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We narrowed to 5,523 results for: golden gate

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Showing: 2941 - 2960 of 5523 results

  1. DVA_pBR322_AG

    Plasmid
    #217607
    Purpose
    Destination vector with ampicillin resistance and pBR322 origin of replication. Carries A-G CIDAR MoClo overhangs for level 0 or 2 construct cloning.
    Depositor
    Wei Huang
    Publication
    Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    None
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217607-plasmid-map-sequence-id-442574

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  2. DVC_pBR322_AF

    Plasmid
    #217598
    Purpose
    Destination vector with chloramphenicol resistance and pBR322 origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.
    Depositor
    Wei Huang
    Publication
    Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    None
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217598-plasmid-map-sequence-id-442573

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  3. DVC_pBR322_FG

    Plasmid
    #217600
    Purpose
    Destination vector with chloramphenicol resistance and pBR322 origin of replication. Carries F-G CIDAR MoClo overhangs for level 1 construct cloning.
    Depositor
    Wei Huang
    Publication
    Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    None
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217600-plasmid-map-sequence-id-442571

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  4. DVC_pBR322_AE

    Plasmid
    #217597
    Purpose
    Destination vector with chloramphenicol resistance and pBR322 origin of replication. Carries A-E CIDAR MoClo overhangs for level 1 construct cloning.
    Depositor
    Wei Huang
    Publication
    Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    None
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217597-plasmid-map-sequence-id-442569

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  5. DVC_p15A_FG

    Plasmid
    #217590
    Purpose
    Destination vector with chloramphenicol resistance and p15A origin of replication. Carries F-G CIDAR MoClo overhangs for level 1 construct cloning.
    Depositor
    Wei Huang
    Publication
    Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    None
    Available since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217590-plasmid-map-sequence-id-442567

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  7. L1T18 - T2PsB/p35S preswitched (mTagBFP2, mCherry, NeonGreen)

    Plasmid
    #219690
    Purpose
    Level 1 preswitched History-dependent target, switch with Bxb1, p35S promoter, and output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
    Depositor
    Jennifer Nemhauser
    Insert
    T2PsB/p35S (mTagBFP2, mCherry, NeonGreen)
    Expression
    Plant
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219690-plasmid-map-sequence-id-435857

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  8. L1T9 - T2PB/p35S (mTagBFP2, mCherry, NeonGreen)

    Plasmid
    #219686
    Purpose
    Level 1 History-dependent target, switch PhiC31 then Bxb1, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
    Depositor
    Jennifer Nemhauser
    Insert
    T2PB/p35S (mTagBFP2, mCherry, NeonGreen)
    Expression
    Plant
    Available since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219686-plasmid-map-sequence-id-436048

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  9. pNK116

    Plasmid
    #219742
    Purpose
    MoClo-compatible Level 0 promoterless vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 codon-optimised for expression in Nicotiana benthamiana
    Depositor
    Alexander Mishin
    Publication
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Insert
    mutant of fungal hispidin-3-hydroxylase
    Use
    Synthetic Biology
    Mutation
    D37E, V181I, S323M, M385K
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219742-plasmid-map-sequence-id-437105

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  10. pNK5664

    Plasmid
    #219747
    Purpose
    DVK_AF CIDAR vector encoding mutant of Neonothopanus nambi luciferase nnLuz_v4 under control of CMV promoter, for mammalian expression
    Depositor
    Alexander Mishin
    Publication
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pCMV - nnLuz_v4 - tSV40
    Use
    Luciferase
    Expression
    Mammalian
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219747-plasmid-map-sequence-id-437128

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  11. pNK3203

    Plasmid
    #219749
    Purpose
    DVK_AF CIDAR vector encoding hispidin-synthase from Mycena citricolor under control of CMV promoter, for mammalian expression
    Depositor
    Alexander Mishin
    Publication
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pCMV - mcitHispS - tSV40
    Expression
    Mammalian
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219749-plasmid-map-sequence-id-437134

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  12. pBH502

    Plasmid
    #215781
    Purpose
    NBU2 backbone with LacI(YQR) and P(O1)_sgRNA targeting Nanoluc
    Depositor
    Corey Wilson
    Publication
    Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:
    Inserts
    LacI(YQR)
    sgRNA-ribozyme cassette
    Nanoluc
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    Bt1311, P(O1), and cfxA
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    215781-plasmid-map-sequence-id-430541

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  13. pBH504

    Plasmid
    #215782
    Purpose
    NBU1 backbone with constitutive dCas9, LacI(YQR), P(O1)_sgRNA targeting B. theta amylopectin SusC gene
    Depositor
    Corey Wilson
    Publication
    Huang et al Nat Commun. 2022 Jul 6;13(1):3901. doi:
    Inserts
    LacI(YQR)
    sgRNA-ribozyme cassette
    dCas9
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    Bt1311, P1, and P(O1)
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    215782-plasmid-map-sequence-id-430549

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  16. WT_S1-H1

    Plasmid
    #218392
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, and hnRNPA1 motif-rich region in second intron
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available since
    June 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    218392-plasmid-map-sequence-id-436407

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  18. pSCL758

    Plasmid
    #219504
    Purpose
    Express Eco3RT from a CAG promoter; also express an msr and HEK3, FANCF and EMX1 retron msd donor-gRNAs from a single H1 promoter, separated by tRNA-Cys-GCA sequences
    Depositor
    Seth Shipman
    Publication
    Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    msr and HEK3, FANCF and EMX1 retron msd donor-gRNAs from a single H1 promoter, separated by tRNA-Cys-GCA sequences
    Expression
    Mammalian
    Promoter
    H1
    Available since
    May 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    219504-plasmid-map-sequence-id-487657

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  20. MUT_S1-H1

    Plasmid
    #218394
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218394-plasmid-map-sequence-id-433909

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Showing: 2941 - 2960 of 5523 results