We narrowed to 5,570 results for: donor

1. HR180-LGR5-CreER

   Plasmid

   #129093

   Purpose

   KI CreER with a puromycin/mRuby selection cassette into human LGR5 locus

   Depositor

   Toshiro Sato

   Article

   Shimokawa et al Nature. 2017 May 11;545(7653):187-192. d

   Insert

   CreERT2 and LGR5 homology arms (LGR5 Human)
   

   Use

   Homologous recombination donor plasmid

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAF0692 Ef1a-Dn29_attD-Puro-p2a-mCherry

   Plasmid

   #193465

   Purpose

   Dn29 Donor with promoter for mcherry and puro expression

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Dn29_attP
   

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pORANGE GFP-Actb KI #2

   Plasmid

   #139666

   Purpose

   Endogenous tagging of β-actin: N-terminal (amino acid position: before startcodon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pORANGE unc13a GFP KI

   Plasmid

   #131498

   Purpose

   Endogenous tagging of Munc13-1: C-terminal (amino acid position: A1762)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Unc13a Rat)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. FASTHDR-Cterm-mRuby3-Zeo

   Plasmid

   #167206

   Purpose

   Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mRuby3 and selection with Zeocin

   Depositor

   Oscar Perez

   Article

   Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   mRuby3

   Expression

   Mammalian

   Available Since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMIA10.53-Clover

   Plasmid

   #214450

   Purpose

   BxbI landing-pad mClover-IRES-puro donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates constitutively expressing cell line.

   Depositor

   Roger Foo

   Article

   Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755

   Insert

   mClover
   

   Expression

   Mammalian

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-eGFP-mPlum-HA

   Plasmid

   #200009

   Purpose

   Donor vector with double fluorescence system for targeted integration using CRISPR/Cas9. Expresses 5´HA--eGFP&PuroR--3´HA--mPlum. Distinguish targeted from random integration by fluorescence.

   Depositor

   Thomas Noll

   Article

   Hertel et al Front Bioeng Biotechnol. 2022 Oct 13;10:

   Insert

   eGFP, mPlum
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV ORANGE Dlg4-HaloTag KI

   Plasmid

   #139656

   Purpose

   Endogenous tagging of PSD95: C-terminal (amino acid position: R721)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and HaloTag donor
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDB2-hCRY2-Halo-CD4-bla

   Plasmid

   #189986

   Purpose

   Donor Vector to integrate HaloTag C-terminal into the human CRY2 Locus to express it as a fusion protein

   Depositor

   Achim Kramer

   Article

   Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

   Insert

   HaloTag
   

   Use

   CRISPR

   Tags

   3xFlag

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBR_attB(bxb)_ccdB_lox

    Plasmid

    #183763

    Purpose

    Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    ccdB
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDONR221-hSOX10

    Plasmid

    #24749

    Depositor

    William Pavan

    Article

    Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

    Insert

    (sex determining region Y)-box 10 (SOX10 Human)
    

    Use

    Gateway donor vector

    Mutation

    wildtype sequence

    Available Since

    May 18, 2010

    Availability

    Academic Institutions and Nonprofits only

12. FASTHDR-Cterm-mTagBFP2-Blasticidin

    Plasmid

    #167207

    Purpose

    Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mTagBFP2 and selection with Blasticidin

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    mTagBFP2

    Expression

    Mammalian

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SOX9-T2A-H2B-EGFP repair template

    Plasmid

    #167972

    Purpose

    SOX9 reporter repair template

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    SRY-Box Transcription Factor 9 (SOX9 Human)
    

    Use

    CRISPR; Donor template

    Tags

    H2B-EGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pORANGE Bsn-GFP KI

    Plasmid

    #139665

    Purpose

    Endogenous tagging of Bassoon: C-terminal (amino acid position: F3938)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PB-LacI-eGFP

    Plasmid

    #213021

    Purpose

    piggyBac donor vector containing LacI-eGFP for random integration by PBase

    Depositor

    Luca Giorgetti

    Article

    Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

    Insert

    LacI**-eGFP
    

    Use

    Mouse Targeting; Piggybase integration (transposo…

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PUC19_C_tag

    Plasmid

    #186659

    Purpose

    Empty donor plasmid for C-terminal tag of 3xFlag-3xHA.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    C-terminal 3xFlag-3xHA Tag
    

    Tags

    3xFlag-3xHA

    Expression

    Insect

    Available Since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Bxb1_AJMA

    Plasmid

    #183760

    Purpose

    Bxb1-specific donor plasmid for integrating transcription units for expressing ASAP2f (2 copies), jRCaMP1b and membrane-localised miRFP703

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Inserts

    ASAP2f
    jRCaMP1b
    miRFP703
    ASAP2f
    

    Tags

    Lck and NES

    Expression

    Mammalian

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. phROSA26-Tet-HA-JLP_WT

    Plasmid

    #208770

    Purpose

    Tetracycline inducible expression vector for HA-tagged wild-type JLP protein (HA-JLP_WT), used as a donor plasmid for HA-JLP_WT knock-in at the human ROSA26 locus

    Depositor

    Katsuji Yoshioka

    Article

    Suzuki et al Genes Cells. 2023 Nov 14. doi: 10.1111/g

    Insert

    JLP (SPAG9 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCFD5-U6-3-t-attP40

    Plasmid

    #133561

    Purpose

    gRNA vector for targeting near the attP40 locus, use with pHD-3XP3-dsRed-DattP-CRISPR-donor-attP40 based constructs

    Depositor

    Tom Clandinin

    Article

    Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

    Insert

    attP40 region guide RNAs
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    U6

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV ORANGE Gria1-HaloTag KI

    Plasmid

    #139655

    Purpose

    Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and HaloTag donor
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge