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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,463 results for: 110

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Showing: 2821 - 2840 of 4463 results

  1. SSTR5-DuET

    Plasmid
    #213380
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Publication
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    SSTR5 (SSTR5 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213380-plasmid-map-sequence-id-430735

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  2. HA-MBP-TEVpCS-ADGRV1 CTFdeltaTA-FLAG

    Plasmid
    #217743
    Purpose
    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains
    Depositor
    Stephen Blacklow
    Publication
    Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ADGRV1 (ADGRV1 Human)
    Tags
    FLAG and HA
    Expression
    Mammalian
    Mutation
    Deletion of residues 1-5896
    Available since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217743-plasmid-map-sequence-id-430305

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  3. hTMEM115-TEV-3xHA-P2A-mScarlet HDR template

    Plasmid
    #214998
    Purpose
    Endogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting.
    Depositor
    Wade Harper
    Publication
    Hollingsworth et al bioRxiv 2024.04.19.590338
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hTMEM115-TEV-3xHA-P2A-mScarlet HDR template (TMEM115 Human)
    Use
    Hdr template
    Tags
    3xHA
    Promoter
    None
    Available since
    April 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    214998-plasmid-map-sequence-id-429346

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  4. hTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template

    Plasmid
    #214999
    Purpose
    Endogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mNG for cell sorting.
    Depositor
    Wade Harper
    Publication
    Hollingsworth et al bioRxiv 2024.04.19.590338
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template (TMEM115 Human)
    Use
    Hdr template
    Tags
    3xHA
    Promoter
    None
    Available since
    April 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    214999-plasmid-map-sequence-id-429345

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  5. pBiT3.1-N_CHAF1B:1-559

    Plasmid
    #211144
    Purpose
    Mammalian expression of full-length human CHAF1B. N-terminal HiBiT tag.
    Depositor
    Cheryl Arrowsmith
    Publication
    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CHAF1B:M1-P559 (CHAF1B Human)
    Tags
    MVSGWRLFKKISGSSGGSSG
    Expression
    Mammalian
    Mutation
    wild type
    Promoter
    CMV
    Available since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211144-plasmid-map-sequence-id-425950

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  7. pmCer-TopBP1

    Plasmid
    #211412
    Purpose
    mammalian expression of human TopBP1 N-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TopBP1 (TOPBP1 Human)
    Tags
    mCerulean
    Expression
    Mammalian
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211412-plasmid-map-sequence-id-420515

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  9. ataxin3:1-431_Q79_BV

    Plasmid
    #209733
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    Ataxin 3 (ATXN3 Human)
    Tags
    6xHis-SBP-TEV
    Expression
    Bacterial
    Promoter
    Polyhedrin
    Available since
    Nov. 13, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    209733-plasmid-map-sequence-id-414202

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  11. TFORF3374

    Plasmid
    #144850
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Publication
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    VAX2 (VAX1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144850-plasmid-map-sequence-id-404414

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  12. TFORF3262

    Plasmid
    #144738
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Publication
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CEBPE (CEBPE Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144738-plasmid-map-sequence-id-404340

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  13. TFORF2280

    Plasmid
    #144332
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Publication
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PRDM2 (PRDM2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144332-plasmid-map-sequence-id-404178

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  14. TFORF2277

    Plasmid
    #144207
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Publication
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    PRDM2 (PRDM2 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144207-plasmid-map-sequence-id-404090

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  15. TFORF1882

    Plasmid
    #142514
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Publication
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SOX30 (SOX30 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    June 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    142514-plasmid-map-sequence-id-397218

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  16. pcDNA3-RIPK3[V458A][Q459A][V460A][G461A]-FLAG

    Plasmid
    #199328
    Purpose
    mutated RHIM motive (interaction inhibited)
    Depositor
    Adrian Press
    Publication
    Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    changed V458, Q459,V460 and G461 to alanine
    Available since
    June 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    199328-plasmid-map-sequence-id-395451

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  17. pcDNA3-RIPK3[S199A][S227A]-FLAG

    Plasmid
    #199323
    Purpose
    double mutated phosphorylation site (kinase acitivity and MLKL interaction inhibited)
    Depositor
    Adrian Press
    Publication
    Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    changed Serine 199 and 227 to Alanine
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    199323-plasmid-map-sequence-id-394156

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  20. SPOCK1_23-439_HisN_AviC

    Plasmid
    #195876
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SPOCK1 (SPOCK1 Human)
    Use
    Pfhmsp-avic-lic-n, baculovirus expression
    Promoter
    Polyhedrin
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195876-plasmid-map-sequence-id-387253

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Showing: 2821 - 2840 of 4463 results