We narrowed to 7,201 results for: TIL;

1. pAAV-mDLX-TVA-2A-N2cG

   Plasmid

   #172364

   Purpose

   Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from inhibitory neurons

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   TVA + B19-N2cG
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   mDLX

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. NAX_CAGGS_CAS9_m4_VPR_mcherry

   Plasmid

   #167886

   Purpose

   PiggyBac compatible plasmid expressing dCas9-VPR

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Mutation

   D10A, D839A, H840A, N863A

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

3. mcherry-Myo 9b R1695M

   Plasmid

   #134958

   Purpose

   Expression of rat Myo 9b (Myr 5) in mammalian cells.GAP minus mutant.

   Depositor

   Martin Bähler

   Article

   Hemkemeyer et al J Biol Chem. 2021 Jan-Jun;296:100136. do

   Insert

   Myo 9b (rat) with 3' UTR. GAP minus mutant R1695M (Myo9b Rat)
   

   Tags

   mcherry

   Expression

   Mammalian

   Promoter

   CMV IE

   Available Since

   Jan. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. CVS-N2c-nl.mCherry-FlpO

   Plasmid

   #172378

   Purpose

   Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   nuclear-localized mCherry + FlpO
   

   Use

   Neurotropic virus

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Syn-FLEX-ExSYTE

   Plasmid

   #216263

   Purpose

   Cre-dependent expressoin of ExSYTE (P2A) EGFP with 3'UTR of Rat Arc DTE under synapsin promoter

   Depositor

   Susumu Tomita

   Article

   Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

   Insert

   ExSYTE P2A EGFP - Rat DTE
   

   Use

   AAV

   Available Since

   May 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBR322-Pdp1_NTD-ZFD_R

   Plasmid

   #198277

   Purpose

   For loading R half of zinc finger deaminase targeting human TRAC locus (ZFD_TRAC-NC-Right-G1397-C_DddA); also contains PVC regulatory genes

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   Pdp1_NTD-ZFD_R
   

   Expression

   Bacterial

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTS1051-Tier1-PhCMV-4xCox8

   Plasmid

   #169490

   Purpose

   Tier-1 vector encoding PhCMV-driven four-fold repeat of a mitochondrial matrix localization signal derived from mitochondrial matrix protein Cox8 (PhCMV-4xCox8-pA)

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven mitochondrial localization signal from cytochrome c oxidase subunit 8
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21-pelB-LaG-41

   Plasmid

   #172757

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-41; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-41
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiEKAR2G2

   Plasmid

   #40178

   Depositor

   Olivier Pertz

   Article

   Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

   Insert

   Erk activity reporter
   

   Use

   Lentiviral

   Tags

   His-Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-EF1-IRES-NEO mutant ITR

    Plasmid

    #67905

    Purpose

    PiggyBac transposon encoding Neomycin resistance with inverted terminal repeat mutation

    Depositor

    Alex Kentsis

    Article

    Henssen et al Elife. 2015 Sep 25;4. pii: e10565. doi:

    Insert

    NeoR (neoR Synthetic)
    

    Expression

    Mammalian

    Available Since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHis-zfEpoa

    Plasmid

    #65611

    Purpose

    Express soluble His-tagged zebrafish erythropoietin in E. coli

    Depositor

    Petr Bartunek

    Article

    Stachura et al Blood. 2009 Jul 9;114(2):279-89. doi: 10

    Insert

    erythropoietin (epoa Zebrafish)
    

    Tags

    (His)6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21-pelB-LaG-26

    Plasmid

    #172751

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-26; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-26
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDR111_Bgal NLS

    Plasmid

    #188396

    Purpose

    Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.

    Depositor

    Christopher Contag

    Article

    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

    Insert

    lacZ
    

    Tags

    PhoD secretion peptide and SV40 NLS

    Expression

    Bacterial

    Promoter

    Phyper spank

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-C2-TetR-KRf-TetR

    Plasmid

    #216270

    Purpose

    Construct 3: TetR - Lipid binding domain KRφ - TetR

    Depositor

    Susumu Tomita

    Article

    Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

    Insert

    TetR - Lipid binding motif (KRf) - TetR
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    May 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. IA001: pMVP (L3-L2) 3x HA epitope tag+ polyA

    Plasmid

    #121750

    Purpose

    pMVP L3-L2 entry plasmid, contains 3x HA epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    3x HA epitope tag + polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUAST-GFP-RpL10Ab

    Plasmid

    #71729

    Purpose

    Expression of Drosophila melanogaster Rpl10Ab-GFP fusion

    Depositor

    Herman Dierick

    Article

    Thomas et al PLoS One. 2012;7(7):e40276. doi: 10.1371

    Insert

    RpL10Ab (RpL10Ab Fly)
    

    Tags

    EGFP

    Expression

    Insect

    Promoter

    hsp70

    Available Since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pOCC292

    Plasmid

    #118881

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mRuby3, and C-terminal HIS6 tag, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-mRuby3-3C-HIS6-stop-HindIII cassette
    

    Tags

    mRuby3, HIS6, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFA6a-kanMX6-PGAL1-GFP

    Plasmid

    #41614

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Available Since

    Dec. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFA6a-kanMX6-P3nmt1-3HA

    Plasmid

    #39283

    Depositor

    John Pringle, Jurg Bahler

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    nmt1 promoter (nmt1 Fission Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    3xHA, A. gossypii translation elongation factor 1…

    Mutation

    -1163 to +6 of nmt1 locus

    Available Since

    Aug. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. CVS-N2c-tdTomato-FlpO

    Plasmid

    #172375

    Purpose

    Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

    Depositor

    Yoav Ben-Simon, Peter Jonas

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    tdTomato + FlpO
    

    Use

    Neurotropic virus

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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