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We narrowed to 5,523 results for: golden gate

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Showing: 2761 - 2780 of 5523 results

  1. L4860 VEGFR2 NTEVp chain (75S NTEVp mutant), VEGFR1 CTEVp chain with ZF6a in MGEV

    Plasmid
    #244188
    Purpose
    Multigene vector for constitutive expression of mNeonGreen, VEGFR2 NTEVp chain (75S NTEVp mutant), and VEGFR1 CTEVp chain with ZF6a
    Depositor
    Joshua Leonard
    Publication
    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158
    Insert
    mNeonGreen; VEGFR2 NTEVp chain with NTEVp 75S mutant; VEGFR1 CTEVp chain with ZF6 synTF
    Use
    Synthetic Biology
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    NTEVp_75S, CTEVp_190K
    Promoter
    hEF1a, CMV
    Available since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244188-plasmid-map-sequence-id-489497

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  4. pMV_AA098

    Plasmid
    #245320
    Purpose
    Cas9 CRISPRko all-in-one positive control; targets CD47
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD47 (CD47 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245320-plasmid-map-sequence-id-488052

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  5. pRDA_835

    Plasmid
    #245327
    Purpose
    Cas9 CRISPRko positive control guide; targets CD59
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD59 (CD59 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245327-plasmid-map-sequence-id-488049

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  6. pMV_AA101

    Plasmid
    #245323
    Purpose
    Cas9 CRISPRa positive control guide; targets CD274
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD274 (CD274 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245323-plasmid-map-sequence-id-488047

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  7. pMV_AA104

    Plasmid
    #245326
    Purpose
    Cas9 CRISPRa positive control guide; targets CD4
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD4 (CD4 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245326-plasmid-map-sequence-id-488034

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  8. pMV_AA102

    Plasmid
    #245324
    Purpose
    Cas9 CRISPRa positive control guide; targets CD47
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD47 (CD47 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245324-plasmid-map-sequence-id-488026

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  9. pRDB_248

    Plasmid
    #245335
    Purpose
    Cas12a CRISPRko positive control guide; targets CD63
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD63 (CD63 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245335-plasmid-map-sequence-id-488021

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  10. MoClo Yeast Secretion Biosensor (YSB) Toolkit

    Plasmid kit
    #1000000268
    Purpose
    Library of plasmids containing a variety of signal peptides, C-tags, and alpha-mating factor tags for use in protein secretion optimization in yeast.
    Depositor
    Joseph Brock
    Application
    Cloning and Synthetic Biology
    Vector type
    Yeast Expression
    Cloning type
    Golden Gate (MoClo)
    Available since
    Sept. 17, 2025
    Availability
    Academic Institutions and Nonprofits only

  14. pBD_PYR1_156-F1

    Plasmid
    #241286
    Purpose
    Backbone for PYR1 sensor library assembly from oligopools; stop codon removed; PYR1 sensor library assembly from oligopools;
    Depositor
    Sean Cutler
    Type
    Empty backbone
    Expression
    Yeast
    Available since
    Aug. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    241286-plasmid-map-sequence-id-484590

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  15. pSEVAb827

    Plasmid
    #217500
    Purpose
    pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb82 backbone, RK2 ori
    Depositor
    Nico Claassens
    Publication
    van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021
    Insert
    superfolder green fluorescent protein (sfGFP)
    Use
    Synthetic Biology
    Mutation
    S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…
    Available since
    July 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    217500-plasmid-map-sequence-id-455513

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  16. pSEVAb837

    Plasmid
    #217501
    Purpose
    pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb83 backbone, pBBR1 ori
    Depositor
    Nico Claassens
    Publication
    van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021
    Insert
    superfolder green fluorescent protein (sfGFP)
    Use
    Synthetic Biology
    Mutation
    S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…
    Available since
    July 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    217501-plasmid-map-sequence-id-444290

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  18. Tad-CBE(TCG2)

    Plasmid
    #235068
    Purpose
    Expresses Tad-CBE(TCG2) in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE(TCG2)-SpCas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, bpNLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235068-plasmid-map-sequence-id-467755

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  19. Tad-CBE(CCA3)

    Plasmid
    #235070
    Purpose
    Expresses Tad-CBE(CCA3) in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE(CCA3)-SpCas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, bpNLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235070-plasmid-map-sequence-id-466792

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  20. Tad-CBE(CCC2)

    Plasmid
    #235072
    Purpose
    Expresses Tad-CBE(CCC2) in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE(CCC2)-SpCas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, bpNLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235072-plasmid-map-sequence-id-469791

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Showing: 2761 - 2780 of 5523 results