We narrowed to 9,861 results for: SUB;

1. pET28a-HsSENP3

   Plasmid

   #71467

   Purpose

   Encodes human full length SENP3. Suitable for in vitro transcription/translation (T7).

   Depositor

   Jorge Eduardo Azevedo

   Article

   Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

   Insert

   SUMO1/sentrin specific peptidase 3 (SENP3 Human)
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Gene optimised for E. coli expression (Genscript)

   Promoter

   T7

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. EKARdual

   Plasmid

   #99870

   Purpose

   Measure ERK activity by conformational change after ERK substrate phosphorylation and recognition of this phosphorylated substrate

   Depositor

   Marc Tramier

   Article

   Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

   Insert

   EKAR
   

   Use

   Tags

   ShadowG and mTFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Thy1 promoter construct

   Plasmid

   #20736

   Depositor

   Joshua Sanes

   Article

   Feng et al Neuron. 2000 Oct . 28(1):41-51.

   Insert

   Thy1 promoter (Thy1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. h-beta-ENaC

   Plasmid

   #83429

   Purpose

   full length human beta ENaC with V5 tag

   Depositor

   Christie Thomas

   Article

   Raikwar et al Am J Physiol Renal Physiol. 2008 May;294

   Insert

   human beta ENaC (SCNN1B Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET11a-hRanGAP1

   Plasmid

   #50941

   Purpose

   expression of full length untagged human RanGAP1 in E.coli

   Depositor

   Frauke Melchior

   Article

   Werner et al Mol Cell. 2012 May 11;46(3):287-98. doi:

   Insert

   RanGTPase activating protein 1 (RANGAP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3 MEKK1 WT

   Plasmid

   #12181

   Depositor

   Gary Johnson

   Article

   Widmann et al Mol Cell Biol. 1998 Apr . 18(4):2416-29.

   Insert

   MEKK1 (Map3k1 Mouse)
   

   Use

   Tags

   His and Xpress

   Expression

   Mammalian

   Mutation

   V1467L

   Promoter

   Available since

   Sept. 22, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. AKARdual

   Plasmid

   #99869

   Purpose

   Measure PKA activity by conformational change after PKA substrate phosphorylation and recognition of this phosphorylated substrate

   Depositor

   Marc Tramier

   Article

   Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

   Insert

   AKAR4
   

   Use

   Tags

   LSSmOrange and mKate2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETDuet RagA-RagC

   Plasmid

   #99641

   Purpose

   Bacterial expression of wildtype Rag GTPase dimer

   Depositor

   David Sabatini, Kuang Shen

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. lenti-ER-mTagBFP2

   Plasmid

   #216127

   Purpose

   Lentiviral vector for expressing mTagBFP2 localizing to the endoplasmic reticulum.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

   Insert

   EF1a-ProlactinEndoplasmicReticulumSignalSequence-mTagBFP2-KDEL-WPRE
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEJS613_pTetR-P2A-BFPnls/sgTelo

    Plasmid

    #108649

    Purpose

    Telomere-targeting Spy sgRNA under U6 promoter

    Depositor

    Erik Sontheimer

    Article

    Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

    Inserts

    Telomere-targeting sgRNA
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    No

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and hPGK

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-ATXN1-52Q

    Plasmid

    #32492

    Depositor

    Huda Zoghbi

    Article

    Cummings et al Nat Genet. 1998 Jun;19(2):148-54.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    insert contains a stretch of 52 Glutamines and no…

    Promoter

    CMV

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pHAGE DN Cullin5

    Plasmid

    #41916

    Depositor

    Stephen Elledge

    Article

    Emanuele et al Cell. 2011 Oct 14;147(2):459-74. doi: 10

    Insert

    CUL5 (CUL5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZac2.1 gfaABC1D-Ezr-eGFP

    Plasmid

    #176856

    Purpose

    Expresses Ezrin fused with eGFP at the C-terminus in astrocytes

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    Ezr-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    gfaABC1D

    Available since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGBW-m4046887

    Plasmid

    #145730

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pCX-SpiCee-NLS

    Plasmid

    #140900

    Purpose

    mammalian expression of SpiCee-NLS, a nucleus-restricted variant of the calcium buffer SpiCee that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

    Insert

    SpiCee-NLS
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19-Hph

    Plasmid

    #188741

    Purpose

    pUC19 plasmid (MCS intact) with Hygromycin selection marker

    Depositor

    Michael Bromley

    Article

    Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

    Insert

    Hygromycin
    

    Use

    Filamentous fungal expression

    Tags

    Expression

    Mutation

    Promoter

    gpdA

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry-FKBP-PI4KAC1001(D1957A)

    Plasmid

    #139312

    Purpose

    Recruitable PI4KA kinase-dead catalytic fragment

    Depositor

    Gerry Hammond

    Article

    Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    mCherry: FKBP1A(3-108): GGSA]4GG:PI4KA(1102-2103) (PI4KA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D1957A, G1986D

    Promoter

    Available since

    April 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFastBac1 Flag BRG1

    Plasmid

    #1957

    Depositor

    Robert Kingston

    Article

    Phelan et al Mol Cell 1999 Feb;3(2):247-53.

    Insert

    BRG1 (SMARCA4 Human)
    

    Use

    Tags

    flag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pHR-BTLA-mGFP

    Plasmid

    #180791

    Purpose

    Expression of human BTLA fused to mEGFP

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    BTLA (BTLA Human)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. px458_2A_GFP_sgRNA_RACK1

    Plasmid

    #127123

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    gRNA RACK1 (RACK1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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