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Dual modes of CRISPR-associated transposon homing.
Saito M, Ladha A, Strecker J, Faure G, Neumann E, Altae-Tran H, Macrae RK, Zhang F
Cell. 2021 Apr 29;184(9):2441-2453.e18. doi: 10.1016/j.cell.2021.03.006. Epub 2021 Mar 25. PubMed Article

Plasmids from Article

ID Plasmid Purpose
168133pMS1: pHelper(AvCAST)_AvPSP1Inducible expression of AvCAST proteins with crRNA targeting AvPSP1.
168134pMS2: pHelper(AvCAST)_AvPSP1_ΔTniQInducible expression of AvCAST proteins (except TniQ) with crRNA targeting AvPSP1.
168135pMS3: pHelper(AvCAST)_AvPSP1_ΔTnsDInducible expression of AvCAST proteins (except TnsD) with crRNA targeting AvPSP1.
168137pMS4: pHelper(AvCAST)_entryInducible expression of AvCAST proteins. Two BsaI sites for spacer cloning.
168138pMS5: pHelper(AvCAST)_entry_ΔTniQInducible expression of AvCAST proteins (except TniQ). Two BsaI sites for spacer cloning.
168139pMS6: pHelper(AvCAST)_entry_ΔTnsDInducible expression of AvCAST proteins (except TnsD). Two BsaI sites for spacer cloning.
168140pMS7: pCascade(AvCAST)_entryInducible expression of AvCAST Cascade proteins. Two BsaI sites for spacer cloning.
168141pMS8: pTns(AvCAST)Inducible expression of AvCAST TnsA, TnsB, TnsC, TniQ and TnsD proteins.
168142pMS9: pTns(AvCAST)_ΔTniQInducible expression of AvCAST TnsA, TnsB, TnsC and TnsD proteins.
168143pMS10: pTns(AvCAST)_ΔTnsDInducible expression of AvCAST TnsA, TnsB, TnsC and TniQ proteins.
168144pMS11: pTns(AvCAST)_ΔTniQ,ΔTnsDInducible expression of AvCAST TnsA, TnsB and TnsC proteins.
168145pMS12: pDonor(AvCAST)AvCAST donor harboring non-functional cargo.
168146pMS13: pTarget(AvCAST)Target plasmid containing AvPSP1 and attachement site for AvCAST.
168147pMS14: pTwinStrep-SUMO-AvTnsAAvTnsA expression construct for protein purification.
168148pMS15: pTwinStrep-SUMO-AvTnsBAvTnsB expression construct for protein purification.
168149pMS16: pTwinStrep-SUMO-AvTnsCAvTnsC expression construct for protein purification.
168150pMS17: pTwinStrep-SUMO-AvTnsDAvTnsD expression construct for protein purification.
168151pMS18: pHelper(PmcCAST)_PmcPSP1Inducible expression of PmcCAST proteins with crRNA targeting PmcPSP1.
168152pMS19: pHelper(PmcCAST)_PmcPSP1_ΔTniQInducible expression of PmcCAST proteins (except TniQ) with crRNA targeting PmcPSP1.
168153pMS20: pHelper(PmcCAST)_PmcPSP1_ΔTnsDInducible expression of PmcCAST proteins (except TnsD) with crRNA targeting PmcPSP1.
168154pMS21: pHelper(PmcCAST)_entryInducible expression of PmcCAST proteins. Two BsaI sites for spacer cloning.
168155pMS22: pHelper(PmcCAST)_entry_ΔTniQInducible expression of PmcCAST proteins (except TniQ). Two BsaI sites for spacer cloning.
168156pMS23: pHelper(PmcCAST)_entry_ΔTnsDInducible expression of PmcCAST proteins (except TnsD). Two BsaI sites for spacer cloning.
168157pMS24: pCascade(PmcCAST)_entryInducible expression of PmcCAST Cascade proteins. Two BsaI sites for spacer cloning.
168158pMS25: pTns(PmcCAST)Inducible expression of PmcCAST TnsAB, TnsC, TniQ and TnsD proteins.
168159pMS26: pTns(PmcCAST)_ΔTniQInducible expression of PmcCAST TnsAB, TnsC and TnsD proteins.
168160pMS27: pTns(PmcCAST)_ΔTnsDInducible expression of PmcCAST TnsAB, TnsC and TniQ proteins.
168161pMS28: pTns(PmcCAST)_ΔTniQ,ΔTnsDInducible expression of PmcCAST TnsAB and TnsC proteins.
168162pMS29: pDonor(PmcCAST)PmcCAST donor harboring non-functional cargo.
168163pMS30: pTarget(PmcCAST)Target plasmid containing PmcPSP1 and attachment site for PmcCAST.
168164pMS31: pTwinStrep-SUMO-PmcTnsABPmcTnsAB expression construct for protein purification.
168165pMS32: pTwinStrep-SUMO-PmcTnsCPmcTnsC expression construct for protein purification.
168166pMS33: pTwinStrep-SUMO-PmcTnsDPmcTnsD expression construct for protein purification.

Antibodies from Article