We narrowed to 9,533 results for: try

1. CROPseq-multi-v2-Zeo-BsmBI_entry (pRW1500)

   Plasmid

   #225753

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with Zeocin resistance; v2 is compatible with T7 in vitro transcription detection

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJRH-1362 scFv entry plasmid

   Plasmid

   #201912

   Purpose

   Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Insert

   Stuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pShHELIX(Cas12k-TnsC)-sgRNA_entry (CJT113)

   Plasmid

   #181792

   Purpose

   Expresses 3-component ShHELIX containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   nAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMJ204: pU6-SpuFz1_omegaRNA(350nt)-entry

   Plasmid

   #205268

   Purpose

   Human expression vector for SpuFz1 omegaRNA 5' +275 nt extended scaffold (BbsI guide entry site)

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   SpuFz1 omegaRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pShHELIX(Cas12k-TniQ)-sgRNA_entry (CJT111)

   Plasmid

   #181790

   Purpose

   Expresses 3-component ShHELIX containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pShCAST(Cas12k-TnsC)-sgRNA_entry (CJT28)

   Plasmid

   #181789

   Purpose

   Expresses 3-component ShCAST containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   ShTnsB, ShTniQ, ShCas12k-ShTnsC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMJ162: pU6-SpuFz1_omegaRNA(75nt)-entry

   Plasmid

   #205261

   Purpose

   Human expression vector for SpuFz1 omegaRNA 75-nt scaffold (BbsI guide entry site)

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   SpuFz1 omegaRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pShCAST(Cas12k-TniQ)-sgRNA_entry (CJT11)

   Plasmid

   #181787

   Purpose

   Expresses 3-component ShCAST containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   ShTnsB, ShTnsC, ShCas12k-ShTniQ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBacMam2-DiEx-LIC-C entry vector

   Plasmid

   #111752

   Purpose

   Intermediate vector containing HTT gene, except for polyQ region. Used to clone various polyQ lengths into HTT sequence with C-term FLAG tag. Baculovirus transfer vector for insect and mammalian cells

   Depositor

   Cheryl Arrowsmith

   Article

   Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

   Type

   Empty backbone

   Use

   Baculovirus expression

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEntry Tshld(FKBP F36V)-GFP

    Plasmid

    #53260

    Purpose

    pEntry Tshld(FKBP F36V)-GFP is a product of BP recombination reaction between attB flanked Tshld(F36V) system-encoded PCR product and pDonor 201 plasmid.

    Depositor

    Matthew Pratt

    Article

    Lin et al Chembiochem. 2014 Mar 11. doi: 10.1002/c

    Insert

    Tshld(FKBP F36V)-GFP
    

    Use

    Tags

    EGFP, Flag, and HA

    Expression

    Mutation

    FKBP F36V

    Promoter

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-pCamKII-Cre-pU6-SpCas9gRNAentry

    Plasmid

    #210391

    Purpose

    AAV plasmid encoding the CamKII promoter driving Cre expression, along with SpCas9 gRNA entry cassette (RFP dropout)

    Depositor

    Benjamin Kleinstiver

    Article

    Huynh et al Nature. 2024 Nov;635(8037):168-177. doi:

    Insert

    AAV-(ITR)-pCamKII-NLS-Cre-WPRE-bGHpA-(rev)-SpCas9_gRNA-BsmBI_RFPcassette-hU6-(ITR) (NJA158)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CamKII promoter for Cre, human U6 promoter for Sp…

    Available since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV6-Entry-EPHX1c.337T>C-Flag

    Plasmid

    #169169

    Purpose

    Expresses EPHX1 c.337T>C in mammalian cells

    Depositor

    Jeremie Gautheron, Bruno Feve

    Article

    Gautheron et al Elife. 2021 Aug 3;10. pii: 68445. doi: 1

    Insert

    epoxide hydrolase 1 (EPHX1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    c.337T>C

    Promoter

    CMV

    Available since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV6-Entry-EPHX1c.416A>G-Flag

    Plasmid

    #169170

    Purpose

    Expresses EPHX1 c.416A>G in mammalian cells

    Depositor

    Jeremie Gautheron, Bruno Feve

    Article

    Gautheron et al Elife. 2021 Aug 3;10. pii: 68445. doi: 1

    Insert

    epoxide hydrolase 1 (EPHX1 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    c.337T>C

    Promoter

    CMV

    Available since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV6-Entry-EPHX1c.997A>C-Flag

    Plasmid

    #169171

    Purpose

    Expresses EPHX1 c.997A>C in mammalian cells

    Depositor

    Jeremie Gautheron, Bruno Feve

    Article

    Gautheron et al Elife. 2021 Aug 3;10. pii: 68445. doi: 1

    Insert

    epoxide hydrolase 1 (EPHX1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    c.997A>C

    Promoter

    CMV

    Available since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV6-Entry-EPHX1c.1212G>C-Flag

    Plasmid

    #169172

    Purpose

    Expresses EPHX1 c.1212G>C in mammalian cells

    Depositor

    Jeremie Gautheron, Bruno Feve

    Article

    Gautheron et al Elife. 2021 Aug 3;10. pii: 68445. doi: 1

    Insert

    epoxide hydrolase 1 (EPHX1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    c.1212G>C

    Promoter

    CMV

    Available since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV6-Entry-EPHX1c.1288G>C-Flag

    Plasmid

    #169173

    Purpose

    Expresses EPHX1 c.1288G>C in mammalian cells

    Depositor

    Jeremie Gautheron, Bruno Feve

    Article

    Gautheron et al Elife. 2021 Aug 3;10. pii: 68445. doi: 1

    Insert

    epoxide hydrolase 1 (EPHX1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    c.1288G>C

    Promoter

    CMV

    Available since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBacMam2-DiEx-LIC-N entry vector

    Plasmid

    #111751

    Purpose

    Intermediate vector containing HTT gene, except for polyQ region. Used to clone various polyQ lengths into HTT sequence with N-term FLAG tag. Baculovirus transfer vector for insect and mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Type

    Empty backbone

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. YCe2764 HC_Kan_RFP-Connector entry vector

    Plasmid

    #100636

    Purpose

    EMMA toolkit connector-entry vector

    Depositor

    Yizhi Cai

    Article

    Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. CROPseq-multi-v2-palmitoyl-mTagBFP2-BsmBI_entry (pRW1509)

    Plasmid

    #225755

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with membrane-localized palmitoyl-mTagBFP2; v2 is compatible with T7 in vitro transcription detection

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CROPseq-multi-v2-mTagBFP2-NLS-BsmBI_entry (pRW1506)

    Plasmid

    #225754

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with nuclear-localized mTagBFP2-NLS; v2 is compatible with T7 in vitro transcription detection

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge