We narrowed to 32,558 results for: Eng

1. pYPQ166-iSpyMac

   Plasmid

   #149553

   Purpose

   Gateway entry clone for CRISPR-iSpyMacCas9 genome editing at NAAR PAMs

   Depositor

   Yiping Qi

   Article

   Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

   Insert

   iSpyMacCas9
   

   Use

   CRISPR; Gateway compatible zispymaccas9 entry clo…

   Tags

   3X FLAG, NLS and NLS

   Expression

   Plant

   Mutation

   R221K, N394K, Protospacer interacting domain form…

   Available Since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSET-pr-mEos3.1 (mEosFP3.1-A69T)

   Plasmid

   #99221

   Purpose

   Bacterial expression of the primed conversion capable protein pr-mEos3.1 (mEos3.1-A69T).

   Depositor

   Periklis Pantazis

   Article

   Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

   Insert

   pr-mEos3.1
   

   Expression

   Bacterial

   Mutation

   A69T for primed conversion applications

   Promoter

   T7

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-15b-LanCL1

   Plasmid

   #154189

   Purpose

   To express LanCL1 in bacterial cells

   Depositor

   Wilfred van der Donk

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   LanCL1
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. NG0750 pCI-TPI-PTC48-4H

   Plasmid

   #65803

   Purpose

   NMD reporter mRNA

   Depositor

   Niels Gehring

   Article

   Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

   Insert

   TPI (TPI1 Human)
   

   Tags

   4H (4 copies of short beta-globin 3'UTR for …

   Expression

   Mammalian

   Mutation

   premature stop codon at 48

   Promoter

   CMV

   Available Since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETDuet1-WT-LanCL2-C-Ter-His

   Plasmid

   #154186

   Purpose

   To express LanCL2 in bacterial cells

   Depositor

   Wilfred van der Donk

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   LanCL2
   

   Tags

   Hexahistidine

   Expression

   Bacterial

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDIRECT_21B

   Plasmid

   #91129

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9_dead + AtU6:gRNA, Plant Selection: 2x35S:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: 35S:AtCas9_dead + AtU6:gRNA
   

   Use

   CRISPR

   Expression

   Plant

   Mutation

   D10A, H840A

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHAGE2-TetO-GFP-mSox2

   Plasmid

   #206374

   Purpose

   Expresses EGFP fused mouse SOX2 in mammalian cells, for lentivirus generation.

   Depositor

   Ralf Jauch

   Article

   Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

   Insert

   Sox2 (Sox2 Mouse)
   

   Use

   Lentiviral

   Tags

   EGFP

   Available Since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWPXL-p150

   Plasmid

   #160992

   Purpose

   Express fulllength of mouse p150 protein

   Depositor

   Zhiguo Zhang

   Article

   Cheng et al Nucleic Acids Res. 2019 Dec 2;47(21):111

   Insert

   CHAF1A (Chaf1a Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSET-pr-mEos4b (mEosFP4b-V69T)

   Plasmid

   #99226

   Purpose

   Bacterial expression of the primed conversion capable protein pr-mEos4b (mEos4b-V69T).

   Depositor

   Periklis Pantazis

   Article

   Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

   Insert

   pr-mEos4b
   

   Expression

   Bacterial

   Mutation

   V69T for primed conversion applications

   Promoter

   T7

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBTK614

    Plasmid

    #110608

    Purpose

    BTK assembled plasmid - Encodes dCas9 with no targeting sgRNA (used in Stage 2 assembly to repress target genes)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    dCas9
    

    Use

    Synthetic Biology

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDIRECT_23D

    Plasmid

    #91141

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A, H840A

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBTK601

    Plasmid

    #110607

    Purpose

    BTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration)

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    Cas9
    

    Use

    Synthetic Biology

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYPQ292 (AaCas12b)-D570A-TV-MS2-TV

    Plasmid

    #136381

    Purpose

    CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-TV fusion protein

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b (D570A)-TV-T2A-MCP-TV
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D570A

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDIRECT_25G

    Plasmid

    #91144

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A, H840A

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDIRECT_25I

    Plasmid

    #91146

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A, H840A

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTK-EdnrbE2-Sox10-FLAG-tev-AVI

    Plasmid

    #127775

    Purpose

    Modified pTK plasmid containing chicken EdnrB E2 enhancer driving expression of full length Chicken Sox10 in frame with a Flag-tag and Avi-tag

    Depositor

    Tatjana Sauka-Spengler

    Article

    Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

    Insert

    sox10 (SOX10 Chicken)
    

    Use

    Enhancer reporter construct

    Tags

    Avi

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDIRECT_22D

    Plasmid

    #91136

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:npt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A, H840A

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNTPTP alpha (S180A/S204A)

    Plasmid

    #17700

    Depositor

    Xinmin Zheng

    Article

    Zheng et al J Biol Chem. 2002 Jun 14. 277(24):21922-

    Insert

    PTPalpha S180A S204A (PTPRA Human)
    

    Expression

    Mammalian

    Mutation

    S180A / S204A

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pDIRECT_21D

    Plasmid

    #91131

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A, H840A

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NG1237 pCI-TPI-PTC160-4H

    Plasmid

    #65804

    Purpose

    NMD reporter mRNA

    Depositor

    Niels Gehring

    Article

    Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

    Insert

    TPI (TPI1 Human)
    

    Tags

    4H (4 copies of short beta-globin 3'UTR for …

    Expression

    Mammalian

    Mutation

    premature stop codon at 160

    Promoter

    CMV

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge