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We narrowed to 13 results for: FIE

Showing: 1 - 13 of 13 results

  1. CRISPR Guide

    Type
    Guide
    ...and must be amplified before use (Figure 8C), following the amplification protocol specified by the depositing... eliminates foreign DNA, which scientists have modified for a wide range of genome engineering techniques...nucleotide spacer that defines the genomic target to be modified. You can therefore change the genomic target of...is the most popular Cas endonuclease, with many modified versions engineered to increase specificity and...Cas enzyme, such as Cas9 or Cas12a. These are identified in their names — AcrII proteins inhibit Cas9 ...sequence. HDR efficiency is generally low (< 10% of modified alleles). Selecting a target cut site as close... another within a target locus of about 8 bp, specified by a gRNA. There are two main types of base edits...

  2. Molecular Biology Reference

    Type
    Guide
    ...sequence. This obstacle has been overcome by the field of recombinant DNA technology, which enables the... to a plasmid. These tools are essential to the field of recombinant DNA, in which many identical DNA ...Cloning Plasmids that are used most commonly in the field of recombinant DNA technology have been optimized... for the ability of the plasmid to be copied (amplified) by bacteria, which is an important characteristic...Stable - Plasmids are stable long-term either as purified DNA or within bacterial cells that have been preserved... promoter. Viral Plasmids - These plasmids are modified viral genomes that are used to efficiently deliver...generally propagated, selected for, and the integrity verified prior to use in an experiment. E. coli strains...

  3. Adenovirus Guide

    Type
    Guide
    ...60 serotypes have been identified within human adenoviruses, which are classified into seven species (A...Adenoviral Vectors Wild-type adenoviruses have been modified by researchers to create recombinant adenoviral...of interest is cloned into the shuttle plasmid, verified, and linearized with the restriction enzyme PmeI...plasmids. Recombinant adenoviral plasmids are then verified for size and proper restriction digest patterns...patterns of recombination have not occurred. Once verified, the recombinant plasmid is linearized with PacI...expression, rAdV vectors are used in many research fields, especially when needing transgenes to express ...., Kinzler, K. W., & Vogelstein, B. (1998). A simplified system for generating recombinant adenoviruses...

  4. Plan Your Experiment

    Type
    Guide
    ...putative knockout allele must be experimentally verified. Edit Generate a specific user-defined sequence...embryos Cas9-gRNA ribonucleoprotein (RNP) complexes Purified Cas9 protein and in vitro transcribed gRNA are...sequences and putative target sites have been identified, it is time to choose which site is likely to...cell population. The region of interest is PCR amplified, PCR products are denatured-renatured, treated...restriction site. The region of interest is PCR amplified, digested with the appropriate restriction enzyme...insertions, the region of interest can be PCR amplified using primers that (A) flank the region of interest...

  5. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...biomedical fields. Gene Therapy One of the major applications of rAAV vectors is in the field of gene therapy...Recombinant AAV Vectors Wild-type AAVs have been modified by researchers to create recombinant adeno-associated...produced in HEK293T cells are then extracted and purified for their use as viral vectors to deliver genes...and primate serotypes of AAV have thus far been identified, and it is likely that this number will continue...Recombinant adeno-associated viral (rAAV) vector Modified form of AAV used to deliver genetic material into...

  6. Optogenetics Guide

    Type
    Guide
    ...Opsins Optical Switches References Introduction The field of optogenetics integrates optics and genetic engineering... activated. Optogenetics tools can be broadly classified based on their functions into two groups: Actuators...ions into or out of the cell. Scientists have identified a variety of naturally occurring microbial opsins...variants that inhibit neurons have been created and identified in other species - by acting as light-gated chloride...

  7. Antibody Guide

    Type
    Guide
    ...proteins. These are antibody mimics instead of modified antibodies and are not closely related to antibodies... with: a positive control, a sample previously verified to contain your protein of interest (in ELISAs...released from the complex and can be sequenced and amplified for analysis. Overview of application Process ...assays. This method is suitable for DNA:histone modifier interactions, which have strong binding between...

  8. Cloning

    Type
    Guide
    ...). To do this your DNA fragment must first be amplified with specific Gateway attB1 and attB2 sites attached...endonucleases. These endonucleases cut dsDNA at a specified distance away from the recognition sequence. Cutting...cloning, but instead of an in vitro reaction with purified enzymes, it takes advantage of the powerful recombination...

  9. Chemogenetics Guide

    Type
    Guide
    ...different types of DREADDs that can be broadly classified by the signaling protein that the receptors couple...inhibit neuronal signaling. PSAM-based LGICs can be classified by the ion pore domain they are coupled to: PSAM-Gly...chemogenetic control in a single molecule driven by FRET-modified bioluminescence. Neurophotonics. 11:021005. PMID...

  10. Guide to Using Pooled Libraries

    Type
    Guide
    ...the library information to see if it should be amplified before you conduct your screen. If you need to...surviving cells are collected, their plasmids are PCR-amplified and sequenced using NGS to identify their cDNA...

  11. Lentiviral Vector Guide

    Type
    Guide
    ...2018). Differential viral accessibility (DIVA) identifies alterations in chromatin architecture through... can infect and replicate in. Viral vector The modified form of a wild-type virus used to deliver genetic...

  12. Science Guides

    Type
    Guide
    ... adaptive immune system in bacteria, have been modified for genome engineering. Find essential background...

  13. Gamma-Retroviral Vector Guide

    Type
    Guide
    ... can infect and replicate in. Viral vector The modified form of a wild-type virus used to deliver genetic...
Showing: 1 - 13 of 13 results