552R)
   

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Mutation

   S542R, K548V and N552R

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-hAsCas12a-RVR(S542R/K548V/N552R)-NLS(nucleoplasmin)-3xHA (AAS1065)

   Plasmid

   #114092

   Purpose

   CAG promoter expression plasmid for human codon optimized AsCas12a-RVR(S542R/K548V/N552R) nuclease with C-terminal NLS and HA tag

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized AsCas12a (S542R/K548V/N552R)
   

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Mutation

   S542R, K548V and N552R

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)-3xHA (AAS826)

   Plasmid

   #114091

   Purpose

   CAG promoter expression plasmid for human codon optimized AsCas12a(E174R/S542R) nuclease with C-terminal NLS and HA tag

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized AsCas12a (E174R/S542R)
   

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Mutation

   E174R and S542R

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAG-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)-3xHA (AAS826)

   Plasmid

   #114091

   Purpose

   CAG promoter expression plasmid for human codon optimized AsCas12a(E174R/S542R) nuclease with C-terminal NLS and HA tag

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized AsCas12a (E174R/S542R)
   

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Mutation

   E174R and S542R

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCJ199

   Plasmid

   #162672

   Purpose

   pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with C-terminal His tag, codon optimized for expression in E. coli K12.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   Putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with signal peptide
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   codon optimized for expression in E. coli K12

   Promoter

   T7/lac

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCJ199

   Plasmid

   #162672

   Purpose

   pET-21b(+) based plasmid for expression of the putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with C-terminal His tag, codon optimized for expression in E. coli K12.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   Putative MHET hydrolase from Comamonas thiooxydans (Genbank WP_080747404.1) with signal peptide
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   codon optimized for expression in E. coli K12

   Promoter

   T7/lac

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET11d-FHis-DND1-Y102A

   Plasmid

   #70082

   Purpose

   Bacterial expression of FLAG-and 6xHis-tagged human DND1 protein with defective RRM (Y102A). Codons are optimized for bacteria expression.

   Depositor

   Thomas Tuschl

   Article

   Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

   Insert

   Dnd1 (DND1 Human)
   

   Tags

   6xHIS and FLAG

   Expression

   Bacterial

   Mutation

   Y102A. Codon optimized for bacterial expression

   Available Since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET11d-FHis-DND1-Y102A

   Plasmid

   #70082

   Purpose

   Bacterial expression of FLAG-and 6xHis-tagged human DND1 protein with defective RRM (Y102A). Codons are optimized for bacteria expression.

   Depositor

   Thomas Tuschl

   Article

   Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

   Insert

   Dnd1 (DND1 Human)
   

   Tags

   6xHIS and FLAG

   Expression

   Bacterial

   Mutation

   Y102A. Codon optimized for bacterial expression

   Available Since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LeGO-iBSD-5LO-d4

   Plasmid

   #182743

   Purpose

   Lentiviral expression vector for codon-optimized 5-Lipoxygenase (isoform delta 4) in mamallian cells with blasticidin S resistance (bsd).

   Depositor

   Dieter Steinhilber

   Article

   Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d

   Insert

   5-Lipoxygenase isoform delta 4 (ALOX5 Human)
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Mutation

   codon optimized for expression in homo sapiens

   Promoter

   SFFV

   Available Since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LeGO-iBSD-5LO-d4

    Plasmid

    #182743

    Purpose

    Lentiviral expression vector for codon-optimized 5-Lipoxygenase (isoform delta 4) in mamallian cells with blasticidin S resistance (bsd).

    Depositor

    Dieter Steinhilber

    Article

    Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d

    Insert

    5-Lipoxygenase isoform delta 4 (ALOX5 Human)
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Mutation

    codon optimized for expression in homo sapiens

    Promoter

    SFFV

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LeGO-iBSD-5LO-p12

    Plasmid

    #182744

    Purpose

    Lentiviral expression vector for codon-optimized 5-Lipoxygenase (isoform p12) in mamallian cells with blasticidin S resistance (bsd).

    Depositor

    Dieter Steinhilber

    Article

    Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d

    Insert

    5-Lipoxygenase isoform p12 (ALOX5 Human)
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Mutation

    codon optimized for expression in homo sapiens

    Promoter

    SFFV

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LeGO-iBSD-5LO-p12

    Plasmid

    #182744

    Purpose

    Lentiviral expression vector for codon-optimized 5-Lipoxygenase (isoform p12) in mamallian cells with blasticidin S resistance (bsd).

    Depositor

    Dieter Steinhilber

    Article

    Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d

    Insert

    5-Lipoxygenase isoform p12 (ALOX5 Human)
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Mutation

    codon optimized for expression in homo sapiens

    Promoter

    SFFV

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LeGO-iBSD-5LO-E376Q

    Plasmid

    #182742

    Purpose

    Lentiviral expression vector for codon-optimized 5-Lipoxygenase (enzymatically inactive E376Q mutant) in mamallian cells with blasticidin S resistance (bsd).

    Depositor

    Dieter Steinhilber

    Article

    Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d

    Insert

    5-Lipoxygenase E376Q (ALOX5 Human)
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Mutation

    enzymatically inactive 5-LO E376Q mutant, codon o…

    Promoter

    SFFV

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LeGO-iBSD-5LO-E376Q

    Plasmid

    #182742

    Purpose

    Lentiviral expression vector for codon-optimized 5-Lipoxygenase (enzymatically inactive E376Q mutant) in mamallian cells with blasticidin S resistance (bsd).

    Depositor

    Dieter Steinhilber

    Article

    Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d

    Insert

    5-Lipoxygenase E376Q (ALOX5 Human)
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Mutation

    enzymatically inactive 5-LO E376Q mutant, codon o…

    Promoter

    SFFV

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LeGO-iBSD-5LO-d13

    Plasmid

    #182745

    Purpose

    Lentiviral expression vector for codon-optimized 5-Lipoxygenase (isoform delta 13) in mamallian cells with blasticidin S resistance (bsd).

    Depositor

    Dieter Steinhilber

    Article

    Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d

    Insert

    5-Lipoxygenase isoform delta 13 (ALOX5 Human)
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Mutation

    codon optimized for expression in homo sapiens

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LeGO-iBSD-5LO-d13

    Plasmid

    #182745

    Purpose

    Lentiviral expression vector for codon-optimized 5-Lipoxygenase (isoform delta 13) in mamallian cells with blasticidin S resistance (bsd).

    Depositor

    Dieter Steinhilber

    Article

    Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d

    Insert

    5-Lipoxygenase isoform delta 13 (ALOX5 Human)
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Mutation

    codon optimized for expression in homo sapiens

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTH848-gst-disec

    Plasmid

    #89589

    Purpose

    Plasmid for bacterial expression of His-tagged GST encoded by non-preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    glutathione-S transferase codon-dis-optimised for bacterial expression
    

    Tags

    8xhistidine

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTH848-gst-disec

    Plasmid

    #89589

    Purpose

    Plasmid for bacterial expression of His-tagged GST encoded by non-preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    glutathione-S transferase codon-dis-optimised for bacterial expression
    

    Tags

    8xhistidine

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pD10AH840AhCas9 (GB1041)

    Plasmid

    #68207

    Purpose

    Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed; human codon optimis…

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pD10AH840AhCas9 (GB1041)

    Plasmid

    #68207

    Purpose

    Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed; human codon optimis…

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge