We narrowed to 48,727 results for: INA

1. pAAV-EF1a-CDreV

   Plasmid

   #140133

   Purpose

   can be used to generate AAV virus that will express fusion protein of split Dre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer

   Depositor

   Hongkui Zeng

   Article

   Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

   Insert

   CDreV
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEGFPC2-gephyrin S268A/270A

   Plasmid

   #199608

   Purpose

   Encodes N-terminal eGFP-tagged P1-gephyrin S268/270A for expression in mammalian cells.

   Depositor

   Shiva Tyagarajan

   Article

   Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

   Insert

   Gephyrin (Gphn Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S268A/S270A

   Promoter

   CMV

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEM BirA-2A-Citrine-SV40pA-FRT-Kan-FRT

   Plasmid

   #89890

   Purpose

   BAC donor construct containing 3XHA-tagged BirA, a ribosome skipping motif - 2A, Citrine reporter, polyadenyation signal, followed by FRT recombination sites flanking kanamycin selection cassette.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

   Insert

   HA-BirA-2A-citrine
   

   Use

   Unspecified

   Tags

   Citrine

   Expression

   Mutation

   Promoter

   Available since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. t1-400

   Plasmid

   #166577

   Purpose

   For expression of human talin head (residues 1-400) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.

   Depositor

   Vesa Hytönen

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2020 Dec 22;11

   Insert

   Tln1 Head (1-400) (TLN1 Human)
   

   Use

   Tags

   His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

   Expression

   Bacterial

   Mutation

   residues 1-400 only

   Promoter

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSems meGFP-farnesyl

   Plasmid

   #222949

   Purpose

   Expression of C-terminal fragment of HRAS responsible for farnesylation tagged with GFP in mammalian cells

   Depositor

   Jacob Piehler

   Article

   Winkelmann et al Nat Commun. 2024 Jul 10;15(1):5813. doi:

   Insert

   C-terminal fragment of HRAS (170-189) (HRAS Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   C-terminal fragment of HRAS (170-189)

   Promoter

   CMV

   Available since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CAGGS-safb∆sap-NLS-3XFLAG-V5

   Plasmid

   #196091

   Purpose

   Expresses mouse SAFB lacking the n-terminal SAP domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

   Depositor

   Mauro Calabrese

   Article

   Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

   Insert

   SAFB (Safb Mouse)
   

   Use

   Tags

   C-terminal 3xFLAG and C-terminal V5

   Expression

   Bacterial and Mammalian

   Mutation

   Removed amino acids 31-65 (SAP domain) of mouse S…

   Promoter

   CAGGS

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFPC2-gephyrin S268E/270E

   Plasmid

   #199609

   Purpose

   Encodes N-terminal eGFP-tagged P1-gephyrin S268/270E for expression in mammalian cells.

   Depositor

   Shiva Tyagarajan

   Article

   Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

   Insert

   Gephyrin (Gphn Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S268E/S270E

   Promoter

   CMV

   Available since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-N-Flag-ASC1

   Plasmid

   #75134

   Purpose

   Expresses N-terminal Flag-tagged ASC1 in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   PYD and CARD domain containing (Pycard Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   F23S (please see depositor comments below)

   Promoter

   CMV

   Available since

   Aug. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDEST53-LRRK2-3XKD

   Plasmid

   #25049

   Depositor

   Mark Cookson

   Article

   Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   "Kinase Dead" Lysine 1906 mutated to A…

   Promoter

   Available since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pAcBac1-Mb-sfGFP 150TAG

    Plasmid

    #197570

    Purpose

    Expression of sfGFP 150TAG with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    sfGFP 150TAG
    Pyl-tRNA (4 copies)
    

    Use

    Tags

    V5-His6

    Expression

    Mammalian

    Mutation

    N150TAG

    Promoter

    CMV and U6/H1

    Available since

    April 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-N-Flag-NLRP3 1-90

    Plasmid

    #75137

    Purpose

    Expresses N-terminal Flag-tagged amino acids 1-90 of NLRP3 (the pyrin domain) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    fragment encoding amino acids 1-90

    Promoter

    CMV

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAGGS-Twin-Strep-TEV-DGKζ

    Plasmid

    #202531

    Purpose

    Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human diacylglycerol kinase ζ (DGKζ) in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Insert

    DGKZ (DGKZ Human)
    

    Use

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and chicken β-actin promoter

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-EF1a-CCreV

    Plasmid

    #140132

    Purpose

    can be used to generate AAV virus that will express fusion protein of split Cre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer

    Depositor

    Hongkui Zeng

    Article

    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

    Insert

    CCreV
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-EF1a-NCreV

    Plasmid

    #140131

    Purpose

    can be used to generate AAV virus that will express fusion protein of split Cre (N-terminal) and fungal photoreceptor Vivid (VVD) monomer

    Depositor

    Hongkui Zeng

    Article

    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

    Insert

    NCreV
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HA-HIF2α P405A Δ450-572-pBabe-Puro

    Plasmid

    #25958

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: Proline 405 to Alanin…

    Promoter

    Available since

    Sept. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDEST53-LRRK2-3XKD-R1441C

    Plasmid

    #25050

    Depositor

    Mark Cookson

    Article

    Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    "Kinase Dead" Lysine 1906 mutated to A…

    Promoter

    Available since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pDEST53-LRRK2-3XKD-Y1699C

    Plasmid

    #25051

    Depositor

    Mark Cookson

    Article

    Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    "Kinase Dead" Lysine 1906 mutated to A…

    Promoter

    Available since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pCMV-pro-Il1b-C-Flag

    Plasmid

    #75131

    Purpose

    Expresses C-terminal flag-tagged pro-IL-1β in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    interleukin 1 beta (Il1b Mouse)
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    (please see depositor comments below)

    Promoter

    CMV

    Available since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-EF1a-iCreV

    Plasmid

    #140135

    Purpose

    Can be used to generate AAV virus that will express light-inducible site-specific iCreV recombinase

    Depositor

    Hongkui Zeng

    Article

    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

    Has Service

    AAV PHP.eB and AAV1

    Insert

    iCreV
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-N-Flag-NLRP3 711-1034

    Plasmid

    #75141

    Purpose

    Expresses N-terminal Flag-tagged amino acids 711-1034 of NLRP3 (the LRR domain) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    fragment encoding amino acids 711-1034

    Promoter

    CMV

    Available since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge