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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 23,437 results for: CRISPR

Showing: 2741 - 2760 of 23437 results

  1. pHsp70-Cas9

    Plasmid
    #45945
    Purpose
    The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.
    Depositor
    Kate O'Connor-Giles, Jill Wildonger, Melissa Harrison
    Article
    Gratz et al Genetics. 2013 May 24.
    Insert
    Codon optimized Cas9
    Use
    CRISPR
    Tags
    3x FLAG
    Expression
    Mutation
    Silent change from C to A at bp 834 of insert
    Promoter
    Available Since
    July 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    45945-plasmid-map-sequence-id-116251

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  2. pSBY3_ligD-HygR

    Plasmid
    #104625
    Purpose
    CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD and hygromysin resistance gene.
    Depositor
    Sheng Yang
    Article
    Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d
    Insert
    FnCpf1
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    FnCpf1 codon-optimized C.glutamicum
    Promoter
    hsp60
    Available Since
    June 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    104625-plasmid-map-sequence-id-201573

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  7. pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

    Plasmid
    #41863
    Purpose
    This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.
    Depositor
    Feng Zhang
    Article
    Cong et al Science. 2013 Jan 3.
    Insert
    U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Feb. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    41863-plasmid-map-sequence-id-57052

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  12. pJL31

    Plasmid
    #227428
    Purpose
    Kanamycin resistant L5-integrative vector for CRISPR interference in M.smegmatis with constitutive expression of mWasabi under the control of the psmyc promotor (psmyc::mWasabi)
    Depositor
    Pierre Santucci
    Article
    Laudouze et al FEBS Lett. 2025 Feb;599(4):488-501. doi:
    Insert
    mWasabi
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    227428-plasmid-map-sequence-id-455967

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  13. pJL32

    Plasmid
    #227429
    Purpose
    Kanamycin resistant L5-integrative vector for CRISPR interference in M.smegmatis with the constitutive expression of tdTomato under the control of the psmyc promotor (psmyc::tdTomato)
    Depositor
    Pierre Santucci
    Article
    Laudouze et al FEBS Lett. 2025 Feb;599(4):488-501. doi:
    Insert
    tdTomato
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    227429-plasmid-map-sequence-id-455968

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  14. 3xFLAG-dCas9/pMXs-puro

    Plasmid
    #51240
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available Since
    Aug. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    51240-plasmid-map-sequence-id-410075

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Showing: 2741 - 2760 of 23437 results