We narrowed to 5,605 results for: pcdna3 1

1. pcDNA3-LplA(W37V) Coumarin PRIME ligase

   Plasmid

   #25845

   Depositor

   Alice Ting

   Article

   Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

   Insert

   LplA-W37V
   

   Use

   Tags

   Flag and His

   Expression

   Mammalian

   Mutation

   W37V

   Promoter

   Available since

   July 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLAG-MBP1-E322TAG-E309C-W340A-CAAX.pcDNA3-k

   Plasmid

   #127409

   Purpose

   Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

   Depositor

   Sharona Gordon

   Article

   Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

   Insert

   Maltose Binding Protein
   

   Use

   Tags

   CAAX and FLAG

   Expression

   Mammalian

   Mutation

   Amino acid mutations: E322 to Amber codon, E309C …

   Promoter

   CMV

   Available since

   July 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-MBP1-E322TAG-K305H-E309H-W340A.pcDNA3-k

   Plasmid

   #127406

   Purpose

   Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

   Depositor

   Sharona Gordon

   Article

   Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

   Insert

   Maltose Binding Protein
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Amino acid mutations: E322 to Amber codon, K305H,…

   Promoter

   CMV

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. FLAG-MBP1-K295TAG-W340A-S233H-T237C.pcDNA3-k

   Plasmid

   #127402

   Purpose

   Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site

   Depositor

   Sharona Gordon

   Article

   Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

   Insert

   Maltose Binding Protein
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Amino acid mutations: K295 to Amber codon, W340A,…

   Promoter

   CMV

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. FLAG-MBP1-K295TAG-W340A-S233H-T237H.pcDNA3-k

   Plasmid

   #127400

   Purpose

   Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site

   Depositor

   Sharona Gordon

   Article

   Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

   Insert

   Maltose Binding Protein
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Amino acid mutations: K295 to Amber codon, W340A,…

   Promoter

   CMV

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-MAVS-M1A,M142A-invitro(KaganE14)

   Plasmid

   #52018

   Purpose

   Used for in vitro expression of the human MAVS CDS containing the point mutations M1A,M142A

   Depositor

   Jonathan Kagan

   Article

   Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

   Insert

   MAVS-M1A,M142A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Met 1 to Ala and Met 142 to Ala

   Promoter

   Available since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3-N-mSOG-FRB-IRES-mCherry

   Plasmid

   #136602

   Purpose

   Encodes FRB tagged with the N-terminal fragment of split-miniSOG (mSOG1-94).

   Depositor

   John Ngo

   Article

   Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

   Insert

   N-mSOG-FRB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3.1-Flag-H2A K118-119R

   Plasmid

   #63564

   Purpose

   Expression of human H2A with K118R and K119R tagged with Flag

   Depositor

   Titia Sixma

   Article

   Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

   Insert

   H2A (H2AC20 Human)
   

   Use

   Tags

   Flag (MDYKDDDDKVPRIQ)

   Expression

   Mammalian

   Mutation

   Lysine 118 and Lysine 119 are now Arginine

   Promoter

   CMV

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pCDNA3.1-Flag-H2A K13-15R

   Plasmid

   #63563

   Purpose

   Expression of human H2A with K13R and K15R tagged with Flag

   Depositor

   Titia Sixma

   Article

   Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

   Insert

   H2A (H2AC20 Human)
   

   Use

   Tags

   Flag (MDYKDDDDKVPRIQ)

   Expression

   Mammalian

   Mutation

   Lysine 13 and Lysine 15 are now Arginine

   Promoter

   CMV

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3-3myc-6His-EZH2 21D

    Plasmid

    #42664

    Depositor

    Mien-Chie Hung

    Article

    Cha et al Science. 2005 Oct 14;310(5746):306-10.

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Tags

    3Myc and 6His

    Expression

    Mammalian

    Mutation

    S21D

    Promoter

    Available since

    July 17, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3-3myc-6His-EZH2 21A

    Plasmid

    #42663

    Depositor

    Mien-Chie Hung

    Article

    Cha et al Science. 2005 Oct 14;310(5746):306-10.

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Tags

    3Myc and 6His

    Expression

    Mammalian

    Mutation

    S21A

    Promoter

    Available since

    July 17, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.1-CMV-Puro-P2A-NES/PKI-mNeonGreen2x-NLS/FUS

    Plasmid

    #228400

    Purpose

    Encodes NES/PKI-mNeonGreen2x-NLS/FUS which is a tandem of mNeongreen with an N-terminal PKI NES and a C-terminal FUS NLS that shuttles between the nucleus and the cytoplasm

    Depositor

    Dirk Daelemans

    Article

    Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

    Insert

    NES/PKI-mNeonGreen2x-NLS/FUS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-HNF1B-WT-Flag-UTR

    Plasmid

    #183239

    Purpose

    Expression of FLAG tagged HNF1B

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Insert

    HNF1 homeobox B (HNF1B Human)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)-DnaJB1-PKAcat-mCherry

    Plasmid

    #181850

    Purpose

    mCherry-tagged DnaJB1-PKA catalytic subunit fusion; for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-Lyso-INPP4B-mCherry

    Plasmid

    #184055

    Purpose

    Lysosome targeted inositol polyphosphate-4-phosphatase type II B; tagged with mCherry.

    Depositor

    Jin Zhang

    Article

    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

    Insert

    LGP120 Lyso-INPP4B-mCherry (LAMP1, INPP4B Human)
    

    Use

    Tags

    Lysosome-associated membrane protein 1 (LAMP1). a…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. XE129 beta2AR Fz1-pcDNA3.1

    Plasmid

    #16795

    Depositor

    Randall Moon

    Article

    Liu et al Proc Natl Acad Sci U S A. 1999 Dec 7;96(

    Insert

    beta 2 AR Fz1 (Fzd1, Adrb2 Rat)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    Chimera of beta 2 adrenergic receptor (extracellu…

    Promoter

    Available since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3.1-Myc-BirA*-CAMK2D

    Plasmid

    #221686

    Purpose

    Mammalian expression of Myc-tagged BirA*-CAMK2D fusion protein for BioID

    Depositor

    Derrick Sek Tong Ong

    Article

    Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

    Insert

    CAMK2D (CAMK2D Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-HNF1B-FUS-IDR

    Plasmid

    #183240

    Purpose

    Expression of HNF1B fused to a intrinsically disordered regions of the FUS protein

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Insert

    HNF1 homeobox B (HNF1B Human)
    

    Use

    Tags

    Fusion to an IDR from the FUS protein and FLAG ta…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-Lyso-mCherry-Rheb(C181S)

    Plasmid

    #192438

    Purpose

    Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.

    Depositor

    Jin Zhang

    Article

    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

    Insert

    Lyso-mCherry-Rheb(C181S) (RHEB Human)
    

    Use

    Tags

    Full-length LAMP1 and mCherry

    Expression

    Mammalian

    Mutation

    Rheb Cys 181 mutated to Ser; abolishes C-terminal…

    Promoter

    CMV

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-PB1-3x-FLAG-B/BRISBANE/60/2008

    Plasmid

    #187918

    Purpose

    Expresses C terminal 3xFLAG tagged PB1 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

    Depositor

    Arindam Mondal

    Article

    Kedia et al Front Microbiol. 2022 May 25;13:868367.

    Insert

    polymerase basic protein 1
    

    Use

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    CMV Promoter

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge