We narrowed to 32,558 results for: Eng

1. pYPQ141-ZmUBI-RZ-Cas12j2

   Plasmid

   #189781

   Purpose

   Cas12j2 (CasΦ) Gateway gRNA entry plasmid using Zea mays Ubi promoter and HH, HDV ribozyme processing

   Depositor

   Yiping Qi

   Article

   Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

   Insert

   gRNA cloning site
   

   Use

   CRISPR

   Available Since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIPKAH

   Plasmid

   #185869

   Purpose

   Knocking out GPP1 and expressing a phosphoketolase pathway (Lactobacillus reuteri xfp and Methanosarcina barkeri pta)

   Depositor

   Claudia Vickers

   Article

   Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

   Insert

   GPP1(-220,80)> PAg.TEF1>hphMX6>TAg.TEF1-PENO2> Lr.xfp>T_TPI1-PTDH3>Mb.pta>TFBA1-GPP1(754, 1400)
   

   Expression

   Yeast

   Mutation

   None

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTK-EdnrbE2-AVI-Tfap2B-tev-FLAG-2A-Citrine

   Plasmid

   #127776

   Purpose

   Modified pTK plasmid containing chicken EdnrB E2 enhancer driving expression of full length Chicken Tfap2B in frame with an Avi tag at the N terminus and FLAG-tag and Citrine at the C-terminus

   Depositor

   Tatjana Sauka-Spengler

   Article

   Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

   Insert

   tfap2b (TFAP2B Chicken)
   

   Use

   Unspecified

   Tags

   Avi

   Available Since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. PatoK-OG241

   Plasmid

   #72840

   Purpose

   NOT induced by acetoacetate to express GFP. Contains the native atoDAEB promoter (Pato) from E. coli DH5A, but out-of-frame with GFP gene. OG241 backbone.

   Depositor

   Chris Barnes

   Article

   Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

   Insert

   PatoK
   

   Expression

   Bacterial

   Promoter

   PatoK

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. PatoL-LOG241

   Plasmid

   #72846

   Purpose

   Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). LOG241 backbone.

   Depositor

   Chris Barnes

   Article

   Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

   Insert

   PatoL
   

   Expression

   Bacterial

   Promoter

   PatoL

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. PatoK-LOG241

   Plasmid

   #72848

   Purpose

   NOT induced by acetoacetate to express GFP. Contains the native atoDAEB promoter (Pato) from E. coli DH5A, but out-of-frame with GFP gene. LOG241 backbone.

   Depositor

   Chris Barnes

   Article

   Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

   Insert

   PatoK
   

   Expression

   Bacterial

   Promoter

   PatoK

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. Gal4pBS134-Pleum

   Plasmid

   #60339

   Purpose

   Contains a promoter of three gal binding sites derived from native galactose promoter coupled with a core promoter derived from native Leucine promoter.

   Depositor

   Hal Alper

   Article

   Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

   Insert

   promoter
   

   Use

   Synthetic Biology; Yeast synthetic hybrid promote…

   Expression

   Yeast

   Available Since

   Feb. 11, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pEXP5-NT-5th R

   Plasmid

   #42572

   Depositor

   Ivan Korendovych

   Article

   Moroz et al Angew Chem Int Ed Engl. 2013 Apr 29. doi

   Insert

   mutated calmodulin gene
   

   Tags

   Polyhistidine (6×His) region, TEV recognition sit…

   Expression

   Bacterial

   Mutation

   changed Phenylalanine 92 to Glutamine acid, Isole…

   Promoter

   T7

   Available Since

   March 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFBOH-LIC_DDB1:1-1140_delta:396-689

   Plasmid

   #210897

   Purpose

   Insect expression of full-length human DDB1 with a loop deletion I396 to D689. Untagged.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   DDB1:M1-H1140_delta:I396-D689 (DDB1 Human)
   

   Expression

   Insect

   Mutation

   loop deletion amino acids I396 to D689

   Promoter

   Polyhedrin

   Available Since

   March 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pFBOH-avi-TEV-LIC_DCAF12:1-453

    Plasmid

    #210940

    Purpose

    Insect expression of full-length human DCAF12. N-terminal 6X His tag, biotin acceptor peptide tag and TEV cleavage site.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    DCAF12:M1-S453 (DCAF12 Human)
    

    Expression

    Insect

    Mutation

    R131Q natural variant (VAR_035322)

    Promoter

    Polyhedrin

    Available Since

    March 20, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pAD100-CHIP-myc-His

    Plasmid

    #236054

    Purpose

    Mammalian expression of human CHIP with a C-terminal myc-His tag

    Depositor

    Jonathan Schisler

    Article

    Stewart et al bioRxiv 2025.02.17.637997

    Insert

    CHIP (STUB1 Human)
    

    Tags

    myc-His

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available Since

    Aug. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV_ENVLPE+

    Plasmid

    #232427

    Purpose

    Expression plasmid to produce ENVLPE+ particles

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    ENVLPE+
    

    Expression

    Mammalian

    Promoter

    2xCMV

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV_miniENVLPE

    Plasmid

    #232431

    Purpose

    Expression plasmid to produce miniENVLPE particles

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    miniENVLPE
    

    Expression

    Mammalian

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV5-Flag-XBP1s

    Plasmid

    #63680

    Purpose

    Mammalian expression of Flag tagged XBP1s

    Depositor

    Qingbo Xu, Lingfang Zeng

    Article

    Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

    Insert

    XBP1 (XBP1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    spliced

    Promoter

    CMV

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pD3sE_SC12

    Plasmid

    #219720

    Purpose

    DV3 E dimer with fusion loop mutation and increased expression

    Depositor

    Brian Kuhlman

    Article

    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi

    Insert

    DV3 sE SC12
    

    Tags

    8xHis and human serum albumin (HSA)

    Expression

    Mammalian

    Mutation

    G106D, A257W, T260R, F277W, A278P, G29K, T33V, A3…

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pD4sE_SC12

    Plasmid

    #219721

    Purpose

    DV4 stable E dimer with fusion loop mutation and increased expression

    Depositor

    Brian Kuhlman

    Article

    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi

    Insert

    DV4 sE SC12
    

    Tags

    8xHis and human serum albumin (HSA)

    Expression

    Mammalian

    Mutation

    G106D, A259W, S262R, F279W, A280P, G29K, T33V, A2…

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pD1sE_SC12

    Plasmid

    #219718

    Purpose

    DV1 E dimer with fusion loop mutation and increased expression

    Depositor

    Brian Kuhlman

    Article

    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi

    Insert

    DV1 sE SC12
    

    Tags

    8xHis and human serum albumin (HSA)

    Expression

    Mammalian

    Mutation

    G106D, A259W, T262R, F279W, A280P, S29K, T33V, A2…

    Available Since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pD2sE_SC12

    Plasmid

    #219719

    Purpose

    DV2 stable E dimer with fusion loop mutation and increased expression

    Depositor

    Brian Kuhlman

    Article

    Phan et al J Biol Chem. 2022 Jul;298(7):102079. doi

    Insert

    DV2 sE SC12
    

    Tags

    8xHis and human serum albumin (HSA)

    Expression

    Mammalian

    Mutation

    G106D, A259W, T262R, F279W, T280P, S29K, T33V, A2…

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQ262-ABE8e

    Plasmid

    #161523

    Purpose

    Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE8e mediated A-G base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

    Insert

    ABE8e-zSpCas9(D10A)
    

    Use

    CRISPR; Gateway compatible abe8e-zspcas9(d10a) en…

    Expression

    Plant

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV_mGL_PP7

    Plasmid

    #232440

    Purpose

    expression plasmid to produce mGreenLantern mRNA with a PP7 aptamer in the 3' UTR

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    mGreenLantern
    

    Expression

    Mammalian

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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