We narrowed to 2,956 results for: NAK

1. pMT692 ORF2p-3C-3xF (1-1275, ORFeus-Hs) in pDARMO-PolH2.1

   Plasmid

   #213025

   Purpose

   Expresses full length human LINE-1 ORF2p Core (1-1275) with a C-terminal 3xFlag tag for baculovirus production in insect cells

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   LINE-1 ORF2p (1-1275)
   

   Use

   Tags

   3C-3xFlag (ORF2p)

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-SARS2SΔC-H2gp41

   Plasmid

   #170389

   Purpose

   Generation of SARS-CoV-2 spike pseudotyped lentivirus

   Depositor

   Nir Hacohen

   Article

   Filbin et al Cell Rep Med. 2021 May 18;2(5):100287. d

   Insert

   SARS-CoV-2 Spike (S SARS-CoV-2)
   

   Use

   Tags

   HIV gp41

   Expression

   Mammalian

   Mutation

   Deletion of C terminal and fusion with H2 from HI…

   Promoter

   CMV

   Available since

   June 23, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. PB-TAC-EB-C5

   Plasmid

   #80485

   Purpose

   piggyBac transposon for dox-inducible expression of the EB-C5 polycistronic reprogramming cassette (with mCherry)

   Depositor

   Knut Woltjen

   Article

   Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

   Insert

   lin-28 homolog A (C. elegans) (Pou5f1, Sox2, Klf4, Myc, Lin28a Mouse)
   

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO

   Available since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMT1093 human L1 EN- (ORF2p E43S, D145N) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

   Plasmid

   #213028

   Purpose

   Expresses endonuclease dead (EN- ORF2p E43S, D145N) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 (ORFeus-Hs codon optimized sequence, EN- E43S D145N) with ORF2-3xFlag
   

   Use

   Tags

   3xFlag (ORF2p)

   Expression

   Mammalian

   Mutation

   E43S D145N double mutant EN- endonuclease catalyt…

   Promoter

   CMV

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMT870 human L1 RT- (ORF2p D702Y) ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro (derivative of pMT646)

   Plasmid

   #213027

   Purpose

   Expresses reverse transcriptase dead (RT- ORF2p D702Y) full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 (ORFeus-Hs codon optimized sequence, RT- D702Y) with ORF2-3xFlag
   

   Use

   Tags

   3xFlag (ORF2p)

   Expression

   Mammalian

   Mutation

   D702Y (RT- reverse transcriptase catalytic dead)

   Promoter

   CMV

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMT647 human L1 ORF2p-3xFlag only (ORFeus-Hs, CMV promoter, monocistronic construct) in pCEP4 Puro (derivative of pMT646)

   Plasmid

   #213029

   Purpose

   Expresses human LINE-1 ORF2 only in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 ORF2p only (monocistronic, codon optimized ORFeus-Hs sequence) with ORF2-3xFlag
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTRIP-SFFV-Hygro-2A-TMRPSS2

   Plasmid

   #170390

   Purpose

   Generation of stable lines to enhance SARS-CoV-2 infectivity in ACE2 expressing cells

   Depositor

   Nir Hacohen

   Article

   Filbin et al Cell Rep Med. 2021 May 18;2(5):100287. d

   Insert

   TMPRSS2 (TMPRSS2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   June 23, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. SHP2(WT)@pLKO-Trex-SBP

   Plasmid

   #85457

   Purpose

   Gene expression

   Depositor

   Darrin Stuart

   Article

   Chen et al Nature. 2016 Jul 7;535(7610):148-52.

   Insert

   SHP2 (PTPN11 Human)
   

   Use

   Lentiviral

   Tags

   SBP Tag

   Expression

   Mammalian

   Mutation

   Promoter

   RSV

   Available since

   Feb. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SHP2(C459S)@pLKO-Trex-HA

   Plasmid

   #85459

   Purpose

   Expresses SHP2 mutant in mammalian cells

   Depositor

   Darrin Stuart

   Article

   Chen et al Nature. 2016 Jul 7;535(7610):148-52.

   Insert

   SHP2 (PTPN11 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   C459S

   Promoter

   RSV

   Available since

   Feb. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

10. SHP2(T253M/Q257L)@pLKO-Trex-SBP

    Plasmid

    #85458

    Purpose

    SHP2 mutant gene expression

    Depositor

    Darrin Stuart

    Article

    Chen et al Nature. 2016 Jul 7;535(7610):148-52.

    Insert

    SHP2 (PTPN11 Human)
    

    Use

    Lentiviral

    Tags

    SBP Tag

    Expression

    Mammalian

    Mutation

    T253M/Q257L

    Promoter

    RSV

    Available since

    Feb. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPB-C6F/TdTomato

    Plasmid

    #140826

    Purpose

    OCT4, SOX2, KLF4, C-MYC, KLF2, NANOG (C6F) and tdTomato under the control of TRE (Tet-ON)

    Depositor

    Hideyuki Okano

    Article

    Kisa et al Stem Cell Reports. 2017 Dec 12;9(6):1825

    Inserts

    OCT4 (POU5F1 Human)
    SOX2 (SOX2 Human)
    KLF4 (KLF4 Human)
    C-MYC (MYC Human)
    KLF2 (KLF2 Human)
    NANOG (NANOG Human)
    tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L104P- please see depositor comments

    Promoter

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pETCON-IgG1/design_50

    Plasmid

    #30301

    Depositor

    David Baker

    Article

    Fleishman et al J Mol Biol. 2011 Sep 29.

    Insert

    IgG Fc Designed Binder IgG1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 8, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pETCON-HB20/design_70

    Plasmid

    #30254

    Depositor

    David Baker

    Article

    Fleishman et al J Mol Biol. 2011 Sep 29.

    Insert

    SC1918/H1 HA designed binding protein HB20
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 10, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pETCON-HB28/design_25

    Plasmid

    #30260

    Depositor

    David Baker

    Article

    Fleishman et al J Mol Biol. 2011 Sep 29.

    Insert

    SC1918/H1 HA designed binding protein HB28
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pETCON-E43/design_55

    Plasmid

    #30237

    Depositor

    David Baker

    Article

    Fleishman et al J Mol Biol. 2011 Sep 29.

    Insert

    Mtb ACP2 designed binding protein E43
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pETCON-HB14/design_53

    Plasmid

    #30249

    Depositor

    David Baker

    Article

    Fleishman et al J Mol Biol. 2011 Sep 29.

    Insert

    SC1918/H1 HA designed binding protein HB14
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pETCON-HB50/design_43

    Plasmid

    #30270

    Depositor

    David Baker

    Article

    Fleishman et al J Mol Biol. 2011 Sep 29.

    Insert

    SC1918/H1 HA designed binding protein HB50
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pETCON-HB56/design_34

    Plasmid

    #30276

    Depositor

    David Baker

    Article

    Fleishman et al J Mol Biol. 2011 Sep 29.

    Insert

    SC1918/H1 HA designed binding protein HB56
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pETCON-HB4/design_2

    Plasmid

    #30271

    Depositor

    David Baker

    Article

    Fleishman et al J Mol Biol. 2011 Sep 29.

    Insert

    SC1918/H1 HA designed binding protein HB4
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 16, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pETCON-HB51/design_77

    Plasmid

    #30272

    Depositor

    David Baker

    Article

    Fleishman et al J Mol Biol. 2011 Sep 29.

    Insert

    SC1918/H1 HA designed binding protein HB51
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 16, 2011

    Availability

    Academic Institutions and Nonprofits only