We narrowed to 6,999 results for: nsf

1. EGFR (del3) L747-E749del, A750P

   Plasmid

   #11015

   Purpose

   Retroviral construct for expressing human EGFR with L747-E749 deletion and A750P mutation in human cells

   Depositor

   Matthew Meyerson

   Article

   Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

   Insert

   EGFR (del3) (EGFR Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   L747-E749 deleted, A750P

   Promoter

   Available since

   Dec. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

2. KAT14-pDEST10

   Plasmid

   #118382

   Purpose

   HIS-tagged insect cell expression vector with KAT14.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Insert

   KAT14 (KAT14 Human)
   

   Use

   Tags

   HIS

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   May 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5FRT/Flag-FLJ10081

   Plasmid

   #64775

   Purpose

   Mammalian expression of Flag-tagged FLJ10081 (NSL3)

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

   Insert

   FLJ10081 (KANSL3 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBabe H-Ras 12V

   Plasmid

   #12545

   Depositor

   Channing Der

   Article

   Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

   Insert

   H-Ras 12V (HRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   12V constituitively activated.

   Promoter

   Available since

   Sept. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pmCherry-N1-GalT

   Plasmid

   #87327

   Purpose

   To express GalT-mCherry in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferase

   Depositor

   Lei Lu

   Article

   Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15

   Insert

   1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGal-Trans-Myc-eGFP-KDEL

   Plasmid

   #177729

   Purpose

   ER protein collagen beta (1-O) galactosyltransferase fused to eGFP at the C-terminus, myc tagged

   Depositor

   Erik Böttger

   Article

   Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

   Insert

   collagen beta(1-O)galactosyltransferase 1 (COLGALT1 Human)
   

   Use

   Tags

   KDEL, Myc, and eGFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. kiCAP-AAV-BI-hTFR1-2

   Plasmid

   #218797

   Purpose

   RepCap for AAV production

   Depositor

   Ben Deverman

   Article

   Huang et al Science. 2024 Jun 14;384(6701):1220-1227

   Insert

   AAV Rep-Cap BI-hTFR1-2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. piRFP670-N1-GalT

   Plasmid

   #87325

   Purpose

   To express GalT-iRFP670 in mammalian cells, GalT encodes the 1-81 amino acids from β1,4-galactosyltransferase

   Depositor

   Lei Lu

   Article

   Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15

   Insert

   1-81 amino acids from β1,4-galactosyltransferase (B4GALT1 Human)
   

   Use

   Tags

   iRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19-U6-BsmBI_cassette-Nme/Nme2Cas9-sgRNA (KAC32)

   Plasmid

   #133794

   Purpose

   U6 promoter sgRNA entry vector used for all NmeCas9 or Nme2Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Amrani et al. Genome Biology 2018

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   NmeCas9 and Nme2Cas9 sgRNA entry vector
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   sgRNA architecture from Amrani et al. Genome Biol…

   Promoter

   U6

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-GRET

    Plasmid

    #158222

    Purpose

    Control lentiviral plasmid encoding GFP-Nluc BRET reporter from Schaub et al., Cancer Research, 2015.

    Depositor

    Charles P. Lai

    Article

    Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014

    Insert

    GFP-Nluc
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBabe N-Ras 61K

    Plasmid

    #12543

    Depositor

    Channing Der

    Article

    Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

    Insert

    NRas 61K (NRAS Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    61K constitutively activated.

    Promoter

    Available since

    Sept. 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. EYFP-p65

    Plasmid

    #111192

    Purpose

    fluorescent fusion protein

    Depositor

    Johannes A. Schmid

    Article

    Schmid et al J Biol Chem. 2000 Jun 2;275(22):17035-42

    Insert

    RELA (RELA Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pD40-His/V5-c-MycT58A

    Plasmid

    #45598

    Depositor

    Rosalie Sears

    Article

    Yeh et al Nat Cell Biol. 2004 Apr;6(4):308-18. Epu

    Insert

    c-Myc T58A (Myc Mouse)
    

    Use

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    T58A

    Promoter

    CMV

    Available since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

14. FLAG-PRMT6(V86K/D88A)

    Plasmid

    #164698

    Purpose

    Expression of PRMT6(V86K/D88A) with an N-terminal FLAG tag

    Depositor

    Cheryl Arrowsmith

    Article

    Eram et al ACS Chem Biol. 2016 Mar 18;11(3):772-781

    Insert

    PRMT6 (PRMT6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    V86K, D88A

    Promoter

    CMV

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAd-Track Flag HA PGC-1 alpha

    Plasmid

    #14426

    Depositor

    Pere Puigserver

    Article

    Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

    Insert

    PGC-1 alpha (Ppargc1a Mouse)
    

    Use

    Adenoviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Last four amino acids of PGC-1a removed to make t…

    Promoter

    Available since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only

16. TFRC-His-bio

    Plasmid

    #52338

    Purpose

    Expresses full-length Transferrin receptor protein 1 ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TFRC (TFRC Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDH-EF1-TfR20-SNAP-IRES-Puro

    Plasmid

    #171018

    Purpose

    Lentiviral expression of a transferrin receptor-SNAP-tag fusion in mammalian cells

    Depositor

    Angus Johnston

    Article

    FitzGerald et al Nat Commun. 2020 Sep 8;11(1):4482. doi:

    Insert

    Transferrin receptor (Human) - SNAP-tag (TFRC Human)
    

    Use

    Lentiviral

    Tags

    SNAPtag and TfR

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV5 HA-TBRII (delta Cyt)

    Plasmid

    #14051

    Depositor

    Joan Massague

    Article

    Siegel et al Proc Natl Acad Sci U S A. 2003 Jul 8. 10

    Insert

    TGF beta rec II (delta cyt) (TGFBR2 Human)
    

    Use

    Tags

    HA (PYDVPDYA)

    Expression

    Mammalian

    Mutation

    Dominant negative mutant. A stop codon was introd…

    Promoter

    Available since

    May 10, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. EGFP-SopB

    Plasmid

    #183657

    Purpose

    N-terminally tagged Salmonella Typhimurium SopB for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLVX-hMIGA2-DsRed

    Plasmid

    #192866

    Purpose

    Express codon-optimized human MIGA2 full length with a C-terminal DsRed tag in mammalian cells

    Depositor

    Karin Reinisch

    Article

    Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

    Insert

    Codon-optimized human Mitoguardin 2 (MIGA2 Human)
    

    Use

    Tags

    DsRed

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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