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pET22b-ecDHFR-dCys

Plasmid

#110541

Purpose

Bacterial protein of E.coli DHFR C85A/C152S mutant

Depositor

Stephen Benkovic

Article

Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

Insert

ecDHFR

Expression

Bacterial

Mutation

C85 mutated to A85, C152 mutated to S152

Promoter

T7

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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MT583

Plasmid

#63114

Purpose

The gDNA construct is designed for arabidopsis ecotype Est-1 ACD6 expression in other genetic backgrounds.

Depositor

Detlef Weigel

Article

Todesco et al Nature. 2010 Jun 3;465(7298):632-6. doi:

Insert

ACD6 (Accelerated Cell Death 6)

Expression

Plant

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only

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MT79

Plasmid

#63112

Purpose

The artificial microRNA is designed for down-regulating expression of ACD6 mRNA.

Depositor

Detlef Weigel

Article

Todesco et al Nature. 2010 Jun 3;465(7298):632-6. doi:

Insert

amiR-ACD6

Expression

Plant

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pET-20b-T7-Ntx

Plasmid

#58714

Purpose

Expresses T7-Notexin in Escherichia coli

Depositor

Fiorella Tonello

Article

Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

Insert

Notexin

Tags

T7 peptide tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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refGFP1_pQE30

Plasmid

#48644

Purpose

Bact exp vector encoding for the 6XHis tagged insensitive variant of the fluorescent redox probe roGFP with mut Cys147 to Serine, del insertion147b and mutated Cys204 to Glut.Referred to as refGFP

Depositor

David Ron

Article

Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

Insert

refGFP1

Tags

6xHis

Expression

Bacterial

Mutation

refGFP C147S, C204Q

Available Since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-PKCsub-RLuc8N-NES

Plasmid

#138213

Purpose

Encodes substrate fragment of luminescence-based bimolecular PKC activity reporter (LumCKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.

Depositor

Jin Zhang

Article

Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

Insert

mCherry-PKCsub-RLuc8N-NES

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Twin-Strep_IRES-mRuby2

Plasmid

#113885

Purpose

Mammalian (inducible) protein expression, bicistronic expression of cytosolic mRuby2

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Promoter

CMV-MIE-TetO2

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Twin-Strep_IRES-mTurquoise2

Plasmid

#113886

Purpose

Mammalian (inducible) protein expression, bicistronic expression of cytosolic mTurquoise2

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Twin-Strep

Promoter

CMV-MIE-TetO2

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Avi-His6_IRES-mRuby2

Plasmid

#113889

Purpose

Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mRuby2

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Avi-His6

Promoter

CMV-MIE-TetO2

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-mVenus-Twin-Strep

Plasmid

#113891

Purpose

Mammalian (inducible) protein expression, C-terminal 3C-mVenus-Twin-Strep fusion

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-mVenus-Twin-Strep

Promoter

CMV-MIE-TetO2

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Avi-His6_IRES-mTurquoise2

Plasmid

#113890

Purpose

Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic mTurquoise2

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Avi-His6

Promoter

CMV-MIE-TetO2

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pET30a-GABAT

Plasmid

#239572

Purpose

Expresses E. coli GABA transaminase (GABA-T/gabT) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography.

Depositor

William Plaxton

Article

Benidickson et al Biol Methods Protoc. 2025 Jun 20;10(1):b

Insert

4-aminobutyrate aminotransferase (gabT E. coli)

Tags

His6

Expression

Bacterial

Available Since

July 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET30a-SSDH

Plasmid

#239573

Purpose

Expresses E. coli succinate semialdehyde dehydrogenase (SSDH/gabD) for recombinant protein purification. Contains a His6-tag on its C-terminus for Ni2+ affinity chromatography.

Depositor

William Plaxton

Article

Benidickson et al Biol Methods Protoc. 2025 Jun 20;10(1):b

Insert

Succinate-semialdehyde dehydrogenase (gabD E. coli)

Tags

His6

Expression

Bacterial

Available Since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-P3-IgKL-SpyCatcher003-mNeonGreen

Plasmid

#234543

Purpose

to express a green fluorescent protein tagged with SpyCatcher in the liver

Depositor

Hajime Hirase

Article

Vittani et al bioRxiv 2025.03.18.643859

Insert

IgKL-SpyCatcher003-mNeonGreen

Use

AAV

Mutation

None

Promoter

P3

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-iGlucoSnFR-TS

Plasmid

#127952

Purpose

Expresses the iGlucoSnFR-TS biosensor for intracellular [glucose], with a fluorescence-lifetime readout, in mammalian cells

Depositor

Gary Yellen

Article

Diaz-Garcia et al J Neurosci Res. 2019 May 20. doi: 10.100

Insert

iGlucoSnFR-TS

Use

AAV

Tags

His6 and Xpress epitope

Expression

Mammalian

Promoter

CAG

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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piRFP

Plasmid

#31857

Purpose

a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

Depositor

Vladislav Verkhusha

Article

Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

Has Service

Cloning Grade DNA

Insert

iRFP

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…

Promoter

CMV

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pLenti-FlaviA-GFP-puro

Plasmid

#140088

Purpose

Fluorescence activatable reporter of flavivirus NS2B-NS3 protease activity

Depositor

Jorge Luis Arias-Arias

Article

Arias-Arias et al J Biol Chem. 2020 Jan 9. pii: RA119.0113

Insert

FlaviA-GFP

Use

Lentiviral; Low expression

Tags

6XHis tag and GFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pC3.1-caggs-Crimson-CAAX

Plasmid

#190041

Purpose

Red Fluorescent Protein for Long-Term Labeling of Fine Structures in Neurons

Depositor

Michael Lin

Article

Ning et al Front Cell Dev Biol. 2022 Jun 29;10:8934

Insert

Crimson

Tags

CAAX

Expression

Mammalian

Promoter

caggs

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pUC-GFP-AT

Plasmid

#133306

Purpose

"Burdensome" GFP expressing plasmid carrying axe/txe toxin-antitoxin for plasmid stabilisation

Depositor

Chris Barnes

Article

Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

Insert

axe/txe toxin-antitoxin cassette

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-135

Plasmid

#118553

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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