We narrowed to 6,999 results for: nsf

1. SIN-cPPT-H1-sgHTT1-U6-sgGFP2-7SK-sgCas9-PGK-mCherry-WPRE

   Plasmid

   #87919

   Purpose

   HIV-1 SIN lentiviral transfer vector

   Depositor

   Nicole Déglon

   Article

   Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

   Insert

   sgHTT1, shGFP2, sgCas9, cherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   H1, U6, 7SK, PGK

   Available since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28-MRPP3-His

   Plasmid

   #67866

   Purpose

   bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. His tag

   Depositor

   Walter Rossmanith

   Article

   Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583

   Insert

   MRPP3 (KIAA0391 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Amino acid 46 of recombinant MRPP3 is preceded by…

   Promoter

   T7

   Available since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXs-hu-c-Myc-dN2

   Plasmid

   #50774

   Purpose

   retroviral expression of human c-Myc dN2 fragment

   Depositor

   Shinya Yamanaka

   Article

   Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

   Insert

   c-Myc -dN2 (MYC Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   N-terminal deletion, contains aa 42-439

   Promoter

   Available since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(-)-V5.hOr47a

   Plasmid

   #126474

   Purpose

   Human codon-optimized version of D. melanogaster Or47a (hOr47a) without N-terminal tags for improved intracellular trafficking in mammalian cells

   Depositor

   Bill Hansson

   Article

   Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

   Insert

   Or47a (Or47a Fly)
   

   Use

   Tags

   V5 tag

   Expression

   Mammalian

   Mutation

   codon optimization for H. sapiens

   Promoter

   CMV

   Available since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMXs-hu-c-Myc-dN1

   Plasmid

   #50773

   Purpose

   retroviral expression of human c-Myc dN1 fragment

   Depositor

   Shinya Yamanaka

   Article

   Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

   Insert

   c-Myc -dN1 (MYC Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   N-terminal deletion, contains aa 14-439

   Promoter

   Available since

   Feb. 28, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pS6-C-hα2M-H6

   Plasmid

   #128497

   Purpose

   Expresses human C-α2M (from F789 to S1468) in mammalian cells. With C-t H6x tag.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

   Insert

   A2M (F789-S1468) (A2M Human)
   

   Use

   Tags

   His tag (6x)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCI-neo-FHM

   Plasmid

   #51871

   Purpose

   Expresses N-terminal FLAG-HA-tagged full length rat MIBP1 (cloned into pCI-neo)

   Depositor

   Tomoko Tahira, Kenshi Hayashi

   Article

   Iwashita et al J Biol Chem. 2012 Mar 23;287(13):9887-90

   Insert

   MIBP1 (Hivep2 Rat)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pUC19-U6-BsmBI_cassette-CjeCas9-sgRNA (KAC482)

   Plasmid

   #133793

   Purpose

   U6 promoter sgRNA entry vector used for all CjeCas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V2-TGAA linker sgRNA architecture from Kim et al. Nature Communications 2017

   Depositor

   Benjamin Kleinstiver, Joseph Bondy-Denomy

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   CjeCas9 sgRNA entry vector
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   V2-TGAA linker sgRNA architecture from Kim et al.…

   Promoter

   U6

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. His6-SMT3-SopB

   Plasmid

   #183676

   Purpose

   Recombinant Salmonella Typhimurium SopB

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   sopB (sopB Budding Yeast, Salmonella enterica serovar Typhimurium)
   

   Use

   Tags

   His6-S. cerevisiae SMT3(2-101)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGK-hMIGA2-DsRed

    Plasmid

    #192867

    Purpose

    Express codon-optimized human MIGA2 full length with a C-terminal DsRed tag in mammalian cells

    Depositor

    Karin Reinisch

    Article

    Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

    Insert

    Codon-optimized human Mitoguardin 2 (MIGA2 Human)
    

    Use

    Tags

    DsRed

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. gRNAs[bTub+Sxl].1046B

    Plasmid

    #112692

    Purpose

    express two gRNA targeting bTub & Sxl under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    U6.3-gRNA[bTub] and U6.3-gRNA[Sxl] (Sxl Fly)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST47-ARF4-GFP

    Plasmid

    #67392

    Purpose

    Mammalian expression of hArf4 with C-term GFP tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARF4 (ARF4 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    V53A, L123P and M134I mutations were unintended P…

    Promoter

    CMV

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pLKO.TRC1.shmNsd1.2, puro

    Plasmid

    #114447

    Purpose

    Lentiviral vector for expression of shRNA sequence targeting mouse Nsd1

    Depositor

    Adrian Bracken

    Article

    Streubel et al Mol Cell. 2018 Apr 19;70(2):371-379.e5.

    Insert

    shNsd1.2 (Nsd1 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.TRC1.shmNsd1.1, puro

    Plasmid

    #114446

    Purpose

    Lentiviral vector for expression of shRNA sequence targeting mouse Nsd1

    Depositor

    Adrian Bracken

    Article

    Streubel et al Mol Cell. 2018 Apr 19;70(2):371-379.e5.

    Insert

    shNsd1.1 (Nsd1 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJHG004

    Plasmid

    #126455

    Purpose

    Expresses dTdT in mammalian cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    dTdT (DNTT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Added D343E and D345E to inactivate the enzyme.

    Promoter

    CMV

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRC/RSV-rat Agt

    Plasmid

    #122104

    Purpose

    Expression of angiotensinogen

    Depositor

    John Chan

    Article

    Zhang et al J Am Soc Nephrol. 2002 Feb;13(2):302-12.

    Insert

    angiotensinogen (Agt Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RSV promoter

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBullet-cg-c

    Plasmid

    #53070

    Purpose

    destination vector with cis-golgi marker (alpha-man I 49AA) -ECFP for tagged fluorescent protein (C-ter-EYFP) of plant gene

    Depositor

    Joshua Heazlewood

    Article

    Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV5-Flag-EPLIN beta delta LIM domain

    Plasmid

    #40938

    Depositor

    Scott Maul, Elizabeth Luna

    Article

    Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

    Insert

    EPLIN beta delta LIM domain (LIMA1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    LIM domain deleted

    Promoter

    Available since

    Dec. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pBullet-vac-c

    Plasmid

    #53076

    Purpose

    destination vector with vacuole marker (gamma-TIP) - ECFP for tagged fluorescent protein (C-ter-EYFP) of plant gene

    Depositor

    Joshua Heazlewood

    Article

    Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBullet-er-c

    Plasmid

    #53068

    Purpose

    destination vector with WAK2 (29 aa)-ECFP- ER marker (HDEL) for tagged fluorescent protein (C-ter-EYFP) of plant gene

    Depositor

    Joshua Heazlewood

    Article

    Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    ECFP and EYFP

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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