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Showing: 2921 - 2940 of 3371 results

  5. SPDK3888(TRV-RNA2-pPEBV-MCS-AtIleu-tRNA)

    Plasmid
    #149276
    Purpose
    Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs
    Depositor
    Savithramma Dinesh-Kumar
    Article
    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41
    Insert
    Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence
    Use
    T-dna vector
    Tags
    Expression
    Mutation
    T2318C (DNA) in PEBV coat protein sequence
    Promoter
    Available since
    June 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    149276-plasmid-map-sequence-id-300510

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  6. pEntr_Myc-BirA-G3-ER

    Plasmid
    #173814
    Purpose
    Promiscuous biotin ligase (BirA-G3) targeted to ER lumen in Gateway entry vector. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage site
    Depositor
    Norbert Perrimon
    Article
    Droujinine et al Nat Commun. 2021 Apr 22;12(1):2382. doi:
    Insert
    Myc-BirA-G3-ER
    Use
    Gateway shuttle vector
    Tags
    Myc
    Expression
    Mutation
    Promoter
    Available since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    173814-plasmid-map-sequence-id-340315

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  7. pCK306

    Plasmid
    #110544
    Purpose
    rhaBAD, a rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria.
    Depositor
    John Heap
    Article
    Kelly et al ACS Synth Biol. 2018 Apr 2. doi: 10.1021
    Inserts
    PrhaBAD
    yfp
    rhaS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    N/A, PrhaBAD, and kanR promoter (upstream of kanR…
    Available since
    June 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    110544-plasmid-map-sequence-id-212164

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  8. pIA33

    Plasmid
    #120803
    Purpose
    E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp
    Depositor
    Craig Ellermeier, David S. Weiss
    Article
    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-
    Inserts
    deactivated nuclease dCas9, codon-optimized for C. difficile
    single guide RNA targeting red fluorescent protein
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Base-pairing region targets red fluorescent prote…
    Promoter
    Pgdh and Pxyl
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    120803-plasmid-map-sequence-id-235316

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  16. attB53-Pac-TRE-3xFlag-Neurog1-attB53

    Plasmid
    #183613
    Purpose
    RMCE vector to shuttle puromycin resistance and TRE-3xFlag-Neurog1 cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).
    Depositor
    Sally Lowell
    Article
    Malaguti et al Development. 2022 May 26. pii: 275525. d
    Insert
    Neurog1 (Neurog1 Mouse)
    Use
    Rmce
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183613-plasmid-map-sequence-id-361602

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  17. pFrt-invCAG-ires-Luc

    Plasmid
    #63576
    Purpose
    Shuttle vector compatible with Flp-mediated (Flp-in) transgene integration that allows for conditional cDNA and Luc expression upon integration. The vector contains an FRT site and two mut loxP sites.
    Depositor
    Ivo Huijbers
    Article
    Huijbers et al Nat Protoc. 2015 Nov;10(11):1755-85. doi
    Insert
    Stop-Lox71-IRES-Luciferase-pA-FRT-Lox66-reverseCAG
    Use
    Cre/Lox; Frt/flpe recombination mediated insertion
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    April 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    63576-plasmid-map-sequence-id-105724

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  18. pTU1-A-pdh_RiboJ_mCherry_Bba_B0015

    Plasmid
    #107581
    Purpose
    B. megaterium DSM319 pdh promoter, mCherry (Bacillus codon optimised) for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)
    Depositor
    Paul Freemont
    Article
    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p
    Insert
    mCherry
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pdh promoter Bacillus megaterium DSM319
    Available since
    April 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    107581-plasmid-map-sequence-id-209155

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  20. SPDK3889(TRV-RNA2-pPEBV-MCS-AtMet-tRNA)

    Plasmid
    #149277
    Purpose
    Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs
    Depositor
    Savithramma Dinesh-Kumar
    Article
    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41
    Insert
    Modified TRV RNA2 with PEBV promoter and AtMet-tRNA mobile RNA sequence
    Use
    T-dna vector
    Tags
    Expression
    Mutation
    T2318C (DNA) in PEBV coat protein sequence
    Promoter
    Available since
    June 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    149277-plasmid-map-sequence-id-300516

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Showing: 2921 - 2940 of 3371 results