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We narrowed to 667 results for: FIE

Showing: 1 - 20 of 667 results

  1. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    ... in the membrane. An emerging interdisciplinary field of biology known as bioelectricity is revealing ...pulses. Other resources from the bioelectricity field In addition to sharing tools to study bioelectricity...bioelectricity, bioelectric researchers in many different fields came together to found the journal Bioelectricity...Bioelectricity in 2019 with a goal to broaden this field of research to the whole scientific community. Over the...bioelectricity is an extremely exciting emerging field, with many implications for regenerative medicine... the Allen Discovery Center. The future of this field has a high potential for the advancement of research...

  2. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...Multiple speakers from top minds in the stem cell field have described the Yamanaka paper as "life changing...keynote address, appeared almost incredulous that the field has exploded so quickly, citing the first clinical...as a prime example of the fast movement in this field.  As an Addgene scientist, I'm exposed to many scientific... I only get to scratch the surface of different fields. This meeting has given me an opportunity to dive...role in the scientific endeavor – particularly in fields like stem cell biology where questions of ethics...iPSC symposium, a panel of thought leaders in the field--including Shinya Yamanaka, George Q. Daley, Christine...thriving in a collaborative, rather than a combative, field. Of course they are competing with each other, but...

  3. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...transposon? The piggyBac™ transposon was originally identified in moths in 1983, but wasn’t successfully used...including making transgenic cell lines and genetically-modified cell lines. Furthermore, the use of piggyBac™ ...

  4. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type
    Blog Post
    ...are resolvable compact identifiers, which are Compact Uniform Resource Identifiers (CURIEs) combined with...helpful, but it lacks context unless it links to a verified description of that reagent online. Furthermore...company and catalog number changes) the original identifier loses its meaning, making it difficult or impossible...long-term solution is the use of unique persistent identifiers (PIDs), a long-lasting way to identify and reference..., or physical reagents. What are persistent identifiers? Perhaps the most commonly known form of a unique...unique PID is the Digital Object Identifier (DOI). Nearly all the papers or preprints you read or produce...identify a plasmid available through Addgene and find verified information about each plasmid item. Given a specific...

  5. CRISPR Guide

    Type
    Collection
    ...and must be amplified before use (Figure 8C), following the amplification protocol specified by the depositing... eliminates foreign DNA, which scientists have modified for a wide range of genome engineering techniques...nucleotide spacer that defines the genomic target to be modified. You can therefore change the genomic target of...is the most popular Cas endonuclease, with many modified versions engineered to increase specificity and...Cas enzyme, such as Cas9 or Cas12a. These are identified in their names — AcrII proteins inhibit Cas9 ...sequence. HDR efficiency is generally low (< 10% of modified alleles). Selecting a target cut site as close... another within a target locus of about 8 bp, specified by a gRNA. There are two main types of base edits...

  6. Viral Production

    Type
    Collection
    ...preparation. Figure 1: Silver staining of purified and non-purified AAV subjected to gel electrophoresis. ...adenoviral helper sequences. AAV preparations are purified by iodixanol gradient ultracentrifugation and ...and an internal control of known titer (protocol modified from Lock et al. (2014) (Link opens in a new window...within representative vector preparations was quantified with electron microscopy after negative staining...staining. Empty vector particles can be identified after negative staining and appear darker than full vector...droplet digital PCR (ddPCR) titration protocol (modified from Wang et al. (2018) (Link opens in a new window...

  7. Antibody Production

    Type
    Collection
    ...Sanger Sequencing Purified recombinant antibody preps contain low levels of co-purified plasmid from transfection...production methods and quality control processes for purified recombinant antibodies. Antibody.... Antibodies are expressed for 7 days and then purified from the supernatant using Protein A or Protein...transfection. This residual plasmid is extracted and amplified with primers targeting the heavy chain and/or ...light chain variable regions. The PCR product is purified, sequenced via the Sanger method, and aligned ...

  8. Viral Vectors

    Type
    Collection
    ... typical plasmid and thus can be easily modified and amplified (through standard cloning methods). There...generate virus (left). B) Viral plasmids can be modified for research purposes such that, when delivered...delivered to a host, they give rise to viruses with modified viral genomes. Naturally occurring viruses harbor...generate virus. Furthermore, these plasmids can be modified to give rise to viral genomes of choice (Fig. ... maze. These engineered viral genomes are also modified (relative to natural viruses) for safety through...

  9. CRISPR References and Information

    Type
    Collection
    ... 2.0 (Link opens in a new window) A tool that identifies putative target sites for S. pyogenes Cas9, S...Institute. Cas-OFFinder (Link opens in a new window) Identifies gRNA target sequences from an input sequence ...online predictor (Link opens in a new window) Identifies candidate sgRNA target sites by off-target quality...Lab . CRISPRdirect (Link opens in a new window) Identifies candidate gRNA target sequences in an input sequence...Kim Labs. E-CRISPR (Link opens in a new window) Identifies gRNA target sequences from an input sequence ...genomes (Link opens in a new window) A program that identifies gRNA target sequences from an input sequence,...

  10. Plan Your Experiment

    Type
    Collection
    ...putative knockout allele must be experimentally verified. Edit Generate a specific user-defined sequence...embryos Cas9-gRNA ribonucleoprotein (RNP) complexes Purified Cas9 protein and in vitro transcribed gRNA are...sequences and putative target sites have been identified, it is time to choose which site is likely to...cell population. The region of interest is PCR amplified, PCR products are denatured-renatured, treated...restriction site. The region of interest is PCR amplified, digested with the appropriate restriction enzyme...insertions, the region of interest can be PCR amplified using primers that (A) flank the region of interest...

  11. AAV for Neuronal Tracing

    Type
    Collection
    ...tropism of this virus was modified so that it could only infect a genetically specified neuronal population....monosynpatic retrograde neuronal tracing, to be used with modified rabies virus. Viral...monosynaptic tracing. These AAV can be used with modified rabies virus to perform retrograde neuronal tracing...infected. Schematic depicting steps for using AAV and modified rabies virus (RABV) for monoclonal neuronal tracing...

  12. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...homology, these crRNAs guide a Cas nuclease to the specified exogenous genetic material, which must also contain...but it is not invincible. Bondy-Denomy et al. identified the first set of anti-CRISPR genes in bacteriophages...mutations. Researchers have expanded the CRISPR field beyond SpCas9, moving us closer to being able to...manner. Epigenetics : dCas9 fused to epigenetic modifiers can modulate transcriptions and create heritable... protocols , providing tips from experts in the field, and enabling access to multiple plasmids used for...

  13. Genetic Code Expansion

    Type
    Collection
    ... tRNA/Synthetase Pairs Bacterial Strains Codon Modified Constructs The genetic code for all life is based...growing peptide chain. To expand the genetic code, modified tRNAs, codons, and tRNA synthetases are introduced...plasmid containing the gene of interest with the modified codon (typically the amber codon) that is recognized...below highlights bacteria strains that have been modified to enhance non-standard amino acid incorporation...below highlights plasmids that contain genes with modified codons for unnatural amino acid incorporation....

  14. Neurodegeneration Research Collection

    Type
    Collection
    ... the brain works. The neurodegenerative disease field will advance more quickly with the avid sharing ...methods, and results that are being adopted in the field. Read on for a brief introduction to the genetics...decision-making and language abilities. The disease can be classified as early-onset, where symptoms appear between...Includes antibodies, viral vectors, animal models, purified protein, and more. Plasmids provided by the foundation...

  15. Luciferase Plasmid Collection

    Type
    Collection
    ...Luciferase Tools in our Collection Modified/Novel Luciferases AkaLuc : A modified firefly luciferase engineered...control of a HSV TK promoter for normalization. Modified from psi-CHECK2 so both Renilla and Firefly luciferase... plasmid. Malcolm Moore 51819 Syn-ATP Firefly (modified) CMV Optical reporter of presynaptic ATP Timothy...

  16. CRISPR Plasmids and Resources

    Type
    Collection
    ...expanding. Addgene works with leading scientists in the field to ensure our CRISPR collections and resources are...and more. CRISPR History : Learn how CRISPR was modified from a bacterial immune system to a revolutionary...transcription. Epigenetics dCas9 fused to an epigenetic modifier alters chromatin structure/gene expression. RNA...

  17. Distribution to Industry

    Type
    Collection
    ...account verification. Once your account has been verified, you will be able to place your order on our website...portion of Addgene’s collection is available to qualified for-profit entities. Addgene reserves the right...right to determine if an entity is qualified to receive materials from our repository. All purchases are...

  18. TALEN Guide

    Type
    Collection
    ...specific DNA sequence ( Figure 1A ). Scientists have identified a large number of zinc fingers that recognize...with ZF arrays, if not slightly lower. Figure 2: Simplified representation of the Voytas/Bogdanove Golden...has considerable experience in the DNA targeting field. A third kit, a "TALe Toolbox", from Dr. Feng Zhang...

  19. Antibody Guide

    Type
    Collection
    ...proteins. These are antibody mimics instead of modified antibodies and are not closely related to antibodies... with: a positive control, a sample previously verified to contain your protein of interest (in ELISAs...released from the complex and can be sequenced and amplified for analysis. Overview of application Process ...assays. This method is suitable for DNA:histone modifier interactions, which have strong binding between...

  20. Antibodies

    Type
    Collection
    ...collection. These affinity reagents are expressed and purified from plasmids in our repository....We work with trusted partners and experts in the field to develop new antibodies and provide experimental...
Showing: 1 - 20 of 667 results