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pHAGE-PIK3CA-K111delK

Plasmid

#116505

Purpose

Lentiviral expression of PIK3CA K111delK

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

K111delK

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3CA_p.E726K

Plasmid

#82845

Purpose

Gateway Donor vector containing PIK3CA, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PIK3CA (PIK3CA Human)

Use

Gateway Cloning

Mutation

E726K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Cherry-SRBC

Plasmid

#69492

Purpose

Expression of fluorescently tagged SRBC in mammalian cells

Depositor

Rob Parton

Article

Gambin et al Elife. 2013 Jan 1;3:e01434. doi: 10.7554

Insert

SRBC (Cavin3 Mouse)

Tags

Cherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2016

Availability

Academic Institutions and Nonprofits only
mPapaya1-Actin-C-18

Plasmid

#56400

Purpose

Localization: Actin, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

Actin (ACTB Human)

Tags

mPapaya1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
pET41a-MED19

Plasmid

#15430

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED19 (Med19 Mouse)

Tags

GST and His

Expression

Bacterial

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pET41a-MED30

Plasmid

#15431

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED30 (Med30 Mouse)

Tags

GST and His

Expression

Bacterial

Mutation

N/A

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
pUb FLAG-tws

Plasmid

#195068

Purpose

Expresses FLAG-tagged Drosophila tws protein

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

twins (tws Fly)

Tags

3xFLAG

Expression

Insect

Promoter

Ubi-p63e

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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Sema3c(S)-AP-His

Plasmid

#72016

Purpose

Expresses the Sema3C protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3c (Sema3c Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2016

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Actin-C-18

Plasmid

#55400

Purpose

Localization: Actin, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Actin (ACTB Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
DPP7 (3N0T)

Plasmid

#28114

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

DPP7:SDC134-D09:C41107 (DPP7 Human)

Tags

His

Expression

Bacterial

Mutation

contains amino acid residues 28-492

Available Since

March 18, 2011

Availability

Industry, Academic Institutions, and Nonprofits
pGEX-KG-MmUbc7/2783

Plasmid

#11490

Depositor

Allan Weissman

Article

Tiwari et al J Biol Chem. 2001 May 11. 276(19):16193-

Insert

MmUbc7 (Ube2g2 Mouse)

Tags

GST

Expression

Bacterial

Mutation

n/a

Available Since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

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pFastBac Mel18 flag

Plasmid

#1969

Depositor

Robert Kingston

Article

Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

Insert

Mel18 (Pcgf2 Mouse)

Tags

flag

Expression

Insect

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
pT7-MLH1dn (mouse) for IVT

Plasmid

#246485

Purpose

Template for in vitro transcription of mouse MLH1dn

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

mouse MLH1dn (Mlh1 Mouse)

Use

Template for in vitro transcription

Mutation

deletion of residues 758-760

Promoter

T7

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_CUX1_NUTM1

Plasmid

#205800

Purpose

Express mEGFP-tagged fusion protein, CUX1_NUTM1 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

CUX1_NUTM1 (CUX1, NUTM1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pMM7

Plasmid

#201213

Purpose

Integrative GFP-based shuttle vector for genetic engineering of Parageobacillus thermoglucosidasius. Carries homologous flanks for deletion of key sporulation regulator spo0A.

Depositor

Alex Nielsen

Article

Millgaard et al AMB Express. 2023 May 8;13(1):44. doi: 1

Inserts

sfGFP
Spo0A right flank
Spo0A left flank

Use

Synthetic Biology

Expression

Bacterial

Mutation

F11V, T13P, N39D, A179A

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pTS2362_Tier3(SB)-rtTA-PuroR

Plasmid

#169658

Purpose

Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-PuroR-pA-3'ITR)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven rtTA expression and PRPBSA-driven PuroR expression

Expression

Mammalian

Promoter

PRPBSA

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-AviMta3-3xFiBsd

Plasmid

#140962

Purpose

full-length wt Avi-Mta3-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cells

Depositor

Brian Hendrich

Article

Burgold et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

Insert

metastasis associated 3 (Mta3 Mouse, Synthetic)

Tags

3xFLAG, Avi, and TEV

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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CBP-Bromo-HAT-SP65

Plasmid

#21090

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Insert

CBP (Crebbp Mouse)

Use

In vitro tnt

Tags

Flag

Mutation

Bromo+HAT domains aa 1107-1621

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pShuttle-PR.CC9

Plasmid

#192932

Purpose

pShuttle.CC9 encoding sgRNAs targeting Trp53 and Rb1 genes

Depositor

Thorsten Stiewe

Article

Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

Insert

Trp53, Rb1 (Trp53, Rb1 Mouse)

Use

Gateway-compatible vector

Promoter

U6

Available Since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-SERCA1

Plasmid

#109498

Purpose

Muscle specific zebrafish expression of mKate2 fused to sarcoplasmic/endoplasmic calcium ATPase 1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

SERCA1 (atp2a1 Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Mutation

Please see Depositor Comments

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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