... in the membrane. An emerging interdisciplinary field of biology known as bioelectricity is revealing ...pulses. Other resources from the bioelectricity field In addition to sharing tools to study bioelectricity...bioelectricity, bioelectric researchers in many different fields came together to found the journal Bioelectricity...Bioelectricity in 2019 with a goal to broaden this field of research to the whole scientific community. Over the...bioelectricity is an extremely exciting emerging field, with many implications for regenerative medicine... the Allen Discovery Center. The future of this field has a high potential for the advancement of research...

...Multiple speakers from top minds in the stem cell field have described the Yamanaka paper as "life changing...keynote address, appeared almost incredulous that the field has exploded so quickly, citing the first clinical...as a prime example of the fast movement in this field.  As an Addgene scientist, I'm exposed to many scientific... I only get to scratch the surface of different fields. This meeting has given me an opportunity to dive...role in the scientific endeavor – particularly in fields like stem cell biology where questions of ethics...iPSC symposium, a panel of thought leaders in the field--including Shinya Yamanaka, George Q. Daley, Christine...thriving in a collaborative, rather than a combative, field. Of course they are competing with each other, but...

...transposon? The piggyBac™ transposon was originally identified in moths in 1983, but wasn’t successfully used...including making transgenic cell lines and genetically-modified cell lines. Furthermore, the use of piggyBac™ ...

...are resolvable compact identifiers, which are Compact Uniform Resource Identifiers (CURIEs) combined with...helpful, but it lacks context unless it links to a verified description of that reagent online. Furthermore...company and catalog number changes) the original identifier loses its meaning, making it difficult or impossible...long-term solution is the use of unique persistent identifiers (PIDs), a long-lasting way to identify and reference..., or physical reagents. What are persistent identifiers? Perhaps the most commonly known form of a unique...unique PID is the Digital Object Identifier (DOI). Nearly all the papers or preprints you read or produce...identify a plasmid available through Addgene and find verified information about each plasmid item. Given a specific...

...and must be amplified before use (Figure 8C), following the amplification protocol specified by the depositing... eliminates foreign DNA, which scientists have modified for a wide range of genome engineering techniques...nucleotide spacer that defines the genomic target to be modified. You can therefore change the genomic target of...is the most popular Cas endonuclease, with many modified versions engineered to increase specificity and...Cas enzyme, such as Cas9 or Cas12a. These are identified in their names — AcrII proteins inhibit Cas9 ...sequence. HDR efficiency is generally low (< 10% of modified alleles). Selecting a target cut site as close... another within a target locus of about 8 bp, specified by a gRNA. There are two main types of base edits...

...preparation. Figure 1: Silver staining of purified and non-purified AAV subjected to gel electrophoresis. ...adenoviral helper sequences. AAV preparations are purified by iodixanol gradient ultracentrifugation and ...and an internal control of known titer (protocol modified from Lock et al. (2014) (Link opens in a new window...within representative vector preparations was quantified with electron microscopy after negative staining...staining. Empty vector particles can be identified after negative staining and appear darker than full vector...droplet digital PCR (ddPCR) titration protocol (modified from Wang et al. (2018) (Link opens in a new window...

... Scorer 2.0 From the Church Lab : a tool that identifies putative target sites for S. pyogenes Cas9, S... Cas-Designer From the Kim Lab , Cas-OFFinder identifies gRNA target sequences from an input sequence .... CCTop - CRISPR/Cas9 target online predictor Identifies candidate sgRNA target sites by off-target quality...fission yeast. Target Finder (Feng Zhang lab) Identifies gRNA target sequences from an input sequence ...chicken, dog, mosquito, and stickleback. E-CRISP Identifies gRNA target sequences from an input sequence ... genomes From the Church Lab , a program that identifies gRNA target sequences from an input sequence,... Finder Tool from the O'Connor-Giles Lab that identifies gRNA target sequences from an input sequence ...

...Sanger Sequencing Purified recombinant antibody preps contain low levels of co-purified plasmid from transfection...production methods and quality control processes for purified recombinant antibodies. Antibody.... Antibodies are expressed for 7 days and then purified from the supernatant using Protein A or Protein...transfection. This residual plasmid is extracted and amplified with primers targeting the heavy chain and/or ...light chain variable regions. The PCR product is purified, sequenced via the Sanger method, and aligned ...

... typical plasmid and thus can be easily modified and amplified (through standard cloning methods). There...generate virus (left). B) Viral plasmids can be modified for research purposes such that, when delivered...delivered to a host, they give rise to viruses with modified viral genomes. Naturally occurring viruses harbor...generate virus. Furthermore, these plasmids can be modified to give rise to viral genomes of choice (Fig. ... maze. These engineered viral genomes are also modified (relative to natural viruses) for safety through...

...putative knockout allele must be experimentally verified. Edit Generate a specific user-defined sequence...embryos Cas9-gRNA ribonucleoprotein (RNP) complexes Purified Cas9 protein and in vitro transcribed gRNA are...sequences and putative target sites have been identified, it is time to choose which site is likely to...cell population. The region of interest is PCR amplified, PCR products are denatured-renatured, treated...restriction site. The region of interest is PCR amplified, digested with the appropriate restriction enzyme...insertions, the region of interest can be PCR amplified using primers that (A) flank the region of interest...

...tropism of this virus was modified so that it could only infect a genetically specified neuronal population....monosynpatic retrograde neuronal tracing, to be used with modified rabies virus. Viral...monosynaptic tracing. These AAV can be used with modified rabies virus to perform retrograde neuronal tracing...infected. Schematic depicting steps for using AAV and modified rabies virus (RABV) for monoclonal neuronal tracing...

...homology, these crRNAs guide a Cas nuclease to the specified exogenous genetic material, which must also contain...but it is not invincible. Bondy-Denomy et al. identified the first set of anti-CRISPR genes in bacteriophages...mutations. Researchers have expanded the CRISPR field beyond SpCas9, moving us closer to being able to...manner. Epigenetics : dCas9 fused to epigenetic modifiers can modulate transcriptions and create heritable... protocols , providing tips from experts in the field, and enabling access to multiple plasmids used for...

... tRNA/Synthetase Pairs Bacterial Strains Codon Modified Constructs The genetic code for all life is based...growing peptide chain. To expand the genetic code, modified tRNAs, codons, and tRNA synthetases are introduced...plasmid containing the gene of interest with the modified codon (typically the amber codon) that is recognized...below highlights bacteria strains that have been modified to enhance non-standard amino acid incorporation...below highlights plasmids that contain genes with modified codons for unnatural amino acid incorporation....

... the brain works. The neurodegenerative disease field will advance more quickly with the avid sharing ...methods, and results that are being adopted in the field. Read on for a brief introduction to the genetics...decision-making and language abilities. The disease can be classified as early-onset, where symptoms appear between...Includes antibodies, viral vectors, animal models, purified protein, and more. Plasmids provided by the foundation...

...Luciferase Tools in our Collection Modified/Novel Luciferases AkaLuc : A modified firefly luciferase engineered...control of a HSV TK promoter for normalization. Modified from psi-CHECK2 so both Renilla and Firefly luciferase... plasmid. Malcolm Moore 51819 Syn-ATP Firefly (modified) CMV Optical reporter of presynaptic ATP Timothy...

...expanding. Addgene works with leading scientists in the field to ensure our CRISPR collections and resources are...and more. CRISPR History : Learn how CRISPR was modified from a bacterial immune system to a revolutionary...transcription. Epigenetics dCas9 fused to an epigenetic modifier alters chromatin structure/gene expression. RNA...

...account verification. Once your account has been verified, you will be able to place your order on our website...portion of Addgene’s collection is available to qualified for-profit entities. Addgene reserves the right...right to determine if an entity is qualified to receive materials from our repository. All purchases are...

...specific DNA sequence ( Figure 1A ). Scientists have identified a large number of zinc fingers that recognize...with ZF arrays, if not slightly lower. Figure 2: Simplified representation of the Voytas/Bogdanove Golden...has considerable experience in the DNA targeting field. A third kit, a "TALe Toolbox", from Dr. Feng Zhang...

...proteins. These are antibody mimics instead of modified antibodies and are not closely related to antibodies... with: a positive control, a sample previously verified to contain your protein of interest (in ELISAs...released from the complex and can be sequenced and amplified for analysis. Overview of application Process ...assays. This method is suitable for DNA:histone modifier interactions, which have strong binding between...

...AAV2_586_PLGLAR Recombinant AAV2 rep/cap plasmid modified to include an 18 amino acid peptide at cap position...packaging vector carrying AAV2 rep gene and Cap8R modified AAV8 capsid coding sequence Lu 213974 AAV-DJ8R...packaging vector containing AAV2-rep gene and CapDJR modified AAV-DJ8 capsid coding sequence Lu AAV Plasmids...