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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,838 results for: codon optimized

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Showing: 2781 - 2800 of 4838 results

  1. pX330-Flag-Sniper SpCas9 (without sgRNA; with silent mutations)

    Plasmid
    #126777
    Purpose
    Expression plasmid for human codon-optimized increased fidelity Sniper SpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    Sniper SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    F539S, M763I, K890N
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126777-plasmid-map-sequence-id-410581

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  3. SpCas9-NRCH-SsAPOBEC3B

    Plasmid
    #198550
    Purpose
    Expresses human codon-optimized SpCas9-NRCH-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NRCH-SsAPOBEC3B-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NRCH-SsAPOBEC3B
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198550-plasmid-map-sequence-id-388894

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  8. pCC_07 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139092
    Purpose
    Expresses human codon-optimized inactive xCas9 3.7 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dxCas9 3.7-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T, R324L,S409I, E480K, E543D, M6…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139092-plasmid-map-sequence-id-273889

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  9. pBT3007

    Plasmid
    #122538
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a early endosome localized gene (rab-5) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_CemOrange2::rab-5 (rab-5 Synthetic, Nematode)
    Expression
    Bacterial
    Promoter
    Pnhx-2
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122538-plasmid-map-sequence-id-238129

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  10. pBT3011

    Plasmid
    #122539
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a lysosomal localized gene (cup-5) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_CemOrange2::cup-5 (cup-5 Synthetic, Nematode)
    Expression
    Bacterial
    Promoter
    Pnhx-2
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122539-plasmid-map-sequence-id-238204

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  11. pBT3014

    Plasmid
    #122540
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a late endosome localized gene (rab-7) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_CemOrange2::rab-7 (rab-7 Synthetic, Nematode)
    Expression
    Bacterial
    Promoter
    Pnhx-2
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122540-plasmid-map-sequence-id-238121

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  12. pBT3019

    Plasmid
    #122541
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a peroxisome target peptide (SKL) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_CemOrange2::SKL
    Tags
    SKL
    Expression
    Bacterial
    Promoter
    Pnhx-2
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122541-plasmid-map-sequence-id-238135

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  13. pBT3024

    Plasmid
    #122542
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a mitochondrial target peptide (mt) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_mt::CemOrange2
    Tags
    MLSLRQSIRFFKPATRTLCSSRTLL
    Expression
    Bacterial
    Promoter
    Pnhx-2
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122542-plasmid-map-sequence-id-238125

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Showing: 2781 - 2800 of 4838 results