We narrowed to 9,603 results for: FIE

1. LentiCRISPRv2puro-Tbxas1

   Plasmid

   #170305

   Purpose

   A knock-out vector for the mouse Tbxas1

   Depositor

   Michiyuki Matsuda

   Article

   Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

   Insert

   A gRNA targeting the mouse Tbxas1 gene.
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. MMRN1-bio-His

   Plasmid

   #53409

   Purpose

   Expresses full-length Multimerin-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   MMRN1 (MMRN1 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. ELK4_pet28a

   Plasmid

   #131648

   Purpose

   Bacterial expression of ETS gene ELK4

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   ELK4 (ELK4 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. shPBRM1 #122-Tet-pLKO-puro

   Plasmid

   #107401

   Purpose

   Lentiviral expression of shRNA targeting PBRM

   Depositor

   William Kaelin

   Article

   Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

   Insert

   shRNA targeting PBRM1 (PBRM1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. ETV3L_pet28a

   Plasmid

   #131666

   Purpose

   Bacterial expression of ETS gene ETV3L

   Depositor

   Peter Hollenhorst

   Article

   Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

   Insert

   ETV3L (ETV3L Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEasyG1_nat

   Plasmid

   #184911

   Purpose

   Template to generate via PCR a single gRNA for expression in S. cerevisiae

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EF1a-DIO-mCherry-WPRE

   Plasmid

   #202620

   Purpose

   Double floxed red fluorescent protein mCherry in AAV production vector expressed under the mammalian promoter (EF1a)

   Depositor

   Francois St-Pierre

   Article

   Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

   Insert

   mCherry
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. cfmTurquoise-N

   Plasmid

   #139284

   Purpose

   Cys-free mTurquoise (improved ECFP) for extracellular FRET analysis. This variant contains the same set of mutations (C48S and C70M ) as cfSGFP2. Molecular brightness is similar to mTurquoise.

   Depositor

   Ikuo Wada

   Article

   Omae et al Transfusion. 2019 Jul;59(7):2429-2435. d

   Insert

   cfmTurquoise
   

   Use

   Tags

   Multi-cloning sites

   Expression

   Mammalian

   Mutation

   Cys48Ser and Cys70/Met

   Promoter

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. SPN-bio-His

   Plasmid

   #51829

   Purpose

   Expresses full-length Leukosialin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   SPN (SPN Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEXNKI-his-GST-3C-LIC

    Plasmid

    #108709

    Purpose

    Expression of a GST-6xHis-tagged target protein with 3C protease cleavage site.

    Depositor

    Titia Sixma

    Article

    Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

    Type

    Empty backbone

    Use

    Tags

    GST-6xHis-3C protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOPO685

    Plasmid

    #195300

    Purpose

    pBAD322-MycaIDCas-PaqCI, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is replaced with PaqCI entry site.

    Depositor

    Joseph Peters

    Article

    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

    Insert

    McCAST Cascade and native array with PaqCI entry site
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Arabinose inducible arapBAD

    Available since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. TMEM-bio-His

    Plasmid

    #51856

    Purpose

    Expresses full-length Transmembrane protein 109 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TMEM (TMEM109 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. A2M-bio-His

    Plasmid

    #53423

    Purpose

    Expresses full-length Alpha-2-macroglobulin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    A2M (A2M Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GFP-SP110C

    Plasmid

    #65393

    Purpose

    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

    Depositor

    Kyle Miller

    Article

    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

    Insert

    SP110C
    

    Use

    Tags

    EmGFP and V5

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

15. ELF5_pet28a

    Plasmid

    #131659

    Purpose

    Bacterial expression of ETS gene ELF5

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ELF5 (ELF5 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTG-mTDG-K341R

    Plasmid

    #52267

    Purpose

    Bacterial expression of mouse TDG-K341R with C-terminal GST-tag

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Insert

    TDG (Tdg Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    K341R, SUMO acceptor site mutation

    Promoter

    T7

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLXSN-H-Ras_V12

    Plasmid

    #39516

    Depositor

    Julian Downward

    Article

    Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

    Insert

    HRAS V12 (HRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    G12V

    Promoter

    Available since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

18. MSCV mouse Vav1 IRES GFP

    Plasmid

    #107091

    Purpose

    Mammalian expression retroviral vector

    Depositor

    Steven Clifford, Martine Roussel

    Article

    Lindsey et al Oncogene. 2015 Sep 3;34(36):4746-57. doi

    Insert

    Vav1 (Vav1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pETNKI-his-3C-LIC-kan

    Plasmid

    #108703

    Purpose

    Expression of an His-tagged target protein with 3C protease cleavage site.

    Depositor

    Titia Sixma

    Article

    Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

    Type

    Empty backbone

    Use

    Tags

    His-3C protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEasyG3_nat

    Plasmid

    #184918

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge