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We narrowed to 3,128 results for: try

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Showing: 2881 - 2900 of 3128 results

  1. pLuc-OptoJNKi-lsm

    Plasmid
    #89746
    Purpose
    Expression of light-regulated JNK inhibitor, firefly luciferase-fused, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11
    Depositor
    Michael Courtney
    Publication
    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10
    Insert
    OptoJNKi lit-state mutant
    Use
    Luciferase
    Tags
    Firefly luciferase
    Expression
    Mammalian
    Mutation
    I539E mutation of AsLov2Jalpha
    Promoter
    CMV
    Available since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    89746-plasmid-map-sequence-id-192490

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  2. pLuc-OptoJNKi-dsm

    Plasmid
    #89745
    Purpose
    Expression of light-regulated JNK inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11
    Depositor
    Michael Courtney
    Publication
    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10
    Insert
    OptoJNKi dark-state mutant
    Use
    Luciferase
    Tags
    Firefly luciferase
    Expression
    Mammalian
    Mutation
    C450A mutation of AsLov2Jalpha
    Promoter
    CMV
    Available since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    89745-plasmid-map-sequence-id-192487

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  3. mutpRL-TK 1x perfect to bulged siRNA

    Plasmid
    #40765
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Publication
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    CXCR4 small RNA perfect to bulged target site
    Use
    Luciferase
    Tags
    luciferase
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available since
    Dec. 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  4. mutpRL-TK 1x perfect to seed siRNA

    Plasmid
    #40766
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Publication
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    CXCR4 small RNA perfect to seed target site
    Use
    Luciferase
    Tags
    luciferase
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  8. pET28a HaloTag-TEVc-6xtrTUBE

    Plasmid
    #254153
    Purpose
    Bacterial expression of 6xtrTUBE with an N-terminal HaloTag followed by a TEV protease cleavage site
    Depositor
    Rahul Samant
    Publication
    Johnston et al J. Proteome Res. 2026
    Insert
    6xtrTUBE (UBQLN1 Human, Synthetic)
    Tags
    HaloTag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    All Arg residues in the UBA domain are mutated to…
    Promoter
    T7
    Available since
    Aug. 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    254153-plasmid-map-sequence-id-511309

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  9. pcDNA6-myc-BKαΔM-GFP-V5-His

    Plasmid
    #255910
    Purpose
    Complementary plasmid for co-expression with BKαΔM module to form functional BK channels. BKα residues 94–651 are replaced by a flexible peptide linker and GFP is tagged at C-terminus.
    Depositor
    Jiusheng Yan
    Publication
    Chen et al Elife. 2026 Mar 5;14:RP107681. doi: 10.7
    Insert
    KCNMA1 (partial) (KCNMA1 Human)
    Tags
    EGFP-V5-6xHis and Myc
    Expression
    Mammalian
    Mutation
    residues 94–651 are deleted and replaced by a fle…
    Available since
    June 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    255910-plasmid-map-sequence-id-512404

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  10. pLenti gRNA KO OCLNx2 spCas9-T2A-Crimson-P2A-puro

    Plasmid
    #208398
    Purpose
    codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, E2-Crimson fluorescent protein and puromycin resistance
    Depositor
    Raphael Gaudin
    Publication
    Pradel et al Autophagy. 2024 Aug;20(8):1825-1836. doi
    Insert
    hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
    Use
    CRISPR and Lentiviral
    Tags
    E2-Crimson
    Expression
    Mammalian
    Available since
    Dec. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    208398-plasmid-map-sequence-id-417651

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  12. pLuc-Optop38i-dsm

    Plasmid
    #89748
    Purpose
    Expression of light-regulated p38 inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor MK3BD3-13F
    Depositor
    Michael Courtney
    Publication
    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10
    Insert
    Optop38i dark-state mutant
    Use
    Luciferase
    Tags
    Firefly luciferase
    Expression
    Mammalian
    Mutation
    C450A mutation of AsLov2Jalpha
    Promoter
    CMV
    Available since
    Aug. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    89748-plasmid-map-sequence-id-192493

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  13. pcDNA3.1-nAChr-Alpha6-CFP

    Plasmid
    #50482
    Purpose
    this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF
    Depositor
    Henry Lester
    Publication
    Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu
    Insert
    nAChr-alpha6 (Chrna6 Mouse)
    Tags
    CFP fusion in M3-M4 loop (after residue A405)
    Expression
    Mammalian
    Mutation
    Gly-Ala-Gly flexible linker flanking the CFP open…
    Promoter
    CMV
    Available since
    Jan. 13, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. mutpRL-TK 1x perfect to seed plus 13-16 siRNA

    Plasmid
    #40767
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Phillip Zamore
    Publication
    Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011
    Insert
    CXCR4 small RNA perfect to seed plus 13-16 target site
    Use
    Luciferase
    Tags
    luciferase
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only

  19. pET21a(+)-Thio6-His6-TEV-TARDBP(Δ1-273, W385tag)

    Plasmid
    #260639
    Purpose
    Expresses TDP-43(Δ1-273, W385tag) for Amber codon suppression in bacterial strains
    Depositor
    Jennifer Lee
    Publication
    Watson et al J Am Chem Soc. 2026 Jun 17;148(23):23494
    Insert
    Transactive response DNA binding protein 43 (TARDBP Human)
    Tags
    thio6 expression sequence, hexahistidine, TEV pro…
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-273, mutated Tryptophan 385…
    Promoter
    T7
    Available since
    Sept. 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    260639-plasmid-map-sequence-id-521733

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  20. pET28a 6xHis-HaloTag-TEVc-4xtrTUBE

    Plasmid
    #254154
    Purpose
    Bacterial expression of 4xtrTUBE with an N-terminal 6xHis and HaloTag followed by a TEV protease cleavage site
    Depositor
    Rahul Samant
    Publication
    Johnston et al J. Proteome Res. 2026
    Insert
    4xtrTUBE (UBQLN1 Human, Synthetic)
    Tags
    HaloTag, His6, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    All Arg residues in the UBA domain are mutated to…
    Promoter
    T7
    Available since
    Aug. 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    254154-plasmid-map-sequence-id-511316

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Showing: 2881 - 2900 of 3128 results