Addgene

We narrowed to 357 results for: NS1

Showing: 261 - 280 of 357 results

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pB.GFP-KIF1A tail 395-1698

Plasmid

#134618

Purpose

Mammalian expression of mouse KIF1A 395-1698

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF1A (Kif1a Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 394 N-terminal residues.

Promoter

Chicken Beta actin

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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eGFP-KIF5B-Rigor

Plasmid

#172204

Purpose

Expresses KIF5B G234A rigor mutant tagged with eGFP in mammalian cells

Depositor

Juan Bonifacino

Article

Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

Insert

KIF5B (Kif5b Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pB80-hsKIF5B(1-560)-L-GFP

Plasmid

#193716

Purpose

Expression of GFP tagged human kinesin-1 (KIF5B aa1-560)

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

hsKIF5B(1-560)-L-GFP (KIF5B Human, Synthetic)

Use

Tags

EGFP

Expression

Mammalian

Mutation

hsKIF5B(aa1-560);

Promoter

Chicken beta-actin

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-PQBP1

Plasmid

#25804

Depositor

Marius Sudol

Article

Tapia et al J Biol Chem. 2010 Apr 21. ():.

Insert

PQBP1 (PQBP1 Human)

Use

Tags

6xHA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
pSP64-hKCNQ1

Plasmid

#53048

Purpose

Expresses alpha subunit for hKCNQ1 channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Sanguinetti et al Nature. 1996 Nov 7;384(6604):80-3.

Insert

Potassium voltage-gated channel subfamily KQT member 1 (KCNQ1 Human)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

Promoter

SP6

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5B 376-963

Plasmid

#134612

Purpose

Mammalian expression of mouse KIF5B 376-963

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF5B (Kif5b Mouse)

Use

Tags

Halo

Expression

Mammalian

Mutation

WT with deletion of the 375 N-terminal residues.

Promoter

Chicken Beta actin

Available since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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mCardinal-Tensin1-N-18

Plasmid

#56175

Purpose

Localization: Focal Adhesions, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Tensin1 (TNS1 Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pBa.GFP-KIF3B tail 391-747

Plasmid

#134615

Purpose

Mammalian expression of mouse KIF3B 391-747

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF3B (Kif5b Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 390 N-terminal residues.

Promoter

Chicken Beta actin

Available since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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Kif1A(1-489)-DHFR-myc

Plasmid

#117833

Purpose

Constitutively active Kinesin-3 motor tagged with DHFR and myc

Depositor

Thomas Schwarz

Article

Gutnick et al Nat Cell Biol. 2019 Jun;21(6):768-777. d

Insert

Kif1A (1-489) (Kif1a Mouse)

Use

Tags

DHFR and myc

Expression

Mutation

only aa 1-489 (motor domain)

Promoter

CMV

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCA528 huPQBP1

Plasmid

#217449

Purpose

Expresses full-length human PQBP1 from a His-SUMO bacterial expression vector

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

PQBP1 (PQBP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pBetaActin-KIF1aMotor-mScarlet-FKBP

Plasmid

#191336

Purpose

Expresses the fluorescently labeled plus-end directed KIF1a motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

KIF1aMotor-mScarlet-FKBP (Kif1a Mouse)

Use

Tags

Expression

Mammalian

Mutation

motor domain aa 1-405

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-Kif5b-T92N-Myc

Plasmid

#186615

Purpose

Third generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform B Rigor mutant (T92N) fused to Myc tag

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Kinesin Heavy Chain isoform 5b Rigor mutant (T92N) (KIF5B Human)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Mutation

T92N

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-HA-ERK2 GOF

Plasmid

#53200

Purpose

Expresses GST and HA tagged human ERK2 R67S D321N from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Human)

Use

Tags

GST and HA

Expression

Bacterial

Mutation

R67S/D321N

Promoter

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
3xFlag-tdMCP-Erk2 (pErk reporter)

Plasmid

#226167

Purpose

Used to transiently express the pErk reporter in LABEL-seq MAPK activity assays.

Depositor

Dustin Maly

Article

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Insert

3xFlag-tdMCP-Erk2 (MAPK1 Human)

Use

Tags

3xFlag-tdMCP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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mCh-KIF5B*-strep

Plasmid

#120164

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF5B-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 5B (Kif5b Mouse)

Use

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF5B: 1-555 aa

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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HA-KIF5B*-strep

Plasmid

#120170

Purpose

Expresses a chimera of HA tag, a KIF5B-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 5B (Kif5b Mouse)

Use

Tags

HA and Streptavidin

Expression

Mammalian

Mutation

Truncated KIF5B: 1-555

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX4T3-HA-ERK2 R67S

Plasmid

#53201

Purpose

Expresses GST and HA tagged human ERK2 R67S from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK2 (MAPK1 Human)

Use

Tags

GST and HA

Expression

Bacterial

Mutation

R67S

Promoter

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pB80-hsKIF5B(1-560)Rigor-L-2xHA

Plasmid

#193718

Purpose

Expression of HA tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubules

Depositor

Lukas Kapitein

Article

Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

Insert

hsKIF5B(1-560)Rigor-L-2xHA (KIF5B Human, Synthetic)

Use

Tags

2xHA

Expression

Mammalian

Mutation

hsKIF5B(aa1-560), RIGOR: G234A

Promoter

Chicken beta-actin

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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mCh-KIF1A*-strep

Plasmid

#120165

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF1A-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 1A (Kif1a Mouse)

Use

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF1A: 1-405 aa

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPE

Plasmid

#15284

Depositor

Ron Vale

Article

Navone et al J Cell Biol. 1992 Jun . 117(6):1263-75.

Insert

ubiquitous kinesin heavy chain (KIF5B Human)

Use

Tags

Expression

Bacterial

Mutation

The insert is missing the 1st 180 bp of 5' u…

Promoter

Available since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only