We narrowed to 5,724 results for: chia

1. pPK1E-1

   Plasmid

   #48145

   Purpose

   Shuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1E

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. EC.SurA.pTYB1.a21-281&390-428.M231R

   Plasmid

   #15958

   Depositor

   David McKay

   Article

   Xu et al J Mol Biol. 2007 Oct 19. 373(2):367-81.

   Insert

   Escherichia coli surA delta PPIase domain 2
   

   Use

   Tags

   intein

   Expression

   Bacterial

   Mutation

   residues 282-389 deleted; M231R mutation

   Promoter

   Available since

   Nov. 9, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pGL4-UPRE-luc2P-Hygro

   Plasmid

   #101788

   Purpose

   Luciferase reporter plasmid containing three tandem repeats of the unfolded protein response element (UPRE)

   Depositor

   Seiichi Oyadomari

   Article

   Fujita et al Acta Med Okayama. 2020 Jun;74(3):199-208

   Insert

   3xUPRE
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   minimal TATA-box promoter with low basal activity

   Available since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL4-ERSE1-luc2P-Hygro

   Plasmid

   #101789

   Purpose

   Luferase reporter plasmid containing three tandem repeats of the ER stress response element-I (ERSE-I)

   Depositor

   Seiichi Oyadomari

   Article

   Fujita et al Acta Med Okayama. 2020 Jun;74(3):199-208

   Insert

   3xERSE-I
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   minimal TATA-box promoter with low basal activity

   Available since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL4-ERSE2-luc2P-Hygro

   Plasmid

   #101790

   Purpose

   Luciferase reporter plasmid containing three tandem repeats of the ER stress response element-II (ERSE-II)

   Depositor

   Seiichi Oyadomari

   Article

   Fujita et al Acta Med Okayama. 2020 Jun;74(3):199-208

   Insert

   3xERSE-II
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   minimal TATA-box promoter with low basal activity

   Available since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTP-PCR C3

   Plasmid

   #189620

   Purpose

   Cloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteria

   Depositor

   Bogumil Karas

   Article

   Cochrane et al Biology (Basel). 2020 Oct 26;9(11). pii:

   Insert

   Reduced Mitochondrial Genome of Thalassiosira pseudonana
   

   Use

   Synthetic Biology; Diatom expression

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Removed repeat region, 10-kbp deletion, and minor…

   Promoter

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHL 1158

   Plasmid

   #53007

   Purpose

   pLtetO-1:RhyB, pCon:DsrA, pCon:ompC::mCherry, pLlacO-1:sodB::gfp

   Depositor

   Han Lim

   Article

   Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

   Inserts

   RhyB sRNA
   DsrA sRNA
   ompC
   sodB
   

   Use

   Synthetic Biology

   Tags

   gfp and mCherry

   Expression

   Bacterial

   Mutation

   contains -56 to +141 relative to start codon and …

   Promoter

   pCon, pLlacO-1, and pLtetO-1

   Available since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pMJ922

   Plasmid

   #78312

   Purpose

   Expression of His6-MBP-tagged Cas9-NLS-EGFP protein in E. coli

   Depositor

   Martin Jinek

   Article

   Burger et al Development. 2016 Apr 29. pii: dev.13480

   Insert

   SpCas9
   

   Use

   Tags

   HA-2xNLS-EGFP-NLS and His6-MBP-TEV

   Expression

   Bacterial

   Mutation

   human codon-optimized gene sequence

   Promoter

   T7

   Available since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC015 - dLwCas13a-NF

   Plasmid

   #91905

   Purpose

   Expresses negative-feedback dLwCas13a for mammalian RNA targeting/imaging

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   dLwCas13a
   

   Use

   CRISPR

   Tags

   NLS and eGFP, ZF CCR5TC, KRAB-A, NLS

   Expression

   Mammalian

   Mutation

   R474A and R1046A

   Promoter

   CAG

   Available since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCOLA-Gent-EM7-Erv1p-DsbC

    Plasmid

    #202485

    Purpose

    Expresses sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) constitutively

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    sulfhydryl oxidase (Erv1p) and disulfide bond isomerase (dsbC) (ERV1 Budding Yeast, Escherichia coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    EM7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pC034 - LwCas13a-msfGFP-2A-Blast

    Plasmid

    #91924

    Purpose

    Expresses active LwCas13a for mammalian RNA targeting with Blasticidin selection marker

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LwCas13a
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS and msfGFP-NLS-3xHA, P2A, Blasticidin

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21a-TEX264_wt-His6

    Plasmid

    #220214

    Purpose

    Bacterial expression of wild type TEX264, C-terminal His6 tag

    Depositor

    Kristijan Ramadan

    Article

    Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

    Insert

    TEX264 (TEX264 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-ATG8(416)/GFP-AUT7(416)

    Plasmid

    #49425

    Purpose

    Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS416 vector.

    Depositor

    Daniel Klionsky

    Article

    Guan et al Mol Biol Cell. 2001 Dec;12(12):3821-38.

    Insert

    autophagy-related 8 (ATG8 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    ATG8

    Available since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. lentiCRISPR-v2-HygR-EGFP

    Plasmid

    #167188

    Purpose

    Lentiviral expression of sgRNA with hygromycin resistance gene and EGFP

    Depositor

    Yin Shen

    Article

    Ren et al Sci Adv. 2021 Sep 17;7(38):eabi4360. doi

    Insert

    Hygromycin B phosphotransferase and EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pORTMAGE-1

    Plasmid

    #72680

    Purpose

    Same as pORTMAGE-2, but with a non-optimized RBS before mutant MutL, thus lower level of expression of the enzyme.

    Depositor

    Csaba Pál

    Article

    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p

    Insert

    mutL E32K
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    lower strength RBS before mutL E32K allele than i…

    Promoter

    Available since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pCMV4a-SIRT2-Flag

    Plasmid

    #102623

    Purpose

    Expresses full length SIRT2 isoform 1 in mammalian cells

    Depositor

    Hening Lin

    Article

    Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

    Insert

    Sirtuin 2 (SIRT2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJM230 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-lacZ)

    Plasmid

    #110560

    Purpose

    miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-lacZ insert

    Depositor

    Joanna Goldberg

    Article

    Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

    Insert

    stRBS-lacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rhaSR-PrhaBAD

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC001 - huLshC2C2-MBP for bacterial expression

    Plasmid

    #79150

    Purpose

    Expresses human codon-optimized LshC2c2 for purification in E. coli

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

    Insert

    C2c2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. HA-FOXO3a TM-ER

    Plasmid

    #8353

    Depositor

    Michael Greenberg

    Article

    Tran et al Science 2002 Apr 19;296(5567):530-4.

    Insert

    FOXO3a (FOXO3 Human)
    

    Use

    Tags

    HA (YDVPDYASL)

    Expression

    Mammalian

    Mutation

    T32A, S253A, S315A, mouse estrogen receptor (G525…

    Promoter

    Available since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

20. pAX1_HS_gpN:SnoopTag

    Plasmid

    #225195

    Purpose

    Used for adding C-terminal SnoopTag to the E. coli HS P2 phage major capsid protein

    Depositor

    Travis Wiles

    Article

    Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

    Insert

    gpN homology arms and SnoopTag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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