We narrowed to 5,023 results for: puc

1. pAD03 S. bayanus telomerase RNA KBS

   Plasmid

   #61892

   Purpose

   Plasmid for run-off in vitro transcription of Saccharomyces bayanus KBS RNA

   Depositor

   Thomas Cech

   Article

   Dalby et al RNA. 2013 Jun;19(6):841-51. doi: 10.1261

   Insert

   Telomerase RNA hairpin
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pAD05 S. castellii telomerase RNA KBS

   Plasmid

   #61894

   Purpose

   Plasmid for run-off in vitro transcription of Saccharomyces castellii KBS RNA

   Depositor

   Thomas Cech

   Article

   Dalby et al RNA. 2013 Jun;19(6):841-51. doi: 10.1261

   Insert

   Telomerase RNA hairpin
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pAD02 S. paradoxus telomerase RNA KBS

   Plasmid

   #61891

   Purpose

   Plasmid for run-off in vitro transcription of Saccharomyces paradoxus KBS RNA

   Depositor

   Thomas Cech

   Article

   Dalby et al RNA. 2013 Jun;19(6):841-51. doi: 10.1261

   Insert

   Telomerase RNA hairpin
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pAD04 S. arboricola telomerase RNA KBS

   Plasmid

   #61893

   Purpose

   Plasmid for run-off in vitro transcription of Saccharomyces arboricola KBS RNA

   Depositor

   Thomas Cech

   Article

   Dalby et al RNA. 2013 Jun;19(6):841-51. doi: 10.1261

   Insert

   Telomerase RNA hairpin
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

5. LI D-E 1-10GFP

   Plasmid

   #54452

   Depositor

   Martin Parniske

   Article

   Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

   Insert

   16 aa GS linker + superfolder GFP 1-10 domain
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. LI B-C 6xHIS

   Plasmid

   #54446

   Depositor

   Martin Parniske

   Article

   Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

   Insert

   polyhistidine (6x) tag
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LI C-D YFP c1

   Plasmid

   #54415

   Depositor

   Martin Parniske

   Article

   Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

   Insert

   YFP Venus
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LI D-E C-NES c1

   Plasmid

   #54429

   Depositor

   Martin Parniske

   Article

   Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

   Insert

   NES of heat stable inhibitor (PKI)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGGB005

   Plasmid

   #48822

   Purpose

   Provides the SV40 nuclear localization signal as GreenGate N-terminal tag module.

   Depositor

   Jan Lohmann

   Article

   Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

   Insert

   SV40 nuclear localization signal
   

   Use

   Golden gate compatible cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGGA003

    Plasmid

    #48814

    Purpose

    Provides the WUS promoter as GreenGate module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    WUS promoter
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEFS-ccdB-12X MS2_mPGK-Puro

    Plasmid

    #177809

    Purpose

    Assembly backbone for tagging RNA transcript with a 12X tandem MS2 stemloop at the 3'

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Sci Rep. 2022 Jan 27;12(1):1513. doi: 10

    Type

    Empty backbone

    Use

    Tags

    12X MS2 stem loops

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. qTAG-C-dTAG-Puro

    Plasmid

    #207727

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pC0042-PguCas13b crRNA backbone

    Plasmid

    #103853

    Purpose

    For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    U6-PguCas13b DR-BbsI-BbsI-polyT
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEF1α-Cre-2A-Puro

    Plasmid

    #207743

    Purpose

    Expression of the Cre recombinase linked to a puro selectable marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-2A-Puro
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. qTAG-C-mStayGold-Puro

    Plasmid

    #227253

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEF1α-Cre-2A-moxGFP

    Plasmid

    #207744

    Purpose

    Expression of the Cre recombinase linked to a moxGFP fluorescent marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-PGK-moxGFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. qTAG-C-mScarlet-Puro

    Plasmid

    #207722

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pC0041-RanCas13b crRNA backbone

    Plasmid

    #103852

    Purpose

    For cloning of guide RNAs compatible with RanCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac.

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    U6-RanCas13b DR-BbsI-BbsI-polyT
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. qTAG-C-3xFLAG-Puro

    Plasmid

    #207728

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. qTAG-C-miniTurbo-Puro

    Plasmid

    #207725

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miniTurbo-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge