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pGGDestTol2LC-3sgRNA

Plasmid

#64241

Purpose

destination vector for three U6x:sgRNA cassettes

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available Since

May 5, 2015

Availability

Academic Institutions and Nonprofits only

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pGGDestTol2LC-1sgRNA

Plasmid

#64239

Purpose

destination vector for one U6x:sgRNA cassette

Depositor

Wenbiao Chen

Article

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Type

Empty backbone

Use

CRISPR

Available Since

May 5, 2015

Availability

Academic Institutions and Nonprofits only

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bRA66

Plasmid

#100952

Purpose

GAL1 driven Cas9. gRNA can be cloned into BplI sites.

Depositor

James Haber

Article

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-U6-empty

Plasmid

#140580

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Type

Empty backbone

Use

CRISPR

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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bRA89

Plasmid

#100950

Purpose

Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HPH marker

Depositor

James Haber

Article

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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GtCCR2_EYFP_pcDNA3.1

Plasmid

#159121

Purpose

This plasmid encodes GtCCR2 rhodopsin domain

Depositor

John Spudich

Article

Sineshchekov et al Proc Natl Acad Sci U S A. 2017 Nov 7;114

Insert

GtCCR2

Tags

EYFP

Expression

Mammalian

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-BRIC

Plasmid

#172338

Purpose

Bioluminescent Red Indicator for Calcium

Depositor

Huiwang Ai

Article

Tian et al Nat Commun. 2022 Jul 8;13(1):3967. doi:

Insert

BRIC

Expression

Mammalian

Promoter

CMV promoter

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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HA-Vim

Plasmid

#46315

Depositor

Xiangli Yang

Article

Lian et al J Biol Chem. 2009 Oct 30;284(44):30518-2

Insert

Vimentin (Vim Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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pGH810

Plasmid

#230849

Purpose

Ef1a-DivA-BE-T2A-BlastR-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DivA-BE

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3_Csx29

Plasmid

#197606

Purpose

Expresses Csx29 in mammalian cells

Depositor

Feng Zhang

Article

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

Csx29

Expression

Mammalian

Available Since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2L6

Plasmid

#15797

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2L6 (UBE2L6 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pTW357b

Plasmid

#227930

Purpose

Low-stringency accessory plasmid for selection

Depositor

Tina Wang

Article

Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

Inserts

gIII (recoded)
T7c

Expression

Bacterial

Mutation

R632S

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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bRA90

Plasmid

#100951

Purpose

Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. LEU2 marker

Depositor

James Haber

Article

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2H

Plasmid

#15752

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2H (UBE2H Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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bRA77

Plasmid

#100953

Purpose

GAL1 driven Cas9. gRNA can be cloned into BplI sites.

Depositor

James Haber

Article

Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST17-UbE2J2

Plasmid

#15794

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2J2 (UBE2J2 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
pAC1817_pCR8-gSMN2-EX

Plasmid

#118651

Purpose

Transient transfection; Expressed SMN2-EX CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

gSMN2-EX

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET24c(+)-EFTs

Plasmid

#31173

Depositor

Linda Spremulli

Article

Zhang et al FEBS Lett. 1996 Aug 12. 391(3):330-2.

Insert

E. coli elongation factor Ts

Tags

His

Expression

Bacterial

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-LambdaN-NbALFA

Plasmid

#207627

Purpose

Expresses LambdaN-NbALFA (ALFA-tag nanobody) fusion protein in mammalian cells (with a CMV promoter)

Depositor

Jay Shendure

Article

Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

Insert

LambdaN-NbALFA

Use

Synthetic Biology

Promoter

pCMV

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-CamkII-MacQ-mCitrine

Plasmid

#48762

Purpose

sensor

Depositor

Mark Schnitzer

Article

Gong et al Nat Commun. 2014 Apr 22;5:3674. doi: 10.

Insert

macQ-mCitrine

Use

Lentiviral

Expression

Mammalian

Mutation

mac-D139Q

Promoter

CamKIIa

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only

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