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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,228 results for: Streptococcus

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Showing: 2901 - 2920 of 3228 results

  3. pSCBE3-HF1

    Plasmid
    #218156
    Purpose
    This plasmid harbors the base editor SCBE3-HF1 along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing in Streptomyces.
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    scbe3-HF1
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutations in the portion of SpCas9 : D10A / N497A…
    Promoter
    rpsL promoter
    Available Since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    218156-plasmid-map-sequence-id-434901

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  4. pAAV-CMV-dSa VP64 Hbb

    Plasmid
    #99693
    Purpose
    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Hbb promoter, vector allows for activation of mouse Hbb, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Hbb (gRNA: GGGGTAAGGGGAGCAAGGTC) (Hbb Mouse, Synthetic)
    Use
    AAV and CRISPR
    Tags
    VP64
    Mutation
    dead Cas9
    Available Since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    99693-plasmid-map-sequence-id-194561

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  5. dSp-Rta 125-175

    Plasmid
    #99668
    Purpose
    Expresses dSp Cas9 fused to truncated RTA activation domain
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9
    Tags
    truncated RTA activation domain (125-175)
    Expression
    Mammalian
    Mutation
    dead Cas9
    Available Since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    99668-plasmid-map-sequence-id-189832

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  7. dSp-p65 (1-100)

    Plasmid
    #99660
    Purpose
    Expresses dSp Cas9 fused to truncated p65 activation domain
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9
    Tags
    truncated p65 activation domain (1-100)
    Expression
    Mammalian
    Mutation
    dead Cas9
    Available Since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    99660-plasmid-map-sequence-id-189821

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  8. dSp-p65 (50-150)

    Plasmid
    #99661
    Purpose
    Expresses dSp Cas9 fused to truncated p65 activation domain
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9
    Tags
    truncated p65 activation domain (50-150)
    Expression
    Mammalian
    Mutation
    dead Cas9
    Available Since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    99661-plasmid-map-sequence-id-189830

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  10. dSp-Rta 75-190

    Plasmid
    #99663
    Purpose
    Expresses dSp Cas9 fused to truncated RTA activation domain
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9
    Tags
    truncated RTA activation domain (75-190)
    Expression
    Mammalian
    Mutation
    dead Cas9
    Available Since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    99663-plasmid-map-sequence-id-192026

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  11. dSp-Rta 75-175

    Plasmid
    #99666
    Purpose
    Expresses dSp Cas9 fused to truncated RTA activation domain
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9
    Tags
    truncated RTA activation domain (75-175)
    Expression
    Mammalian
    Mutation
    dead Cas9
    Available Since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    99666-plasmid-map-sequence-id-192042

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  13. dSp-p65 (150-261)

    Plasmid
    #99655
    Purpose
    Expresses dSp Cas9 fused to truncated p65 activation domain
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9
    Tags
    truncated p65 activation domain (150-261)
    Expression
    Mammalian
    Mutation
    dead Cas9
    Available Since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    99655-plasmid-map-sequence-id-191918

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  14. dSp-p65 (100-261)

    Plasmid
    #99656
    Purpose
    Expresses dSp Cas9 fused to truncated p65 activation domain
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9
    Tags
    truncated p65 activation domain (100-261)
    Expression
    Mammalian
    Mutation
    dead Cas9
    Available Since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    99656-plasmid-map-sequence-id-191924

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  15. dSp-p65 (200-261)

    Plasmid
    #99657
    Purpose
    Expresses dSp Cas9 fused to truncated p65 activation domain
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9
    Tags
    truncated p65 activation domain (200-261)
    Expression
    Mammalian
    Mutation
    dead Cas9
    Available Since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    99657-plasmid-map-sequence-id-191931

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  16. dSp-p65 (1-200)

    Plasmid
    #99658
    Purpose
    Expresses dSp Cas9 fused to truncated p65 activation domain
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9
    Tags
    truncated p65 activation domain (1-200)
    Expression
    Mammalian
    Mutation
    dead Cas9
    Available Since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    99658-plasmid-map-sequence-id-191936

    Enlarge

  17. dSp-p65 (1-150)

    Plasmid
    #99659
    Purpose
    Expresses dSp Cas9 fused to truncated p65 activation domain
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9
    Tags
    truncated p65 activation domain (1-150)
    Expression
    Mammalian
    Mutation
    dead Cas9
    Available Since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    99659-plasmid-map-sequence-id-191939

    Enlarge

  18. pSCBE3-NG-HF1

    Plasmid
    #218160
    Purpose
    This plasmid harbors the base editor SCBE3-NG-HF1 along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    scbe3-NG-HF1
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutations in the portion of SpCas9 : D10A / N497A…
    Promoter
    rpsL promoter
    Available Since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218160-plasmid-map-sequence-id-433202

    Enlarge

  19. pSCBE3-NG-Hypa

    Plasmid
    #218161
    Purpose
    This plasmid harbors the base editor SCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    scbe3-NG-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutations in the portion of SpCas9 : D10A / N692A…
    Promoter
    rpsL promoter
    Available Since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218161-plasmid-map-sequence-id-433271

    Enlarge

  20. pSCBE3-NG-HF1-Hypa

    Plasmid
    #218162
    Purpose
    This plasmid harbors the base editor SCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    scbe3-NG-HF1-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutations in the portion of SpCas9 : D10A / N497A…
    Promoter
    rpsL promoter
    Available Since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    218162-plasmid-map-sequence-id-433200

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Showing: 2901 - 2920 of 3228 results