We narrowed to 32,111 results for: Eng

1. p3E-U6a-U6c-uqcrc1 guide

   Plasmid

   #107602

   Purpose

   An entry vector with U6a and U6c promoter driving uqcrc1 guide RNAs expression

   Depositor

   Qing Deng

   Article

   Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

   Insert

   uqcrc1 guide RNA sequences
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMV-TRP1

   Plasmid

   #65335

   Purpose

   standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker TRP1 which could be released and ligated to series of TUs

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   TRP1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. Gal4pBS3-Pleum

   Plasmid

   #60330

   Purpose

   Contains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.

   Depositor

   Hal Alper

   Article

   Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

   Insert

   promoter
   

   Use

   Synthetic Biology; Yeast synthetic hybrid promote…

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 11, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. Gal4pBS1-Pleum

   Plasmid

   #59993

   Purpose

   Contains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.

   Depositor

   Hal Alper

   Article

   Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

   Insert

   promoter
   

   Use

   Synthetic Biology; Yeast synthetic hybrid promote…

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 11, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pMXs-B5

   Plasmid

   #31709

   Depositor

   Duanqing Pei

   Article

   Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

   Insert

   miR-363 (Mir363 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pNTPTP alpha (S204A)

   Plasmid

   #17699

   Depositor

   Xinmin Zheng

   Article

   Zheng et al J Biol Chem. 2002 Jun 14. 277(24):21922-

   Insert

   PTPalpha S204A (PTPRA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S204A

   Promoter

   Available since

   April 24, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3.1(+)- e18

   Plasmid

   #131670

   Purpose

   Expresses an engineered variant of RAD18 ("e18") that stimulates CRISPR mediated HDR.

   Depositor

   Alberto Ciccia

   Article

   Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

   Insert

   e18 (engineered RAD18 with SAP domain deleted) (RAD18 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry-pCAG-donor_opt(R2Tg)-GFP(intron)

   Plasmid

   #223249

   Purpose

   Mammalian expression of the optimized R2Tg RNA donor

   Depositor

   Wei Li

   Article

   Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

   Insert

   R2Tg donor_opt
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Mm-PylRS-AF/Pyl-tRNACUA

   Plasmid

   #122650

   Purpose

   tRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.

   Depositor

   Howard Hang

   Article

   Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038

   Insert

   PylRS-AF, Pyl-tRNACUA
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Tyrosine 306 changed to Alanine, Tyrosine 384 cha…

   Promoter

   Available since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDIRECT_22C

    Plasmid

    #91135

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:npt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAVS1-Puro CAG J591-scFV T-CAR

    Plasmid

    #171961

    Purpose

    Donor plasmid for J591 T-CAR expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Harris et al J Immunol Regen Med. 2023 May;20:100074.

    Insert

    Synthetic J591-scFV CAR
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-hsyn-GRAB_DA2m

    Plasmid

    #140553

    Purpose

    Expresses the 2nd generation medium-affinity DA sensor GRAB_DA2m in neurons

    Depositor

    Yulong Li

    Article

    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

    Has Service

    AAV9

    Insert

    The 2nd generation DA sensor GRAB_DA2m (medium affinity)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEGFP-TRIM28

    Plasmid

    #45568

    Depositor

    Fanxiu Zhu

    Article

    Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

    Insert

    TRIM28 (TRIM28 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    G34S mutation in EGFP

    Promoter

    CMV

    Available since

    May 29, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. 6xHsa.NFKB:d2EGFP

    Plasmid

    #239990

    Purpose

    Destabilized green fluorescent protein (d2EGFP) under transcriptional control of NF-kB activity

    Depositor

    Raquel Espin-Palazon

    Insert

    Destabilized EGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    cfos minimal promoter

    Available since

    June 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPRi_Mxi1_yl

    Plasmid

    #91248

    Purpose

    CRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertion

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

    Inserts

    Codon optimized dCas9-Mxi1
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC3.1-caggs-Crimson-CAAX

    Plasmid

    #190041

    Purpose

    Red Fluorescent Protein for Long-Term Labeling of Fine Structures in Neurons

    Depositor

    Michael Lin

    Article

    Ning et al Front Cell Dev Biol. 2022 Jun 29;10:8934

    Insert

    Crimson
    

    Use

    Tags

    CAAX

    Expression

    Mammalian

    Mutation

    Promoter

    caggs

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-TRE-DIO-FLPo

    Plasmid

    #118027

    Purpose

    AAV vector for sparse and bright labeling in a cell-type specific manner

    Depositor

    Minmin Luo

    Article

    Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

    Insert

    TRE-DIO-FLPo
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBBR_Cas9_sp1

    Plasmid

    #154851

    Purpose

    Expresses SpCas9 and sgRNA that targets the upp gene in Rhodobacter sphaeroides genome

    Depositor

    John van der Oost

    Article

    Mougiakos et al Microb Cell Fact. 2019 Nov 25;18(1):204.

    Inserts

    Spcas9 gene
    sgRNA scaffold
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Mutation

    Codon harmonized variant for expression in Rhodob…

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAVS1-Puro CAG PSMA nanobody T-CAR

    Plasmid

    #171962

    Purpose

    Donor plasmid for PSMA nanobody T-CAR expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Harris et al J Immunol Regen Med. 2023 May;20:100074.

    Insert

    Synthetic anti-PSMA nanobody CAR
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-hsyn-GRAB_rDA1h

    Plasmid

    #140557

    Purpose

    Expresses the red fluorescent high-affinity DA sensor GRAB_rDA1h in neurons

    Depositor

    Yulong Li

    Article

    Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

    Has Service

    AAV9

    Insert

    Red fluorescent DA sensor GRAB_rDA1h (high affinity)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge