We narrowed to 56 results for: FIE

...and must be amplified before use (Figure 8C), following the amplification protocol specified by the depositing... eliminates foreign DNA, which scientists have modified for a wide range of genome engineering techniques...nucleotide spacer that defines the genomic target to be modified. You can therefore change the genomic target of...is the most popular Cas endonuclease, with many modified versions engineered to increase specificity and...Cas enzyme, such as Cas9 or Cas12a. These are identified in their names — AcrII proteins inhibit Cas9 ...sequence. HDR efficiency is generally low (< 10% of modified alleles). Selecting a target cut site as close... another within a target locus of about 8 bp, specified by a gRNA. There are two main types of base edits...

...preparation. Figure 1: Silver staining of purified and non-purified AAV subjected to gel electrophoresis. ...adenoviral helper sequences. AAV preparations are purified by iodixanol gradient ultracentrifugation and ...and an internal control of known titer (protocol modified from Lock et al. (2014) (Link opens in a new window...within representative vector preparations was quantified with electron microscopy after negative staining...staining. Empty vector particles can be identified after negative staining and appear darker than full vector...droplet digital PCR (ddPCR) titration protocol (modified from Wang et al. (2018) (Link opens in a new window...

...Sanger Sequencing Purified recombinant antibody preps contain low levels of co-purified plasmid from transfection...production methods and quality control processes for purified recombinant antibodies. Antibodies.... Antibodies are expressed for 7 days and then purified from the supernatant using Protein A or Protein...transfection. This residual plasmid is extracted and amplified with primers targeting the heavy chain and/or ...light chain variable regions. The PCR product is purified, sequenced via the Sanger method, and aligned ...

... 2.0 (Link opens in a new window) A tool that identifies putative target sites for S. pyogenes Cas9, S...Institute. Cas-OFFinder (Link opens in a new window) Identifies gRNA target sequences from an input sequence ...online predictor (Link opens in a new window) Identifies candidate sgRNA target sites by off-target quality...Lab . CRISPRdirect (Link opens in a new window) Identifies candidate gRNA target sequences in an input sequence...Kim Labs. E-CRISPR (Link opens in a new window) Identifies gRNA target sequences from an input sequence ...genomes (Link opens in a new window) A program that identifies gRNA target sequences from an input sequence,...

...tropism of this virus was modified so that it could only infect a genetically specified neuronal population....monosynpatic retrograde neuronal tracing, to be used with modified rabies virus. Viral...monosynaptic tracing. These AAV can be used with modified rabies virus to perform retrograde neuronal tracing...infected. Schematic depicting steps for using AAV and modified rabies virus (RABV) for monoclonal neuronal tracing...

...homology, these crRNAs guide a Cas nuclease to the specified exogenous genetic material, which must also contain...but it is not invincible. Bondy-Denomy et al. identified the first set of anti-CRISPR genes in bacteriophages...mutations. Researchers have expanded the CRISPR field beyond SpCas9, moving us closer to being able to...manner. Epigenetics : dCas9 fused to epigenetic modifiers can modulate transcriptions and create heritable... protocols , providing tips from experts in the field, and enabling access to multiple plasmids used for...

... the brain works. The neurodegenerative disease field will advance more quickly with the avid sharing ...methods, and results that are being adopted in the field. Read on for a brief introduction to the genetics...decision-making, and language abilities. The disease can be classified as early-onset, where symptoms appear between...Includes antibodies, viral vectors, animal models, purified protein, and more. Plasmids provided by the foundation...

...expanding. Addgene works with leading scientists in the field to ensure our CRISPR collections and resources are...and more. CRISPR History : Learn how CRISPR was modified from a bacterial immune system to a revolutionary...transcription. Epigenetics dCas9 fused to an epigenetic modifier alters chromatin structure/gene expression. RNA...

...putative knockout allele must be experimentally verified Cut Nick Homology Directed Repair (HDR) Generate..., you can also directly deliver mature mRNA or purified protein into cells. These methods are more transient... This method is a common choice for generating modified mouse lines. Protein delivery Lastly, you can ...mRNA to protein. Instead, you directly combine purified Cas protein and in vitro transcribed gRNA to form...

...account verification. Once your account has been verified, you will be able to place your order on our website...portion of Addgene's collection is available to qualified for-profit entities. Addgene reserves the right...right to determine if an entity is qualified to receive materials from our repository. All purchases are...

...specific DNA sequence ( Figure 1A ). Scientists have identified a large number of zinc fingers that recognize...with ZF arrays, if not slightly lower. Figure 2: Simplified representation of the Voytas/Bogdanove Golden...has considerable experience in the DNA targeting field. A third kit, a "TALe Toolbox", from Dr. Feng Zhang...

...proteins. These are antibody mimics instead of modified antibodies and are not closely related to antibodies... with: a positive control, a sample previously verified to contain your protein of interest (in ELISAs...released from the complex and can be sequenced and amplified for analysis. Overview of application Process ...assays. This method is suitable for DNA:histone modifier interactions, which have strong binding between...

...control of a HSV TK promoter for normalization. Modified from psi-CHECK2 so both Renilla and Firefly luciferase... plasmid. Malcolm Moore 51819 Syn-ATP Firefly (modified) CMV Optical reporter of presynaptic ATP Timothy...Insert PI Return to Top Other Highlighted Tools Modified/Novel Luciferases Engineered variants of luciferases...

...collection. These affinity reagents are expressed and purified from plasmids in our repository....We work with trusted partners and experts in the field to develop new antibodies and provide experimental...

...catalytically dead Cas9 (dCas9) to epigenetic modifiers. Common modifiers include: P300 — activation through increased...

... and ready to ship. All viral vector preps are purified by density centrifugation. Addgene’s comprehensive...AAV preps are distributed as 100 µL solutions of purified viral particles that are suitable for in vivo ...

... the BRAIN Initiative below. Support has been identified by depositors and/or when BRAIN Initiative grants...high-quality and high-titer 100 µL solutions of purified viral particles that are suitable for in vivo ...our repository. AAV are distributed as 100 µL of purified viral particles in solution that are suitable ...

...plasmids are created by your colleagues, sequence-verified, and widely cited in publications. All pricing...virus-associated DNA, which is a sample of the purified DNA from the original plasmid that was used to...

...transporters in the human genome with approximately 450 identified members. Many SLCs have mutations associated ...expects to accelerate the pace of research in the field of SLCs to the global benefit of basic academic ...

...If any discrepancies are identified during this analysis, you will be notified by email. If Addgene determines...