We narrowed to 14,721 results for: FIE

1. PpyCAG-KLF2-IB

   Plasmid

   #60441

   Purpose

   Transient human KLF2 expression

   Depositor

   Austin Smith

   Article

   Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

   Insert

   KLF2 (KLF2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-SpCas9-2A-GFP-noITR

   Plasmid

   #118415

   Purpose

   Cas9 GFP plasmid constitutively expressed from CAG promoter. AAV repeat region removed to increase expression

   Depositor

   Mark Denham

   Article

   Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-EPG

   Plasmid

   #117978

   Purpose

   Express EPG in mamalian cells

   Depositor

   Assaf Gilad

   Article

   Krishnan et al Sci Rep. 2018 Jun 8;8(1):8764. doi: 10.1

   Insert

   Electromagnetic perceptive gene
   

   Use

   Tags

   HIS and v5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMP71-super-IL-2

   Plasmid

   #174599

   Purpose

   Expresses extracellular domain of human IL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F) developed by the Garcia Lab

   Depositor

   Stanley Riddell

   Article

   Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

   Insert

   extracellular domain of huIL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PDIA5-bio-His

   Plasmid

   #52072

   Purpose

   Expresses full-length Protein disulfide-isomerase A5 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   PDIA5 (PDIA5 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLV_hUbC-dSpCas9-2xVP64-T2A-BSD

   Plasmid

   #162333

   Purpose

   Lentiviral expression of dSpCas9-2xVP64 with blasticidin resistance

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   dSpCas9-2xVP64
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A and H840A

   Promoter

   hUbC

   Available since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pX330.sgNf1.4

   Plasmid

   #92028

   Purpose

   pX330 sgNf1.4 for Nf1 deletion

   Depositor

   Wen Xue

   Article

   Song et al Gastroenterology. 2017 Apr;152(5):1161-1

   Insert

   sgNf1.4
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

8. ULBP3-bio-His

   Plasmid

   #51859

   Purpose

   Expresses full-length NKG2D ligand 3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   ULBP3 (ULBP3 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-EF1a-DIO-tdTomato-WPRE

   Plasmid

   #202610

   Purpose

   Double floxed red fluorescent protein tdTomato in AAV production vector expressed under the mammalian promoter (EF1a)

   Depositor

   Francois St-Pierre

   Article

   Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

   Insert

   tdTomato
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSC-PreE2

    Plasmid

    #52264

    Purpose

    Bacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with PreScission protease cleavage site linker

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Insert

    Ubc9 GST fusion (UBE2I Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTet-O-OVOL1-T2A-PuroR

    Plasmid

    #162347

    Purpose

    Lentiviral expression of OVOL1 under the control of the TetON promoter

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    OVOL1 (OVOL1 Human)
    

    Use

    Lentiviral

    Tags

    T2A-PuroR

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-ON

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer

    Plasmid

    #172501

    Purpose

    Encodes the DisCas7-11 full locus along with a DR and golden gate site for cloning spacers

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

    Insert

    DisCas7-11 full locus with Golden gate acceptor site for spacer
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti-PGK-Hygro-amp-sgRNA-resistant PBRM1

    Plasmid

    #107407

    Purpose

    Lentiviral expression of gRNA resistant PBRM1

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    sg resistant PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    sgRNA resistant

    Promoter

    Available since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ATP1A1_G2_Dual_sgRNA

    Plasmid

    #173201

    Purpose

    Coselection for ABE or NHEJ in human cells. Vector for tandem expression of ATP1A1 G2 sgRNA with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G2 sgRNA + user-specified sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLV_hU6-sgRNA_hUbC-GFP-P2A-PuroR

    Plasmid

    #162335

    Purpose

    Lentiviral expression of S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    S. pyogenes sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRSFDuet-1 IntS8 AA 1-308

    Plasmid

    #196905

    Purpose

    Expresses His-tagged Drosophila IntS8 amino acids 1-308

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    Integrator subunit 8 (IntS8 Fly)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLEX Myc-HA

    Plasmid

    #229499

    Purpose

    For lentiviral expression of Myc coding sequence with an HA tag.

    Depositor

    Davide Ruggero

    Article

    Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

    Insert

    MYC
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSC-IntE2

    Plasmid

    #52265

    Purpose

    Bacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with GyrA intein linker

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Insert

    Ubc9 GST fusion (UBE2I Human, Synthetic)
    

    Use

    Tags

    GST and GyrA-intein

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SORT1-bio-His

    Plasmid

    #52024

    Purpose

    Expresses full-length Sortilin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    SORT1 (SORT1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Human NANOS3-P2A-mCherry knock-in targeting construct

    Plasmid

    #59721

    Purpose

    Gene targeting construct for human NANOS3 locus, introducing a P2A-mCherry reporter gene. Contains an frt-PGK-Neo-PA-frt cassette that can be excised.

    Depositor

    Jacob Hanna

    Article

    Irie et al Cell. 2015 Jan 15;160(1-2):253-68. doi:

    Insert

    P2A mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge