We narrowed to 5,185 results for: golden gate
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Plasmid#227909PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertGPR107 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML359_CAPRIN1-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227916PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertCAPRIN1 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML346_VPS35-C-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227891PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertVPS35 homology arms with mEGFP-3xHA for insertion at C terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML307_SEC61B-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227894PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertSEC61B homology arms with mEGFP-3xHA for insertion at N terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML383_CCDC47-C-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227896PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertCCDC47 homology arms with mEGFP-3xHA for insertion at C terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML348_ATG101-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227900PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertATG101 homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML460_MAP1LC3B-N-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227901PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertMAP1LC3B homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML317_COPE-C-term_XTEN80-mEGFP-3xHA_pTMPLT
Plasmid#227905PurposeHomology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.DepositorInsertCOPE homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
UseCRISPRAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKSEe401R
Plasmid#218532PurposeCRISPR/Cas9 genome editing vector pKSEe401R contains the maize codon-optimized Cas9 (zCas9) enhanced by the 5′-UTR fragment of OsMac3 and the pAtUBQ10-DsRED1-NOSt screening cassette in the backbone.DepositorTypeEmpty backboneExpressionPlantAvailable SinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
lenti_hEF1a_rtTA-2A-SOX2-2A-KLF4-2A-cMYC
Plasmid#229545PurposeLentiviral construct to express rtTA and SKM proteinsDepositorInsertrTetR-NLS-VP64-P2A-Sox2-T2A-Klf4-E2A-Myc (SOX2 Human)
UseLentiviralExpressionMammalianPromoterhEF1aAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTBL3328 PB-sfGFP-WPRE-target
Plasmid#226676PurposePiggyBac cargo vector encoding sfGFP followed by WPRE and a transcribed peCHYRON target site.DepositorInsertsfGFP-WPRE-site3target
ExpressionMammalianPromoterEF1alphaAvailable SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVP183
Plasmid#222050PurposeGreenGate DF bridge module containing filler sequence.DepositorInsertFiller
UseSynthetic Biology; Greengate compatible cloning v…Available SinceDec. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVP077
Plasmid#222029PurposeEmpty Kanamycin resistant GreenGate destination vector based on pGGP-AG. Domesticated of Esp3I sites in the backbone and chloramphenicol resistance gene.DepositorTypeEmpty backboneUseSynthetic Biology; Greengate compatible cloning v…MutationAll Esp3i sites domesticatedAvailable SinceDec. 16, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCHW00016
Plasmid#222447PurposeLevel 0, C terminal tag module (TTCG-GCTT) containing p65AD-NLS.DepositorInsertp65AD-NLS
UseSynthetic BiologyExpressionPlantMutationnoAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHW00019
Plasmid#222448PurposeLevel 0, C terminal tag module (TTCG-GCTT) containing GAL4AD-NLS.DepositorInsertGAL4AD-NLS
UseSynthetic BiologyExpressionPlantMutationnoAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanIII
Plasmid#225075PurposeExpresses precursor peptide and lanthipeptide synthetase from LanIII (FAST-RiPPs) in E. coliDepositorInsertLanA (LanIII)
TagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMulti_S_ccdB
Plasmid#223853PurposeGEC-acceptor for 4G-cloning, designed for expression in E. coli (Streptomycin resistance)DepositorTypeEmpty backboneUseGolden-gate acceptorExpressionBacterialAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGGA016
Plasmid#228231PurposeLevel 0 for 5xOp (GreenGate A module)DepositorInsert5xOp
UseSynthetic BiologyAvailable SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR-mTagBFP2
Plasmid#228596PurposeMammalian expression of mTagBFP2, for MuSIC barcode constructionDepositorInsertmTagBFP2
ExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable SinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR-mClover3
Plasmid#228605PurposeMammalian expression of mClover3, for MuSIC barcode constructionDepositorInsertmClover3
ExpressionMammalianPromoterCMVAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only