We narrowed to 4,454 results for: TIL

1. pBS2E

   Plasmid

   #55169

   Purpose

   Empty vector, integration at lacA, ampr, mlsr

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology; Bacillus biobrick box

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pJMP3

   Plasmid

   #79875

   Purpose

   Bacillus subtilis sgRNA expression vector; integrates into thrC

   Depositor

   Jason Peters, Carol Gross, Stanley Qi

   Article

   Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

   Insert

   sgRNA RR1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   veg

   Available since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)

   Plasmid

   #71719

   Purpose

   Expressing a GFP-tagged degron in the soma of C. elegans

   Depositor

   Abby Dernburg

   Article

   Zhang et al Development. 2015 Nov 9. pii: dev.129635

   Insert

   auxin-responsive protein IAA17 (AXR3 Mustard Weed)
   

   Use

   Tags

   EmGFP

   Expression

   Worm

   Mutation

   minimal degron sequence (71-114aa) with start cod…

   Promoter

   eef-1A.1 (eft-3)

   Available since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJMP2

   Plasmid

   #79874

   Purpose

   Bacillus subtilis sgRNA expression vector; integrates into amyE

   Depositor

   Jason Peters, Carol Gross, Stanley Qi

   Article

   Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

   Insert

   sgRNA RR1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   veg

   Available since

   Sept. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_KanR neo

   Plasmid

   #167901

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Syn_CapChR2_eYFP

   Plasmid

   #188035

   Purpose

   Expresses CapChR2-eYFP in mammalian neurons

   Depositor

   Peter Hegemann

   Article

   Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

   Insert

   CapChR2-eYFP
   

   Use

   AAV

   Tags

   eYFP

   Expression

   Mutation

   S43D, L112C, T139C and N238E mutations in CoChR

   Promoter

   hSyn

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS6-YFP-SMASh(AI)

   Plasmid

   #111500

   Purpose

   Expresses Venus YFP with a C-terminal SMASh(AI) tag fusion in mammalian cells. [AI = asunaprevir inhibited].

   Depositor

   Michael Lin

   Article

   Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi

   Insert

   YFP-SMASh(AI)
   

   Use

   Tags

   HA epitope tag and SMASh tag

   Expression

   Mammalian

   Mutation

   The HCV NS3 protease carries V36M, T54A, and S122…

   Promoter

   CMV

   Available since

   July 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVUHshp53-tTR-KRAB

   Plasmid

   #11654

   Depositor

   Didier Trono, Patrick Aebischer

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   hUbiquitin C, GFP, tTR-KRAB, shRNA against TP53, no tetO (TP53 Human)
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3 HA Arf1 ActQ71L

   Plasmid

   #10832

   Depositor

   Thomas Roberts

   Article

   Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

   Insert

   Arf1 Q71L (ARF1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Q71L. Defective in GTP hydrolysis. Constitutive…

   Promoter

   Available since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFlp-Gm

    Plasmid

    #222335

    Purpose

    Expresses Flp and gentamicin to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-gent-pBBR1-ELT3

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Inserts

    sacB (sacB B. subtilis)
    flp (flp Budding Yeast)
    cI (cI )
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    cl, flp, and sacB

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMuLE ENTR CMV Renilla Luciferase L5-L2

    Plasmid

    #62186

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and renilla luciferase module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    Renilla luciferase
    

    Use

    Luciferase; Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS6-YFP-SMASh(TI)

    Plasmid

    #111501

    Purpose

    Expresses Venus YFP with a C-terminal SMASh(TI) tag fusion in mammalian cells. [TI = telaprevir inhibited].

    Depositor

    Michael Lin

    Article

    Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi

    Insert

    YFP-SMASh(TI)
    

    Use

    Tags

    HA epitope tag and SMASh tag

    Expression

    Mammalian

    Mutation

    The HCV NS3 protease carries F43L, Q80K, S122R, a…

    Promoter

    CMV

    Available since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. aCat TS WT (1-697 mTFP1-TSmod-Venus 698-907)

    Plasmid

    #101299

    Purpose

    FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

    Depositor

    Alpha Yap

    Article

    Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

    Insert

    alpha-Catenin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains amino acids 1-698 and 698-906 of alpha-C…

    Promoter

    Available since

    June 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFlp-Km

    Plasmid

    #222334

    Purpose

    Expresses Flp and kanamycin to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-neo-pBBR1-ELT3

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Inserts

    sacB (sacB B. subtilis)
    flp (flp Budding Yeast)
    cI (cI )
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    cl, flp, and sacB

    Available since

    Aug. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. MYC-hTRAK1

    Plasmid

    #225204

    Purpose

    Expresses MYC-tagged human TRAK1

    Depositor

    Thomas Schwarz

    Article

    Pekkurnaz et al Cell. 2014 Jul 3;158(1):54-68. doi: 10.1

    Insert

    trak1 (TRAK1 Human)
    

    Use

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFNC-3

    Plasmid

    #233296

    Purpose

    Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. CmR, easily curable via sucrose counterselection.

    Depositor

    Pablo Ivan Nikel

    Article

    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

    Inserts

    BxbI phage integrase
    sacB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PEM7 and unknown

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-mGold-Calnexin-N-14

    Plasmid

    #158004

    Purpose

    Visualization of ER in mammalian cells using mGold

    Depositor

    Francois St-Pierre

    Article

    Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

    Insert

    Calnexin (CANX Human)
    

    Use

    Tags

    mGold

    Expression

    Mammalian

    Mutation

    I474N

    Promoter

    CMV

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. V406 4HA-CKIe(K38R)

    Plasmid

    #13725

    Depositor

    David Virshup

    Article

    Rivers et al J Biol Chem. 1998 Jun 26. 273(26):15980-

    Insert

    casein kinase 1 epsilon (CSNK1E Human)
    

    Use

    Tags

    4 HA

    Expression

    Mammalian

    Mutation

    kinase-inactive due to K38R mutation

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-SaCas9WT-2xNLS

    Plasmid

    #107320

    Purpose

    Expresses SpCas9 fused to SaCas9 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SpCas9-SaCas9 fusion
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    SpCas9 is fused to SaCas9

    Promoter

    CMV IE94

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP-GIT1

    Plasmid

    #15226

    Depositor

    Rick Horwitz

    Article

    Manabe et al J Cell Sci. 2002 Apr 1. 115(Pt 7):1497-5

    Insert

    G protein coupled receptor kinase-interacting protein 1 (GIT1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    L425V, E476K and L583P

    Promoter

    Available since

    Sept. 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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